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- W2801350946 abstract "Abstract Motivation Digital pathology enables new approaches that expand beyond storage, visualization or analysis of histological samples in digital format. One novel opportunity is 3D histology, where a three-dimensional reconstruction of the sample is formed computationally based on serial tissue sections. This allows examining tissue architecture in 3D, for example, for diagnostic purposes. Importantly, 3D histology enables joint mapping of cellular morphology with spatially resolved omics data in the true 3D context of the tissue at microscopic resolution. Several algorithms have been proposed for the reconstruction task, but a quantitative comparison of their accuracy is lacking. Results We developed a benchmarking framework to evaluate the accuracy of several free and commercial 3D reconstruction methods using two whole slide image datasets. The results provide a solid basis for further development and application of 3D histology algorithms and indicate that methods capable of compensating for local tissue deformation are superior to simpler approaches. Availability and implementation Code: https://github.com/BioimageInformaticsTampere/RegBenchmark. Whole slide image datasets: http://urn.fi/urn: nbn: fi: csc-kata20170705131652639702. Supplementary information Supplementary data are available at Bioinformatics online." @default.
- W2801350946 created "2018-05-17" @default.
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- W2801350946 date "2018-04-19" @default.
- W2801350946 modified "2023-10-02" @default.
- W2801350946 title "Comparative analysis of tissue reconstruction algorithms for 3D histology" @default.
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- W2801350946 doi "https://doi.org/10.1093/bioinformatics/bty210" @default.
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