FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2801686500> ?p ?o ?g. }

• W2801686500 endingPage "S129" @default.
• W2801686500 startingPage "S129" @default.
• W2801686500 abstract "Purpose: Osteoarthritis (OA) is one of the most prevalent diseases in the elderly with high economic burden for health care systems worldwide. Pathophysiology is characterized by degradation of extracellular matrix components (ECM), eventually leading to progressive loss of cartilage and bone. Therapeutic strategies are limited to treating major symptoms during onset and progression of the disease with joint arthroplasty being the ultimate treatment in end-stage OA. Approaches to halt disease progression are not existent. In preliminary studies we demonstrated an influence of T cells on MMP/ADAMTS secretion. The aim of this project was to quantitatively analyze the influence of pro-/anti-inflammatory macrophage phenotype (M1/M2) on enzymatic, chondrodestructive processes in a direct co-culture. Methods: During arthroplastic surgery SM, chondrocytes (CHO) and peripheral blood (PB) where obtained from a total of 28 patients (age: 65.9 ± 11.1 yrs; 16m, 12f) with moderate to severe osteoarthritis of the knee (Kellgren & Lawrence grade II-IV). CHO were isolated from cartilage by enzymatic. Subsequently CHO were filtered and taken into cell culture for 9 days. Simultaneously autologous mononuclear cells (MNC) were isolated from PB via density gradient centrifugation. After centrifugation, MNC were depleted and native macrophages were differentiated into pro-inflammatory M1 (CD14+CD80+HLA-DR+) or anti-inflammatory M2 (CD14+CD163+CD206+), for 9 days according to manufacturer's protocol (Promocell, Germany). Purity of macrophage differentiation was confirmed by flow cytometry. CHO/M1 and CHO/M2 co-cultures (adding 10ng/ml GM-CSF, resp. 10ng/ml M-CSF) were further cultivated for 5 more days. As a control CHO and M1/M2 monocultures were cultivated in the same medium. The continued macrophage differentiation was once again controlled by flow cytometry after 5 days. The concentration of chondro-destructive MMP-1/-3/-9; ADAMTS-5 in each mono- or co-culture was measured with ELISA after the final time of incubation. Furthermore, MNC were isolated from SM by density gradient centrifugation, magnetic cell sorting and enzymatic digestion. The relative amount of pro- and anti-inflammatory macrophages was determined by flow cytometry: M1 (CD14+CD80+HLA-DR+); M2 (CD14+CD163+CD206+). Results: After 5 days of incubation in direct cell culture with autologous chondrocytes, M1/M2 widely obtained their phenotype, as proven by M1/M2 purity of 90±2.5%, resp. 87.3±3.0% at day 5 compared to 98.2±1.2%/81.2±2.4 at day 0. Both M1 and M2 generated a highly significant induction of MMP-1/-3 and ADAMTS-5-secretion in chondrocyte co-culture compared to chondrocyte and macrophage-monocultures, with M2 having the significantly strongest effect on MMP1 and ADAMTS5, whereas M1 enhanced MMP3-secretion significantly most. The highest values for MMP9 were measured in macrophage monocultures. Conclusions: This is to our knowledge the first experiment to achieve stable macrophage phenotypes for the complete cell culture duration. We show here, that M2 as well as M1 macrophages increase enzymatic secretion in co-culture with chondrocytes significantly. Our data suggests that macrophages play an important role in the process of enzymatic cartilage degradation during disease progression, although our data neither clearly indicates which phenotype has the predominant influence, nor if M1/M2 act on different pathways. We show here that macrophages act extremely versatile and can hardly be squeezed into the conventional pro-/anti-inflammatory pattern." @default.
• W2801686500 created "2018-05-17" @default.
• W2801686500 creator A5000541602 @default.
• W2801686500 creator A5033557216 @default.
• W2801686500 creator A5037255119 @default.
• W2801686500 creator A5069799428 @default.
• W2801686500 creator A5080165844 @default.
• W2801686500 date "2018-04-01" @default.
• W2801686500 modified "2023-10-14" @default.
• W2801686500 title "M1/M2 macrophages induce chondral MMP/ADAMTS enzyme secretion in a direct co-culture experiment" @default.
• W2801686500 doi "https://doi.org/10.1016/j.joca.2018.02.280" @default.
• W2801686500 hasPublicationYear "2018" @default.
• W2801686500 type Work @default.
• W2801686500 sameAs 2801686500 @default.
• W2801686500 citedByCount "3" @default.
