Matches in SemOpenAlex for { <https://semopenalex.org/work/W2801494821> ?p ?o ?g. }
- W2801494821 endingPage "571" @default.
- W2801494821 startingPage "563" @default.
- W2801494821 abstract "Iminodiacetic acid (IDA) is widely used as an intermediate in the manufacturing of chelating agents, glyphosate herbicides and surfactants. To improve activity and tolerance to the substrate for IDA production, Acidovorax facilis nitrilase was selected for further modification by the gene site saturation mutagenesis method. After screened by a two-step screening method, the best mutant (Mut-F168V/T201N/S192F/M191T/F192S) was selected. Compared to the wild-type nitrilase, Mut-F168V/T201N/S192F/M191T/F192S showed 136% improvement in specific activity. Co2+ stimulated nitrilase activity, whereas Cu2+, Zn2+ and Tween 80 showed a strong inhibitory effect. The Vmax and kcat of Mut-F168V/T201N/S192F/M191T/F192S were enhanced 1.23 and 1.23-fold, while the Km was decreased 1.53-fold. The yield of Mut-F168V/T201N/S192F/M191T/F192S with 453.2 mM of IDA reached 71.9% in 5 h when 630 mM iminodiacetonitrile was used as substrate. This study indicated that mutant nitrilase obtained in this study is promising in applications for the upscale production of IDAN." @default.
- W2801494821 created "2018-05-17" @default.
- W2801494821 creator A5013745189 @default.
- W2801494821 creator A5015292967 @default.
- W2801494821 creator A5048171321 @default.
- W2801494821 creator A5049584538 @default.
- W2801494821 creator A5050323272 @default.
- W2801494821 creator A5058146813 @default.
- W2801494821 creator A5064014860 @default.
- W2801494821 creator A5086792289 @default.
- W2801494821 creator A5089034091 @default.
- W2801494821 date "2018-09-01" @default.
- W2801494821 modified "2023-10-12" @default.
- W2801494821 title "Significant improvement of the nitrilase activity by semi-rational protein engineering and its application in the production of iminodiacetic acid" @default.
- W2801494821 cites W1491401240 @default.
- W2801494821 cites W1652052270 @default.
- W2801494821 cites W1809713791 @default.
- W2801494821 cites W1967260057 @default.
- W2801494821 cites W1975920595 @default.
- W2801494821 cites W1991395609 @default.
- W2801494821 cites W1997954108 @default.
- W2801494821 cites W2003095680 @default.
- W2801494821 cites W2006200738 @default.
- W2801494821 cites W2010618393 @default.
- W2801494821 cites W2024638068 @default.
- W2801494821 cites W2025938842 @default.
- W2801494821 cites W2026894454 @default.
- W2801494821 cites W2031490903 @default.
- W2801494821 cites W2037030327 @default.
- W2801494821 cites W2037054178 @default.
- W2801494821 cites W2047549055 @default.
- W2801494821 cites W2048846306 @default.
- W2801494821 cites W2052895657 @default.
- W2801494821 cites W2054172648 @default.
- W2801494821 cites W2057331258 @default.
- W2801494821 cites W2076276322 @default.
- W2801494821 cites W2094694540 @default.
- W2801494821 cites W2105371545 @default.
- W2801494821 cites W2110282379 @default.
- W2801494821 cites W2158534713 @default.
- W2801494821 cites W2324384628 @default.
- W2801494821 cites W2326821812 @default.
- W2801494821 cites W2567812982 @default.
- W2801494821 cites W2571341234 @default.
- W2801494821 cites W2606101851 @default.
- W2801494821 cites W2612158793 @default.
- W2801494821 cites W2729944258 @default.
- W2801494821 cites W2744248965 @default.
- W2801494821 cites W2762477575 @default.
- W2801494821 doi "https://doi.org/10.1016/j.ijbiomac.2018.05.045" @default.
- W2801494821 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29753012" @default.
- W2801494821 hasPublicationYear "2018" @default.
- W2801494821 type Work @default.
- W2801494821 sameAs 2801494821 @default.
- W2801494821 citedByCount "36" @default.
- W2801494821 countsByYear W28014948212018 @default.
- W2801494821 countsByYear W28014948212019 @default.
- W2801494821 countsByYear W28014948212020 @default.
- W2801494821 countsByYear W28014948212021 @default.
- W2801494821 countsByYear W28014948212022 @default.
- W2801494821 countsByYear W28014948212023 @default.
- W2801494821 crossrefType "journal-article" @default.
- W2801494821 hasAuthorship W2801494821A5013745189 @default.
- W2801494821 hasAuthorship W2801494821A5015292967 @default.
- W2801494821 hasAuthorship W2801494821A5048171321 @default.
- W2801494821 hasAuthorship W2801494821A5049584538 @default.
- W2801494821 hasAuthorship W2801494821A5050323272 @default.
- W2801494821 hasAuthorship W2801494821A5058146813 @default.
- W2801494821 hasAuthorship W2801494821A5064014860 @default.
- W2801494821 hasAuthorship W2801494821A5086792289 @default.
- W2801494821 hasAuthorship W2801494821A5089034091 @default.
- W2801494821 hasConcept C104317684 @default.
- W2801494821 hasConcept C119599485 @default.
- W2801494821 hasConcept C127413603 @default.
- W2801494821 hasConcept C143065580 @default.
- W2801494821 hasConcept C178790620 @default.
- W2801494821 hasConcept C181199279 @default.
- W2801494821 hasConcept C185592680 @default.
- W2801494821 hasConcept C197404232 @default.
- W2801494821 hasConcept C2776865271 @default.
- W2801494821 hasConcept C2780296214 @default.
- W2801494821 hasConcept C2781137352 @default.
- W2801494821 hasConcept C41183919 @default.
- W2801494821 hasConcept C55493867 @default.
- W2801494821 hasConcept C56856141 @default.
- W2801494821 hasConceptScore W2801494821C104317684 @default.
- W2801494821 hasConceptScore W2801494821C119599485 @default.
- W2801494821 hasConceptScore W2801494821C127413603 @default.
- W2801494821 hasConceptScore W2801494821C143065580 @default.
- W2801494821 hasConceptScore W2801494821C178790620 @default.
- W2801494821 hasConceptScore W2801494821C181199279 @default.
- W2801494821 hasConceptScore W2801494821C185592680 @default.
- W2801494821 hasConceptScore W2801494821C197404232 @default.
- W2801494821 hasConceptScore W2801494821C2776865271 @default.
- W2801494821 hasConceptScore W2801494821C2780296214 @default.
- W2801494821 hasConceptScore W2801494821C2781137352 @default.
- W2801494821 hasConceptScore W2801494821C41183919 @default.
- W2801494821 hasConceptScore W2801494821C55493867 @default.