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• W2801264231 abstract "In this study we designed an ultrasensitive electrochemical immunosensor for RACK 1 detection using 11-cyanoundecyltrimethoxysilane (11-CUTMS) as a immobilization matrix to immobilize biorecognition element. The used silane agent (11-CUTMS) provides a favorable platform for efficient loading of anti-RACK 1 antibody. The effective loading of the biorecognition element on the 11-CUTMS matrix was monitored by scanning electron microscopy (SEM), atomic force microscopy (AFM) images and fourier transform infrared spectroscopy (FTIR) spectra. The electrochemical characterization of the immunosensor was performed by using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Moreover, biorecognition interaction between anti-RACK1 antibodies and RACK1 antigens was monitored by using single frequency technique (SFI). The operating conditions, calibration curves obtained during optimization of experiments and reproducibility of the proposed impedimetric RACK1 biosensor are also investigated and discussed. The electrochemical immunosensor illustrated a sensitive response to RACK 1 antigen with detection limit of 10.8 fg/mL and in the linear range of 0.036–2.278 pg/mL (R2 = 0.999). Owing to high specificity, good reproducibility, long stability and reusability, the fabricated immunosensor will provide a sensitive, selective approach to RACK 1 detection. Furthermore, the practical applicability in human serum samples were investigated with a satisfactory result." @default.
• W2801264231 created "2018-05-17" @default.
• W2801264231 creator A5007134941 @default.
• W2801264231 creator A5012445077 @default.
• W2801264231 creator A5015302400 @default.
• W2801264231 date "2018-09-01" @default.
• W2801264231 modified "2023-10-03" @default.
• W2801264231 title "A label-free electrochemical biosensor for direct detection of RACK 1 by using disposable, low-cost and reproducible ITO based electrode" @default.
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• W2801264231 doi "https://doi.org/10.1016/j.aca.2018.04.031" @default.
• W2801264231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29776548" @default.
• W2801264231 hasPublicationYear "2018" @default.
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