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- W2801385274 abstract "A novel fluorescent probe DNBS-CSA is developed for light-up detection of β-lactamase. The probe design is based on an indirect detection approach with three step reactions. β-Lactamase can react with the lactam of its substrate (cefazolin sodium) to produce a secondary amine, initiating a spontaneous elimination reaction and affording a thiol compound. The thiol could further react with the sulfonate group of DNBS-CSA, releasing the salicylaldehyde azine derivative (CSA) with both aggregation induced emission (AIE) and excited-state intramolecular proton transfer (ESIPT) characteristics. Previously reported β-lactamase probes require covalent linkage of the substrate β-lactam ring part to the probe, which makes probe synthesis difficult due to the complicated structure of the β-lactam ring. In contrast, modification of the β-lactam ring is no longer necessary for DNBS-CSA according to our indirect detection approach. The linear range of fluorescence quantification for β-lactamase is 0-10 mU mL-1 in an aqueous solution. Moreover, owing to the AIE properties of CSA, detection of β-lactamase with DNBS-CSA on test papers was also achieved." @default.
- W2801385274 created "2018-05-17" @default.
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- W2801385274 date "2018-01-01" @default.
- W2801385274 modified "2023-10-16" @default.
- W2801385274 title "A simple design of fluorescent probes for indirect detection of β-lactamase based on AIE and ESIPT processes" @default.
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- W2801385274 doi "https://doi.org/10.1039/c8tb00414e" @default.
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