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• W2801683598 abstract "Met80, one of the heme iron ligands in cytochrome c (cyt c), is readily oxidized to Met sulfoxide (Met-SO) by several biologically relevant oxidants. The modification has been suggested to affect both the electron-transfer (ET) and apoptotic functions of this metalloprotein. The coordination of the heme iron in Met-oxidized cyt c (Met-SO cyt c) is critical for both of these functions but has remained poorly defined. We present electronic absorption, NMR, and EPR spectroscopic investigations as well as kinetic studies and mutational analyses to identify the heme iron ligands in yeast iso-1 Met-SO cyt c. Similar to the alkaline form of native cyt c, Lys73 and Lys79 ligate to the ferric heme iron in the Met80-oxidized protein, but this coordination takes place at much lower pH. The ferrous heme iron is ligated by Met-SO, implying the redox-linked ligand switch in the modified protein. Binding studies with the model peptide microperoxidase-8 provide a rationale for alterations in ligation and for the role of the polypeptide packing in native and Met-SO cyt c. Imidazole binding experiments have revealed that Lys dissociation from the ferric heme in K73A/K79G/M80K (M80K#) and Met-SO is more than 3 orders of magnitude slower than the opening of the heme pocket that limits Met80 replacement in native cyt c. The Lys-to-Met-SO ligand substitution gates ET of ferric Met-SO cyt c with Co(terpy)22+. Owing to the slow Lys dissociation step, ET reaction is slow but possible, which is not the case for nonswitchable M80A and M80K#. Acidic conditions cause Lys replacement by a water ligand in Met-SO cyt c (p Ka = 6.3 ± 0.1), increasing the intrinsic peroxidase activity of the protein. This pH-driven ligand switch may be a mechanism to boost peroxidase function of cyt c specifically in apoptotic cells." @default.
• W2801683598 created "2018-05-17" @default.
• W2801683598 creator A5036703473 @default.
• W2801683598 creator A5065747383 @default.
• W2801683598 date "2018-04-30" @default.
• W2801683598 modified "2023-09-29" @default.
• W2801683598 title "Ligation and Reactivity of Methionine-Oxidized Cytochrome c" @default.
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• W2801683598 cites W1965787155 @default.
• W2801683598 cites W1971915692 @default.
• W2801683598 cites W1973122103 @default.
• W2801683598 cites W1976008991 @default.
• W2801683598 cites W1978072574 @default.
• W2801683598 cites W1983276480 @default.
• W2801683598 cites W1983325222 @default.
• W2801683598 cites W1988920308 @default.
• W2801683598 cites W1990204266 @default.
• W2801683598 cites W1990512618 @default.
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• W2801683598 cites W2029667189 @default.
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• W2801683598 cites W2040890941 @default.
• W2801683598 cites W2042207251 @default.
• W2801683598 cites W2042778264 @default.
• W2801683598 cites W2047039535 @default.
• W2801683598 cites W2049774523 @default.
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• W2801683598 cites W2069286623 @default.
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• W2801683598 cites W2072441592 @default.
• W2801683598 cites W2078803097 @default.
• W2801683598 cites W2080780045 @default.
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• W2801683598 doi "https://doi.org/10.1021/acs.inorgchem.8b00010" @default.
• W2801683598 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6107088" @default.
• W2801683598 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29708337" @default.
• W2801683598 hasPublicationYear "2018" @default.
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