Matches in SemOpenAlex for { <https://semopenalex.org/work/W2801174013> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2801174013 endingPage "323" @default.
- W2801174013 startingPage "323" @default.
- W2801174013 abstract "Nanotechnology is one of the fastest growing technologies in this era. Gold nanoparticle (AuNP) based immunoassays have been performed on the basis of antigen-antibody interaction using AuNP antibody conjugates. Lateral flow immunoassays(LFA) which are also based on AuNP antibody conjugates are useful innovation in nanotechnology and widely applied in medicine and research fields. However, there are some limitations of the present LFA kits such as sensitivity and stability. In the study, we showed the result of covalent conjugation of anti-rotavirus antibody and AuNP for generating a lateral flow immunoassay strip to detect rotavirus in fecal samples. The suitable conditions for coating polyethylene glycol (PEG) on the surface of AuNP were 10.0 mM PEG for 3 hours at room temperature (25 oC). Optimized conditions for covalent conjugation of antibody and AuNP were pH 4.0, 0.1 mg antibody/conjugate, 0.01 mM reactant EDC/NHS [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/(N-hydroxy sulfosuccinimide]. The coupling reaction was carried out at room temperature for 90 min. The conjugate pad, antibody immobilized nitrocellulose membrane strip were created with investigated conditions for generating an LFA test strip. The limit of detection of LFA test strip was determined by 1.6 × 105 virus particles/ml, three times lower than that of Rotaclone kit (UK). The generated strip could be used to detect rotavirus in fecal sample of patient." @default.
- W2801174013 created "2018-05-17" @default.
- W2801174013 creator A5045562811 @default.
- W2801174013 date "2018-03-21" @default.
- W2801174013 modified "2023-09-25" @default.
- W2801174013 title "COVALENT CONJUGATION OF ANTIBODY AND GOLD NANOPARTICLE FOR DEVELOPMENT OF LATERAL FLOW IMMUNOASSAY TEST STRIP" @default.
- W2801174013 doi "https://doi.org/10.15625/2525-2518/54/4a/12009" @default.
- W2801174013 hasPublicationYear "2018" @default.
- W2801174013 type Work @default.
- W2801174013 sameAs 2801174013 @default.
- W2801174013 citedByCount "0" @default.
- W2801174013 crossrefType "journal-article" @default.
- W2801174013 hasAuthorship W2801174013A5045562811 @default.
- W2801174013 hasBestOaLocation W28011740131 @default.
- W2801174013 hasConcept C10138342 @default.
- W2801174013 hasConcept C104819515 @default.
- W2801174013 hasConcept C119128265 @default.
- W2801174013 hasConcept C134306372 @default.
- W2801174013 hasConcept C13965031 @default.
- W2801174013 hasConcept C155672457 @default.
- W2801174013 hasConcept C159654299 @default.
- W2801174013 hasConcept C162324750 @default.
- W2801174013 hasConcept C171250308 @default.
- W2801174013 hasConcept C178790620 @default.
- W2801174013 hasConcept C180577832 @default.
- W2801174013 hasConcept C185592680 @default.
- W2801174013 hasConcept C188027245 @default.
- W2801174013 hasConcept C192562407 @default.
- W2801174013 hasConcept C197336794 @default.
- W2801174013 hasConcept C203014093 @default.
- W2801174013 hasConcept C2775997276 @default.
- W2801174013 hasConcept C2776964284 @default.
- W2801174013 hasConcept C2778954975 @default.
- W2801174013 hasConcept C2780420688 @default.
- W2801174013 hasConcept C33923547 @default.
- W2801174013 hasConcept C41625074 @default.
- W2801174013 hasConcept C43617362 @default.
- W2801174013 hasConcept C54400483 @default.
- W2801174013 hasConcept C55493867 @default.
- W2801174013 hasConcept C86803240 @default.
- W2801174013 hasConceptScore W2801174013C10138342 @default.
- W2801174013 hasConceptScore W2801174013C104819515 @default.
- W2801174013 hasConceptScore W2801174013C119128265 @default.
- W2801174013 hasConceptScore W2801174013C134306372 @default.
- W2801174013 hasConceptScore W2801174013C13965031 @default.
- W2801174013 hasConceptScore W2801174013C155672457 @default.
- W2801174013 hasConceptScore W2801174013C159654299 @default.
- W2801174013 hasConceptScore W2801174013C162324750 @default.
- W2801174013 hasConceptScore W2801174013C171250308 @default.
- W2801174013 hasConceptScore W2801174013C178790620 @default.
- W2801174013 hasConceptScore W2801174013C180577832 @default.
- W2801174013 hasConceptScore W2801174013C185592680 @default.
- W2801174013 hasConceptScore W2801174013C188027245 @default.
- W2801174013 hasConceptScore W2801174013C192562407 @default.
- W2801174013 hasConceptScore W2801174013C197336794 @default.
- W2801174013 hasConceptScore W2801174013C203014093 @default.
- W2801174013 hasConceptScore W2801174013C2775997276 @default.
- W2801174013 hasConceptScore W2801174013C2776964284 @default.
- W2801174013 hasConceptScore W2801174013C2778954975 @default.
- W2801174013 hasConceptScore W2801174013C2780420688 @default.
- W2801174013 hasConceptScore W2801174013C33923547 @default.
- W2801174013 hasConceptScore W2801174013C41625074 @default.
- W2801174013 hasConceptScore W2801174013C43617362 @default.
- W2801174013 hasConceptScore W2801174013C54400483 @default.
- W2801174013 hasConceptScore W2801174013C55493867 @default.
- W2801174013 hasConceptScore W2801174013C86803240 @default.
- W2801174013 hasIssue "4A" @default.
- W2801174013 hasLocation W28011740131 @default.
- W2801174013 hasOpenAccess W2801174013 @default.
- W2801174013 hasPrimaryLocation W28011740131 @default.
- W2801174013 hasRelatedWork W1979843943 @default.
- W2801174013 hasRelatedWork W2026268156 @default.
- W2801174013 hasRelatedWork W2044855482 @default.
- W2801174013 hasRelatedWork W2101403832 @default.
- W2801174013 hasRelatedWork W2346848998 @default.
- W2801174013 hasRelatedWork W2361358056 @default.
- W2801174013 hasRelatedWork W2466255286 @default.
- W2801174013 hasRelatedWork W2601924554 @default.
- W2801174013 hasRelatedWork W2794254150 @default.
- W2801174013 hasRelatedWork W2801174013 @default.
- W2801174013 hasVolume "54" @default.
- W2801174013 isParatext "false" @default.
- W2801174013 isRetracted "false" @default.
- W2801174013 magId "2801174013" @default.
- W2801174013 workType "article" @default.