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• W2801792013 abstract "We examined the effect of fucoidan, an immune modulator, on the phagocytic capacity of porcine peripheral blood polymorphonuclear cells (PMNs) exposed to culture supernatant from lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs). For this purpose, we evaluated the phagocytic capacity of porcine PMNs by flow cytometry and measured levels of tumor necrosis factor-alpha (TNF-α) protein and mRNA in porcine PBMCs by enzyme-linked immunosorbent assay (ELISA) and real time-polymerase chain reaction (PCR), respectively. Fucoidan or LPS alone did not affect the phagocytic capacity of PMNs, but phagocytosis by these cells was increased by exposure to culture supernatant from PBMCs treated with fucoidan or LPS. In particular, the culture supernatant from PBMCs treated with LPS revealed excessive phagocytosis of PMNs. This excessive phagocytic capacity was diminished by co-treatment LPS with fucoidan. Production of TNF-α mRNA and protein increased upon treatment of PBMCs with either fucoidan or LPS, but this effect was also diminished by co-treatment LPS with fucoidan. The ability of culture supernatant from PBMCs treated with LPS and/or fucoidan to increase the phagocytic capacity of PMNs was inhibited by anti-recombinant porcine TNF-α polyclonal antibody. These results suggested that fucoidan suppresses the phagocytic capacity of PMNs by modulating TNF-α production by LPS-stimulated PBMCs." @default.
• W2801792013 created "2018-05-17" @default.
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• W2801792013 date "2018-06-01" @default.
• W2801792013 modified "2023-10-01" @default.
• W2801792013 title "Fucoidan suppresses excessive phagocytic capacity of porcine peripheral blood polymorphonuclear cells by modulating production of tumor necrosis factor-alpha by lipopolysaccharide-stimulated peripheral blood mononuclear cells" @default.
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• W2801792013 doi "https://doi.org/10.1016/j.rvsc.2018.04.007" @default.
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