• W2801686500 countsByYear W28016865002018 @default.
• W2801686500 countsByYear W28016865002019 @default.
• W2801686500 crossrefType "journal-article" @default.
• W2801686500 hasAuthorship W2801686500A5000541602 @default.
• W2801686500 hasAuthorship W2801686500A5033557216 @default.
• W2801686500 hasAuthorship W2801686500A5037255119 @default.
• W2801686500 hasAuthorship W2801686500A5069799428 @default.
• W2801686500 hasAuthorship W2801686500A5080165844 @default.
• W2801686500 hasBestOaLocation W28016865001 @default.
• W2801686500 hasConcept C109523444 @default.
• W2801686500 hasConcept C126322002 @default.
• W2801686500 hasConcept C137061746 @default.
• W2801686500 hasConcept C142724271 @default.
• W2801686500 hasConcept C1491633281 @default.
• W2801686500 hasConcept C16685009 @default.
• W2801686500 hasConcept C167814343 @default.
• W2801686500 hasConcept C16930146 @default.
• W2801686500 hasConcept C185592680 @default.
• W2801686500 hasConcept C202751555 @default.
• W2801686500 hasConcept C203014093 @default.
• W2801686500 hasConcept C204787440 @default.
• W2801686500 hasConcept C2775858924 @default.
• W2801686500 hasConcept C2776164576 @default.
• W2801686500 hasConcept C2776224515 @default.
• W2801686500 hasConcept C2778513237 @default.
• W2801686500 hasConcept C2779244956 @default.
• W2801686500 hasConcept C2780007613 @default.
• W2801686500 hasConcept C2780932548 @default.
• W2801686500 hasConcept C553184892 @default.
• W2801686500 hasConcept C55493867 @default.
• W2801686500 hasConcept C55728118 @default.
• W2801686500 hasConcept C71924100 @default.
• W2801686500 hasConceptScore W2801686500C109523444 @default.
• W2801686500 hasConceptScore W2801686500C126322002 @default.
• W2801686500 hasConceptScore W2801686500C137061746 @default.
• W2801686500 hasConceptScore W2801686500C142724271 @default.
• W2801686500 hasConceptScore W2801686500C1491633281 @default.
• W2801686500 hasConceptScore W2801686500C16685009 @default.
• W2801686500 hasConceptScore W2801686500C167814343 @default.
• W2801686500 hasConceptScore W2801686500C16930146 @default.
• W2801686500 hasConceptScore W2801686500C185592680 @default.
• W2801686500 hasConceptScore W2801686500C202751555 @default.
• W2801686500 hasConceptScore W2801686500C203014093 @default.
• W2801686500 hasConceptScore W2801686500C204787440 @default.
• W2801686500 hasConceptScore W2801686500C2775858924 @default.
• W2801686500 hasConceptScore W2801686500C2776164576 @default.
• W2801686500 hasConceptScore W2801686500C2776224515 @default.
• W2801686500 hasConceptScore W2801686500C2778513237 @default.
• W2801686500 hasConceptScore W2801686500C2779244956 @default.
• W2801686500 hasConceptScore W2801686500C2780007613 @default.
• W2801686500 hasConceptScore W2801686500C2780932548 @default.
• W2801686500 hasConceptScore W2801686500C553184892 @default.
• W2801686500 hasConceptScore W2801686500C55493867 @default.
• W2801686500 hasConceptScore W2801686500C55728118 @default.
• W2801686500 hasConceptScore W2801686500C71924100 @default.
• W2801686500 hasLocation W28016865001 @default.
• W2801686500 hasOpenAccess W2801686500 @default.
• W2801686500 hasPrimaryLocation W28016865001 @default.
• W2801686500 hasRelatedWork W2038751394 @default.
• W2801686500 hasRelatedWork W2048583725 @default.
• W2801686500 hasRelatedWork W2055797436 @default.
• W2801686500 hasRelatedWork W2156393353 @default.
• W2801686500 hasRelatedWork W2353278676 @default.
• W2801686500 hasRelatedWork W3164853693 @default.
• W2801686500 hasRelatedWork W3200600991 @default.
• W2801686500 hasRelatedWork W3216725015 @default.
• W2801686500 hasRelatedWork W390244266 @default.
• W2801686500 hasRelatedWork W2469134581 @default.
• W2801686500 hasVolume "26" @default.
• W2801686500 isParatext "false" @default.
• W2801686500 isRetracted "false" @default.
• W2801686500 magId "2801686500" @default.
• W2801686500 workType "article" @default.