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- W280202846 abstract "Novel actin-like protein (NAP) is a highly divergent actin expressed in Chlamydomonas. With its low sequence similarity, it is uncertain whether NAP can polymerize into filaments. Here I assessed it by ectopically expressing enhanced green fluorescent protein-tagged NAP (EGFP-NAP) in cultured cells. EGFP-NAP was excluded from stress fibres but partially co-localized with endogenous actin in the cell periphery. In fluorescence recovery after photobleaching experiment, turnover rate of EGFP-NAP was similar to the estimated diffusion rate of monomeric actin. Therefore, EGFP-NAP likely accumulates by diffusion. These findings suggest that NAP has extremely poor ability to polymerize." @default.
- W280202846 created "2016-06-24" @default.
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- W280202846 date "1955-02-01" @default.
- W280202846 modified "2023-10-14" @default.
- W280202846 title "Engineering analysis" @default.
- W280202846 doi "https://doi.org/10.1016/0016-0032(55)90940-6" @default.
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