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- W2801694687 abstract "Thiss study was conducted to investigate the possible role of a compatible solute from radio‐halophilic bacterium against desiccation and ultra‐violet radiation‐induced oxidative stress. Nine different radio‐resistant bacteria were isolated from desert soil, where strain WMA‐LM19 was chosen for detailed studies on the basis of its high tolerance to ultraviolet radiation among all these isolates. Here, 16S rRNA gene sequencing indicated the bacterium was closely related to Stenotrophomonas sp. (KT008383). A bacterial milking strategy was applied for extraction of intracellular compatible solutes in 70% (v/v) ethanol, which were purified by High Performance Liquid Chromatography (HPLC). The compound was characterized as ectoine by 1H and 13C Nuclear Magnetic Resonance (NMR), and Mass Spectrometry (MS). Ectoine inhibited oxidative damage to proteins and lipids in comparison to the standard ascorbic acid. It also demonstrated more efficient prevention (54·80%) against lysis to erythrocytes membrane by surface active agents than lecithin. Furthermore, a high level of ectoine‐mediated protection of bovine serum albumin against ionizing radiation (1 500–2 000Jm−2) was observed, as indicated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE) analysis. The results indicated that ectoine from Stenotrophomonas sp. WMA‐LM19 can be used as a potential mitigator and radio‐protective agent to overcome radiation‐ and salinity‐mediated oxidative damages in extreme environment. Due to its anti‐oxidant properties, ectoine from a radio‐halophilic bacterium might be used in sunscreen formulation for protection against UV‐induced oxidative stress." @default.
- W2801694687 created "2018-05-17" @default.
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- W2801694687 date "2018-06-06" @default.
- W2801694687 modified "2023-09-26" @default.
- W2801694687 title "Ectoine: a compatible solute in radio‐halophilic<i>Stenotrophomonas</i>sp.<scp>WMA</scp>‐<scp>LM</scp>19 strain to prevent ultraviolet‐induced protein damage" @default.
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- W2801694687 doi "https://doi.org/10.1111/jam.13903" @default.
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