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- W2801157296 abstract "Capacitance measurements provide unique insights into the thickness, compressibility, and composition of large-area membrane bilayers and are used here in addition to demonstrate the successful incorporation of model ion channels. The simultaneous ability to control the bilayer size, manipulate tension, and optically monitor and electrically stimulate freestanding membranes enables precise determination of their specific capacitance and thickness across a wide range of areas. We confirm that membranes formed by this recently developed technique have capacitive properties similar to those formed by existing protocols, including solvent-free approaches, and discuss the effect using either hexadecane or squalene as the oil solvent. The results obtained here are relevant for other methods where lipid membranes are reconstituted from a bulk oil solvent. Because biological membranes have a diverse phospholipid profile, we show that the technique can successfully reconstitute membranes with binary composition mixtures. As an outlook, we show the capability of model membrane proteins, specifically α-hemolysin and alamethicin, to be incorporated into the formed bilayers and measure ion transport." @default.
- W2801157296 created "2018-05-17" @default.
- W2801157296 creator A5035331501 @default.
- W2801157296 creator A5056940411 @default.
- W2801157296 creator A5071592118 @default.
- W2801157296 date "2018-05-01" @default.
- W2801157296 modified "2023-10-16" @default.
- W2801157296 title "Toward Realistic Large-Area Cell Membrane Mimics: Excluding Oil, Controlling Composition, and Including Ion Channels" @default.
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- W2801157296 doi "https://doi.org/10.1021/acs.langmuir.8b00837" @default.
- W2801157296 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29715042" @default.
- W2801157296 hasPublicationYear "2018" @default.
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