FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2801843945> ?p ?o ?g. }

• W2801843945 abstract "HSV-1(KOS)$Delta$gK, a virus lacking the UL53 gene coding for glycoprotein K(gK) was constructed, characterized and compared to the partially deleted gK-null virus HSV-1(F)-gK$beta$. $Delta$gK and F-gK$beta$ viruses produced microscopic plaques on Vero cells at 48 hours post infection. F-gK$beta$ plaques at 72 hours post infection contained fused cells (syn), while $Delta$gK plaques resembled wild-type KOS plaques ($synsp+$). $Delta$gK replicated efficiently in exponentially dividing Vero cells whereas F-gK$beta$ yields were 10 and 1000-fold lower than $Delta$gK yields in HEp-2 or log-phase Vero cells, respectively. Electron micrographs of $Delta$gK-infected Vero cells revealed large numbers of capsids in the nuclei of Vero, but not HEp-2 cells, while a small number of enveloped virions were observed in the cytoplasm of infected Vero and HEp-2 cells that failed to translocate to the extracellular spaces. KOS and $Delta$gK replicated inefficiently in gK-transformed cells, with the exception of VK302 cells. Electron micrographs of the gK-transformed BL-1 cells infected with KOS revealed virions within perinuclear spaces, while there were no virions visualized in $Delta$gK infected BL-1 cells. VK302 cells were the only gK-transformed cells that supported the replication and cellular egress of both KOS and $Delta$gK. To fine-map the functional domains of gK involved in membrane fusion, a long-PCR site-directed mutagenesis system was developed that efficiently produced single base substitutions within larger than 4 kbp DNA fragments. Utilizing this system three different mutations were engineered and recombinant viruses containing one of the three mutations were isolated. The mutation at position 31 resulted in a $synsp+$ virus. This research revealed that gK is involved in nucleocapsid envelopment and cellular egress of infectious virions. Actively replicating cells can partially compensate for envelopment but not for the cellular egress deficiencies of $Delta$gK. Interference of viral replication in gK-transformed cells suggests that the cellular gK can function in-trans to inhibit egress by preventing certain cellular factors required for viral egress to function efficiently. These results implicate that viral egress involves cellular receptors that are modified to act as pilots or sorters for virus transport and egress in a similar fashion to intracellular glycoprotein transport." @default.
• W2801843945 created "2018-05-17" @default.
• W2801843945 creator A5051254783 @default.
• W2801843945 date "2022-06-14" @default.
• W2801843945 modified "2023-10-07" @default.
• W2801843945 title "Genetics and Functions of Herpes Simplex Virus Type 1 (HSV-1) Glycoprotein K (gK) in Virus Envelopment and Egress." @default.
• W2801843945 cites W113864290 @default.
• W2801843945 cites W116397687 @default.
• W2801843945 cites W1489925180 @default.
• W2801843945 cites W1498670035 @default.
• W2801843945 cites W1499615242 @default.
• W2801843945 cites W1501744522 @default.
• W2801843945 cites W1502427113 @default.
• W2801843945 cites W1504349146 @default.
• W2801843945 cites W1504582018 @default.
• W2801843945 cites W1506111130 @default.
• W2801843945 cites W1507428456 @default.
• W2801843945 cites W1510210255 @default.
• W2801843945 cites W1513390311 @default.
• W2801843945 cites W1513508804 @default.
• W2801843945 cites W1517483121 @default.
• W2801843945 cites W1521918462 @default.
• W2801843945 cites W1523114181 @default.
• W2801843945 cites W1526548004 @default.
• W2801843945 cites W1530359565 @default.
• W2801843945 cites W1532124527 @default.
• W2801843945 cites W1533896708 @default.
• W2801843945 cites W1541438815 @default.
• W2801843945 cites W1544957784 @default.
• W2801843945 cites W1547083610 @default.
• W2801843945 cites W1552547581 @default.
• W2801843945 cites W1552766729 @default.
• W2801843945 cites W1553067722 @default.
• W2801843945 cites W1557451157 @default.
• W2801843945 cites W1560841381 @default.
• W2801843945 cites W1567771404 @default.
• W2801843945 cites W1568132792 @default.
• W2801843945 cites W1571748845 @default.
• W2801843945 cites W1578199049 @default.
• W2801843945 cites W1579547218 @default.
• W2801843945 cites W1579878633 @default.
• W2801843945 cites W1584590512 @default.
• W2801843945 cites W1587033109 @default.
• W2801843945 cites W1588157109 @default.
• W2801843945 cites W1588506815 @default.
• W2801843945 cites W1591068460 @default.
• W2801843945 cites W1593690756 @default.
• W2801843945 cites W1597998272 @default.
• W2801843945 cites W1604517259 @default.
• W2801843945 cites W1606739417 @default.
• W2801843945 cites W1606830172 @default.
• W2801843945 cites W1608880291 @default.
• W2801843945 cites W1613555203 @default.
• W2801843945 cites W1625194831 @default.
• W2801843945 cites W1628209714 @default.
• W2801843945 cites W1637919740 @default.
• W2801843945 cites W1644007388 @default.
• W2801843945 cites W1645614786 @default.
• W2801843945 cites W1695142101 @default.
• W2801843945 cites W1702322095 @default.
• W2801843945 cites W1715558547 @default.
• W2801843945 cites W1724931609 @default.
• W2801843945 cites W1731704781 @default.
• W2801843945 cites W1747422055 @default.
• W2801843945 cites W1755429381 @default.
• W2801843945 cites W1761097802 @default.
• W2801843945 cites W1762854803 @default.
• W2801843945 cites W1770262177 @default.
• W2801843945 cites W1780232586 @default.
• W2801843945 cites W1793190047 @default.
• W2801843945 cites W1799661214 @default.
• W2801843945 cites W1804886209 @default.
• W2801843945 cites W183116738 @default.
• W2801843945 cites W1839702653 @default.
• W2801843945 cites W1877231298 @default.
• W2801843945 cites W1877901924 @default.
• W2801843945 cites W1881510917 @default.
• W2801843945 cites W1893979131 @default.
• W2801843945 cites W1902535716 @default.
• W2801843945 cites W1912738419 @default.
• W2801843945 cites W1915290077 @default.
• W2801843945 cites W1915601792 @default.
• W2801843945 cites W1927596245 @default.
• W2801843945 cites W1929155715 @default.
• W2801843945 cites W1930160686 @default.
• W2801843945 cites W1935484321 @default.
• W2801843945 cites W1957190841 @default.
• W2801843945 cites W1957665850 @default.
• W2801843945 cites W1960003086 @default.
• W2801843945 cites W1963580819 @default.
• W2801843945 cites W1963889567 @default.
• W2801843945 cites W1964297270 @default.
• W2801843945 cites W1966795037 @default.
• W2801843945 cites W1966885880 @default.
• W2801843945 cites W1967738350 @default.
• W2801843945 cites W1973920773 @default.
• W2801843945 cites W1975790879 @default.
• W2801843945 cites W1976494728 @default.
• W2801843945 cites W1977511110 @default.
• W2801843945 cites W1979775862 @default.

• next