Matches in SemOpenAlex for { <https://semopenalex.org/work/W2801301596> ?p ?o ?g. }
- W2801301596 endingPage "996" @default.
- W2801301596 startingPage "986" @default.
- W2801301596 abstract "Incomplete or aberrant reprogramming of nuclear genome is one of the major problems in somatic cell nuclear transfer. In this study, we studied the effect of histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (CBHA) on in vitro development of buffalo embryos produced by Hand-made cloning. Cloned embryos were treated with CBHA (0, 5, 10, 20 or 50 μM) for 10 hr from the start of reconstruction till activation. At 10 μM, but not at other concentrations examined, CBHA increased (p < .05) the blastocyst rate (63.77 ± 3.97% vs 48.63 ± 3.55%) and reduced (p < .05) the apoptotic index of the cloned blastocysts (8.91 ± 1.94 vs 4.36 ± 1.08) compared to untreated controls, to levels similar to those in IVF blastocysts (4.78 ± 0.74). CBHA treatment, at all the concentrations examined, increased (p < .05) the global level of H3K9ac in cloned blastocysts than in untreated controls to that observed in IVF blastocysts. Treatment with CBHA (10 μM) decreased (p < .05) the global level of H3K27me3 in cloned blastocysts than in untreated controls but it was still higher (p < .05) than in IVF blastocysts. CBHA (10 μM) treatment increased (p < .05) the relative expression level of pluripotency-related genes OCT-4 and NANOG, and anti-apoptotic gene BCL-XL, and decreased (p < .05) that of pro-apoptotic gene BAX than in untreated controls but did not affect the relative expression level of apoptosis-related genes p53 and CASPASE3 and epigenetics-related genes DNMT1, DNMT3a and HDAC1. These results suggest that treatment of cloned embryos with 10 μM CBHA improves the blastocyst rate, reduces the level of apoptosis and alters the epigenetic status and gene expression pattern." @default.
- W2801301596 created "2018-05-17" @default.
- W2801301596 creator A5014190214 @default.
- W2801301596 creator A5026733261 @default.
- W2801301596 creator A5026946514 @default.
- W2801301596 creator A5028800318 @default.
- W2801301596 creator A5042952124 @default.
- W2801301596 creator A5050751080 @default.
- W2801301596 creator A5063454480 @default.
- W2801301596 creator A5074647273 @default.
- W2801301596 date "2018-05-07" @default.
- W2801301596 modified "2023-10-18" @default.
- W2801301596 title "m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (<i>Bubalus bubalis</i> ) embryos" @default.
- W2801301596 cites W1515703466 @default.
- W2801301596 cites W1638097072 @default.
- W2801301596 cites W1966990812 @default.
- W2801301596 cites W1968987470 @default.
- W2801301596 cites W1969367571 @default.
- W2801301596 cites W1970289162 @default.
- W2801301596 cites W1988927816 @default.
- W2801301596 cites W1999015942 @default.
- W2801301596 cites W2000837763 @default.
- W2801301596 cites W2001070832 @default.
- W2801301596 cites W2002435383 @default.
- W2801301596 cites W2007497552 @default.
- W2801301596 cites W2010765881 @default.
- W2801301596 cites W2016946551 @default.
- W2801301596 cites W2019803568 @default.
- W2801301596 cites W2035529579 @default.
- W2801301596 cites W2038531539 @default.
- W2801301596 cites W2038967811 @default.
- W2801301596 cites W2047015004 @default.
- W2801301596 cites W2048886301 @default.
- W2801301596 cites W2059950394 @default.
- W2801301596 cites W2074781740 @default.
- W2801301596 cites W2089182240 @default.
- W2801301596 cites W2093155802 @default.
- W2801301596 cites W2105718307 @default.
- W2801301596 cites W2105916870 @default.
- W2801301596 cites W2106644837 @default.
- W2801301596 cites W2107277218 @default.
- W2801301596 cites W2109207015 @default.
- W2801301596 cites W2110015307 @default.
- W2801301596 cites W2115811412 @default.
- W2801301596 cites W2138589405 @default.
- W2801301596 cites W2147370015 @default.
- W2801301596 cites W2149760791 @default.
- W2801301596 cites W2160098318 @default.
- W2801301596 cites W2160542053 @default.
- W2801301596 cites W2163271530 @default.
- W2801301596 cites W2163798466 @default.
- W2801301596 cites W2519697816 @default.
- W2801301596 cites W2551506754 @default.
- W2801301596 cites W2603127474 @default.
- W2801301596 cites W996260659 @default.
- W2801301596 doi "https://doi.org/10.1111/rda.13198" @default.
- W2801301596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29733525" @default.
- W2801301596 hasPublicationYear "2018" @default.
- W2801301596 type Work @default.
- W2801301596 sameAs 2801301596 @default.
- W2801301596 citedByCount "13" @default.
- W2801301596 countsByYear W28013015962018 @default.
- W2801301596 countsByYear W28013015962019 @default.
- W2801301596 countsByYear W28013015962020 @default.
- W2801301596 countsByYear W28013015962021 @default.
- W2801301596 countsByYear W28013015962022 @default.
- W2801301596 countsByYear W28013015962023 @default.
- W2801301596 crossrefType "journal-article" @default.
- W2801301596 hasAuthorship W2801301596A5014190214 @default.
- W2801301596 hasAuthorship W2801301596A5026733261 @default.
- W2801301596 hasAuthorship W2801301596A5026946514 @default.
- W2801301596 hasAuthorship W2801301596A5028800318 @default.
- W2801301596 hasAuthorship W2801301596A5042952124 @default.
- W2801301596 hasAuthorship W2801301596A5050751080 @default.
- W2801301596 hasAuthorship W2801301596A5063454480 @default.
- W2801301596 hasAuthorship W2801301596A5074647273 @default.
- W2801301596 hasConcept C104317684 @default.
- W2801301596 hasConcept C107459253 @default.
- W2801301596 hasConcept C145103041 @default.
- W2801301596 hasConcept C150194340 @default.
- W2801301596 hasConcept C153911025 @default.
- W2801301596 hasConcept C159399396 @default.
- W2801301596 hasConcept C16685009 @default.
- W2801301596 hasConcept C18903297 @default.
- W2801301596 hasConcept C190283241 @default.
- W2801301596 hasConcept C196843134 @default.
- W2801301596 hasConcept C207701140 @default.
- W2801301596 hasConcept C2776202225 @default.
- W2801301596 hasConcept C2778177303 @default.
- W2801301596 hasConcept C2778305200 @default.
- W2801301596 hasConcept C2779867394 @default.
- W2801301596 hasConcept C41091548 @default.
- W2801301596 hasConcept C54355233 @default.
- W2801301596 hasConcept C64927066 @default.
- W2801301596 hasConcept C71924100 @default.
- W2801301596 hasConcept C77255625 @default.
- W2801301596 hasConcept C86803240 @default.
- W2801301596 hasConcept C87073359 @default.