FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2801794631> ?p ?o ?g. }

• W2801794631 endingPage "32" @default.
• W2801794631 startingPage "22" @default.
• W2801794631 abstract "Abstract The Junonia coenia densovirus rapidly traverses the gut epithelium of the host lepidopteran without replicating in the gut cells. The ability of this virus to transcytose across the gut epithelium is of interest for the potential use of virus structural proteins as delivery vehicles for insecticidal peptides that act within the insect hemocoel, rather than in the gut. In this study, we used fall armyworm, Spodoptera frugiperda to examine the binding of the virus to brush border membrane vesicle proteins by two‐dimensional ligand blot analysis. We also assessed the rate of flux of the primary viral structural protein, VP4 fused to eGFP with a proline‐rich linker (VP4‐P‐eGFP) through the gut epithelium ex vivo in an Ussing chamber. The mechanisms involved with transcytosis of VP4‐P‐eGFP were assessed by use of inhibitors. Bovine serum albumin (BSA) and eGFP were used as positive and negative control proteins, respectively. In contrast to BSA, which binds to multiple proteins on the brush border membrane, VP4‐P‐eGFP binding was specific to a protein of high molecular mass. Protein flux was significantly higher for VP4‐P‐eGFP after 2 h than for albumin or eGFP, with rapid transcytosis of VP4‐P‐eGFP within the first 30 min. In contrast to BSA which transcytosed following clathrin‐mediated endocytosis, the movement of VP4‐P‐eGFP was vesicle‐mediated but clathrin‐independent. The specificity of binding combined with the efficiency of transport across the gut epithelium suggest that VP4 will provide a useful carrier for insecticidal peptides active within the hemocoel of key lepidopteran pests including S. frugiperda ." @default.
• W2801794631 created "2018-05-17" @default.
• W2801794631 creator A5027759561 @default.
• W2801794631 creator A5085742825 @default.
• W2801794631 date "2018-06-03" @default.
• W2801794631 modified "2023-10-01" @default.
• W2801794631 title "Transcytosis of <i>Junonia coenia</i> densovirus VP4 across the gut epithelium of <i>Spodoptera frugiperda</i> (Lepidoptera: Noctuidae)" @default.
• W2801794631 cites W11606465 @default.
• W2801794631 cites W1502026371 @default.
• W2801794631 cites W1526128150 @default.
• W2801794631 cites W1561536970 @default.
• W2801794631 cites W17511328 @default.
• W2801794631 cites W186995127 @default.
• W2801794631 cites W1902182170 @default.
• W2801794631 cites W1965822665 @default.
• W2801794631 cites W1987743187 @default.
• W2801794631 cites W1989745030 @default.
• W2801794631 cites W1999328455 @default.
• W2801794631 cites W2002936944 @default.
• W2801794631 cites W2004659829 @default.
• W2801794631 cites W2004963592 @default.
• W2801794631 cites W2016541541 @default.
• W2801794631 cites W2023604880 @default.
• W2801794631 cites W2036179596 @default.
• W2801794631 cites W2036878863 @default.
• W2801794631 cites W2042558793 @default.
• W2801794631 cites W2055314604 @default.
• W2801794631 cites W205913778 @default.
• W2801794631 cites W2062688272 @default.
• W2801794631 cites W2070947895 @default.
• W2801794631 cites W2080447341 @default.
• W2801794631 cites W2082802628 @default.
• W2801794631 cites W2083052545 @default.
• W2801794631 cites W2084656248 @default.
• W2801794631 cites W2086271092 @default.
• W2801794631 cites W2107825457 @default.
• W2801794631 cites W2110885272 @default.
• W2801794631 cites W2126018089 @default.
• W2801794631 cites W2128073840 @default.
• W2801794631 cites W2146475278 @default.
• W2801794631 cites W2151154068 @default.
• W2801794631 cites W2153516541 @default.
• W2801794631 cites W2154836432 @default.
• W2801794631 cites W2163658976 @default.
• W2801794631 cites W2165970493 @default.
• W2801794631 cites W2168460717 @default.
• W2801794631 cites W2171502209 @default.
• W2801794631 cites W2171984425 @default.
• W2801794631 cites W2266837556 @default.
• W2801794631 cites W2330776218 @default.
• W2801794631 cites W4293247451 @default.
• W2801794631 doi "https://doi.org/10.1111/1744-7917.12600" @default.
• W2801794631 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29704325" @default.
• W2801794631 hasPublicationYear "2018" @default.
• W2801794631 type Work @default.
• W2801794631 sameAs 2801794631 @default.
• W2801794631 citedByCount "5" @default.
• W2801794631 countsByYear W28017946312018 @default.
• W2801794631 countsByYear W28017946312021 @default.
• W2801794631 countsByYear W28017946312022 @default.
• W2801794631 countsByYear W28017946312023 @default.
• W2801794631 crossrefType "journal-article" @default.
• W2801794631 hasAuthorship W2801794631A5027759561 @default.
• W2801794631 hasAuthorship W2801794631A5085742825 @default.
• W2801794631 hasBestOaLocation W28017946312 @default.
• W2801794631 hasConcept C104317684 @default.
• W2801794631 hasConcept C130316041 @default.
• W2801794631 hasConcept C170493617 @default.
• W2801794631 hasConcept C185350488 @default.
• W2801794631 hasConcept C2777773057 @default.
• W2801794631 hasConcept C2781077229 @default.
• W2801794631 hasConcept C28005876 @default.
• W2801794631 hasConcept C40767141 @default.
• W2801794631 hasConcept C41625074 @default.
• W2801794631 hasConcept C529295009 @default.
• W2801794631 hasConcept C5426402 @default.
• W2801794631 hasConcept C54355233 @default.
• W2801794631 hasConcept C55493867 @default.
• W2801794631 hasConcept C63162447 @default.
• W2801794631 hasConcept C86803240 @default.
• W2801794631 hasConcept C95444343 @default.
• W2801794631 hasConceptScore W2801794631C104317684 @default.
• W2801794631 hasConceptScore W2801794631C130316041 @default.
• W2801794631 hasConceptScore W2801794631C170493617 @default.
• W2801794631 hasConceptScore W2801794631C185350488 @default.
• W2801794631 hasConceptScore W2801794631C2777773057 @default.
• W2801794631 hasConceptScore W2801794631C2781077229 @default.
• W2801794631 hasConceptScore W2801794631C28005876 @default.
• W2801794631 hasConceptScore W2801794631C40767141 @default.
• W2801794631 hasConceptScore W2801794631C41625074 @default.
• W2801794631 hasConceptScore W2801794631C529295009 @default.
• W2801794631 hasConceptScore W2801794631C5426402 @default.
• W2801794631 hasConceptScore W2801794631C54355233 @default.
• W2801794631 hasConceptScore W2801794631C55493867 @default.
• W2801794631 hasConceptScore W2801794631C63162447 @default.
• W2801794631 hasConceptScore W2801794631C86803240 @default.
• W2801794631 hasConceptScore W2801794631C95444343 @default.
• W2801794631 hasFunder F4320337396 @default.

• next