Matches in SemOpenAlex for { <https://semopenalex.org/work/W2801508959> ?p ?o ?g. }
- W2801508959 endingPage "42" @default.
- W2801508959 startingPage "42" @default.
- W2801508959 abstract "Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins. In particular, for thermostable proteins, there is a need for hosts able to properly synthesize, fold, and excrete these in high yields, and thermophilic Bacillaceae represent one potentially interesting group of microorganisms for such purposes. A number of thermophilic Bacillaceae including B. methanolicus, B. coagulans, B. smithii, B. licheniformis, Geobacillus thermoglucosidasius, G. kaustophilus, and G. stearothermophilus are investigated concerning physiology, genomics, genetic tools, and technologies, altogether paving the way for their utilization as hosts for recombinant production of thermostable and other difficult-to-express proteins. Moreover, recent successful deployments of CRISPR/Cas9 in several of these species have accelerated the progress in their metabolic engineering, which should increase their attractiveness for future industrial-scale production of proteins. This review describes the biology of thermophilic Bacillaceae and in particular focuses on genetic tools and methods enabling use of these organisms as hosts for recombinant protein production." @default.
- W2801508959 created "2018-05-17" @default.
- W2801508959 creator A5007163296 @default.
- W2801508959 creator A5035665980 @default.
- W2801508959 creator A5047303066 @default.
- W2801508959 creator A5068958346 @default.
- W2801508959 date "2018-05-10" @default.
- W2801508959 modified "2023-10-05" @default.
- W2801508959 title "Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae" @default.
- W2801508959 cites W117822466 @default.
- W2801508959 cites W1277850551 @default.
- W2801508959 cites W1485776054 @default.
- W2801508959 cites W1501688989 @default.
- W2801508959 cites W1504465245 @default.
- W2801508959 cites W1506032860 @default.
- W2801508959 cites W1559169404 @default.
- W2801508959 cites W1572472556 @default.
- W2801508959 cites W1592204725 @default.
- W2801508959 cites W1638949336 @default.
- W2801508959 cites W1755403693 @default.
- W2801508959 cites W1771799254 @default.
- W2801508959 cites W1795879484 @default.
- W2801508959 cites W1802247160 @default.
- W2801508959 cites W1805287899 @default.
- W2801508959 cites W1838414788 @default.
- W2801508959 cites W1907206755 @default.
- W2801508959 cites W1956487875 @default.
- W2801508959 cites W1963998099 @default.
- W2801508959 cites W1967855250 @default.
- W2801508959 cites W1974000166 @default.
- W2801508959 cites W1975156465 @default.
- W2801508959 cites W1975835387 @default.
- W2801508959 cites W1978584284 @default.
- W2801508959 cites W1980724032 @default.
- W2801508959 cites W1981448996 @default.
- W2801508959 cites W1981563281 @default.
- W2801508959 cites W1981925964 @default.
- W2801508959 cites W1983086586 @default.
- W2801508959 cites W1984115296 @default.
- W2801508959 cites W1985550071 @default.
- W2801508959 cites W1986775087 @default.
- W2801508959 cites W1987062982 @default.
- W2801508959 cites W1991303432 @default.
- W2801508959 cites W1993924598 @default.
- W2801508959 cites W1995892691 @default.
- W2801508959 cites W1998427361 @default.
- W2801508959 cites W2000668033 @default.
- W2801508959 cites W2006707090 @default.
- W2801508959 cites W2007389794 @default.
- W2801508959 cites W2010400197 @default.
- W2801508959 cites W2011851607 @default.
- W2801508959 cites W2012440402 @default.
- W2801508959 cites W2016359194 @default.
- W2801508959 cites W2017832416 @default.
- W2801508959 cites W2021548280 @default.
- W2801508959 cites W2025545461 @default.
- W2801508959 cites W2026328870 @default.
- W2801508959 cites W2028005056 @default.
- W2801508959 cites W2028341858 @default.
- W2801508959 cites W2028819914 @default.
- W2801508959 cites W2029405823 @default.
- W2801508959 cites W2032408161 @default.
- W2801508959 cites W2032706307 @default.
- W2801508959 cites W2034869114 @default.
- W2801508959 cites W2035490837 @default.
- W2801508959 cites W2038913723 @default.
- W2801508959 cites W2049955359 @default.
- W2801508959 cites W2051258924 @default.
- W2801508959 cites W2055307142 @default.
- W2801508959 cites W2057416949 @default.
- W2801508959 cites W2059329766 @default.
- W2801508959 cites W2061104342 @default.
- W2801508959 cites W2062294464 @default.
- W2801508959 cites W2065592668 @default.
- W2801508959 cites W2068437670 @default.
- W2801508959 cites W2070380721 @default.
- W2801508959 cites W2074386261 @default.
- W2801508959 cites W2089433978 @default.
- W2801508959 cites W2096217709 @default.
- W2801508959 cites W2100662644 @default.
- W2801508959 cites W2101164711 @default.
- W2801508959 cites W2102363627 @default.
- W2801508959 cites W2102838603 @default.
- W2801508959 cites W2105384650 @default.
- W2801508959 cites W2106078529 @default.
- W2801508959 cites W2106450869 @default.
- W2801508959 cites W2107325876 @default.
- W2801508959 cites W2108268803 @default.
- W2801508959 cites W2113230913 @default.
- W2801508959 cites W2115588671 @default.
- W2801508959 cites W2116479274 @default.
- W2801508959 cites W2116835520 @default.
- W2801508959 cites W2120353169 @default.
- W2801508959 cites W2120503144 @default.
- W2801508959 cites W2121612894 @default.
- W2801508959 cites W2121677860 @default.
- W2801508959 cites W2122511648 @default.
- W2801508959 cites W2126905359 @default.