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• W2801448489 endingPage "e1007028" @default.
• W2801448489 startingPage "e1007028" @default.
• W2801448489 abstract "Positive-strand RNA viruses assemble numerous membrane-bound viral replicase complexes within large replication compartments to support their replication in infected cells. Yet the detailed mechanism of how given subcellular compartments are subverted by viruses is incompletely understood. Although, Tomato bushy stunt virus (TBSV) uses peroxisomal membranes for replication, in this paper, we show evidence that the ER-resident SNARE (soluble NSF attachment protein receptor) proteins play critical roles in the formation of active replicase complexes in yeast model host and in plants. Depletion of the syntaxin 18-like Ufe1 and Use1, which are components of the ER SNARE complex in the ERAS (ER arrival site) subdomain, in yeast resulted in greatly reduced tombusvirus accumulation. Over-expression of a dominant-negative mutant of either the yeast Ufe1 or the orthologous plant Syp81 syntaxin greatly interferes with tombusvirus replication in yeast and plants, thus further supporting the role of this host protein in tombusvirus replication. Moreover, tombusvirus RNA replication was low in cell-free extracts from yeast with repressed Ufe1 or Use1 expression. We also present evidence for the mislocalization of the tombusviral p33 replication protein to the ER membrane in Ufe1p-depleted yeast cells. The viral p33 replication protein interacts with both Ufe1p and Use1p and co-opts them into the TBSV replication compartment in yeast and plant cells. The co-opted Ufe1 affects the virus-driven membrane contact site formation, sterol-enrichment at replication sites, recruitment of several pro-viral host factors and subversion of the Rab5-positive PE-rich endosomes needed for robust TBSV replication. In summary, we demonstrate a critical role for Ufe1 and Use1 SNARE proteins in TBSV replication and propose that the pro-viral functions of Ufe1 and Use1 are to serve as assembly hubs for the formation of the extensive TBSV replication compartments in cells. Altogether, these findings point clearly at the ERAS subdomain of ER as a critical site for the biogenesis of the TBSV replication compartment." @default.
• W2801448489 created "2018-05-17" @default.
• W2801448489 creator A5017052813 @default.
• W2801448489 creator A5017591338 @default.
• W2801448489 creator A5025661624 @default.
• W2801448489 creator A5087992952 @default.
• W2801448489 creator A5090380640 @default.
• W2801448489 date "2018-05-10" @default.
• W2801448489 modified "2023-10-17" @default.
• W2801448489 title "Assembly-hub function of ER-localized SNARE proteins in biogenesis of tombusvirus replication compartment" @default.
• W2801448489 cites W1536725506 @default.
• W2801448489 cites W1561513225 @default.
• W2801448489 cites W1575798288 @default.
• W2801448489 cites W1585380514 @default.
• W2801448489 cites W1596664454 @default.
• W2801448489 cites W1880359614 @default.
• W2801448489 cites W1955443580 @default.
• W2801448489 cites W1974220691 @default.
• W2801448489 cites W1979558190 @default.
• W2801448489 cites W1981671540 @default.
• W2801448489 cites W1986498323 @default.
• W2801448489 cites W1990571611 @default.
• W2801448489 cites W1990725668 @default.
• W2801448489 cites W1991400645 @default.
• W2801448489 cites W1995668581 @default.
• W2801448489 cites W2007745288 @default.
• W2801448489 cites W2012614643 @default.
• W2801448489 cites W2012885662 @default.
• W2801448489 cites W2013705635 @default.
• W2801448489 cites W2024080631 @default.
• W2801448489 cites W2027129957 @default.
• W2801448489 cites W2037423297 @default.
• W2801448489 cites W2038743622 @default.
• W2801448489 cites W2043101137 @default.
• W2801448489 cites W2043312499 @default.
• W2801448489 cites W2059854128 @default.
• W2801448489 cites W2060078754 @default.
• W2801448489 cites W2060726259 @default.
• W2801448489 cites W2064627646 @default.
• W2801448489 cites W2074422749 @default.
• W2801448489 cites W2075833662 @default.
• W2801448489 cites W2077856929 @default.
• W2801448489 cites W2080409718 @default.
• W2801448489 cites W2083901349 @default.
• W2801448489 cites W2086907495 @default.
• W2801448489 cites W2088098522 @default.
• W2801448489 cites W2089400774 @default.
• W2801448489 cites W2089521588 @default.
• W2801448489 cites W2091429085 @default.
• W2801448489 cites W2093020484 @default.
• W2801448489 cites W2096133555 @default.
• W2801448489 cites W2097686812 @default.
• W2801448489 cites W2105728074 @default.
• W2801448489 cites W2107113045 @default.
• W2801448489 cites W2107480012 @default.
• W2801448489 cites W2109187906 @default.
• W2801448489 cites W2117775313 @default.
• W2801448489 cites W2118751686 @default.
• W2801448489 cites W2119477250 @default.
• W2801448489 cites W2120340717 @default.
• W2801448489 cites W2120848963 @default.
• W2801448489 cites W2122006573 @default.
• W2801448489 cites W2122836610 @default.
• W2801448489 cites W2123799993 @default.
• W2801448489 cites W2127825952 @default.
• W2801448489 cites W2133778192 @default.
• W2801448489 cites W2137009674 @default.
• W2801448489 cites W2141132246 @default.
• W2801448489 cites W2143210808 @default.
• W2801448489 cites W2143842613 @default.
• W2801448489 cites W2153515433 @default.
• W2801448489 cites W2154046032 @default.
• W2801448489 cites W2154427656 @default.
• W2801448489 cites W2169394928 @default.
• W2801448489 cites W2184246636 @default.
• W2801448489 cites W2252434158 @default.
• W2801448489 cites W2271324282 @default.
• W2801448489 cites W2271346443 @default.
• W2801448489 cites W2300523604 @default.
• W2801448489 cites W2316450091 @default.
• W2801448489 cites W2337821328 @default.
• W2801448489 cites W2343315663 @default.
• W2801448489 cites W2397285467 @default.
• W2801448489 cites W2399152415 @default.
• W2801448489 cites W2414778334 @default.
• W2801448489 cites W2498709315 @default.
• W2801448489 cites W2512759266 @default.
• W2801448489 cites W2513814202 @default.
• W2801448489 cites W2533211970 @default.
• W2801448489 cites W2546391660 @default.
• W2801448489 cites W2578709657 @default.
• W2801448489 cites W2785234547 @default.
• W2801448489 cites W2953344829 @default.
• W2801448489 doi "https://doi.org/10.1371/journal.ppat.1007028" @default.
• W2801448489 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5963807" @default.
• W2801448489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29746582" @default.
• W2801448489 hasPublicationYear "2018" @default.
• W2801448489 type Work @default.

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