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- W2802040679 abstract "Cell‐penetrating peptides (CPPs) are commonly defined by their shared ability to be internalized into eukaryotic cells, without inducing permanent membrane damage, and to improve cargo delivery. Many CPPs also possess antimicrobial action strong enough to selectively lyse microbes in infected mammalian cultures. pVEC, a CPP derived from cadherin, is able to translocate into mammalian cells, and it is also antimicrobial. Structure‐activity relationship and sequence alignment studies have suggested that the hydrophobic N‐terminus (LLIIL) of pVEC is essential for this peptide's uptake into eukaryotic cells. In this study, our aim was to examine the contribution of these residues to the antimicrobial action and the translocation mechanism of pVEC. We performed antimicrobial activity and microscopy experiments with pVEC and with del5 pVEC (N‐terminal truncated variant of pVEC) and showed that pVEC loses its antimicrobial effect upon deletion of the LLIIL residues, even though both peptides induce membrane permeability. We also calculated the free energy of the transport process using steered molecular dynamic simulations and replica exchange umbrella sampling simulations to compare the difference in uptake mechanism of the 2 peptides in atomistic detail. Despite the difference in experimentally observed antimicrobial activity, the simulations on the 2 peptides showed similar characteristics and the energetic cost of translocation of pVEC was higher than that of del5 pVEC, suggesting that pVEC uptake mechanism cannot be explained by simple passive transport. Our results suggest that LLIIL residues are key contributors to pVEC antibacterial activity because of irreversible membrane disruption." @default.
- W2802040679 created "2018-05-17" @default.
- W2802040679 creator A5002264469 @default.
- W2802040679 creator A5013237196 @default.
- W2802040679 creator A5061247461 @default.
- W2802040679 date "2018-05-08" @default.
- W2802040679 modified "2023-09-27" @default.
- W2802040679 title "pVEC hydrophobic N-terminus is critical for antibacterial activity" @default.
- W2802040679 cites W1504486349 @default.
- W2802040679 cites W1964758803 @default.
- W2802040679 cites W1972729210 @default.
- W2802040679 cites W1974456685 @default.
- W2802040679 cites W1977359354 @default.
- W2802040679 cites W1979047891 @default.
- W2802040679 cites W1984877105 @default.
- W2802040679 cites W1985113094 @default.
- W2802040679 cites W1990733397 @default.
- W2802040679 cites W1990771438 @default.
- W2802040679 cites W1994076503 @default.
- W2802040679 cites W1995318118 @default.
- W2802040679 cites W1996685148 @default.
- W2802040679 cites W1996805840 @default.
- W2802040679 cites W1999061920 @default.
- W2802040679 cites W2003726865 @default.
- W2802040679 cites W2007307122 @default.
- W2802040679 cites W2008082372 @default.
- W2802040679 cites W2015481485 @default.
- W2802040679 cites W2018489122 @default.
- W2802040679 cites W2025329055 @default.
- W2802040679 cites W2029667189 @default.
- W2802040679 cites W2042143995 @default.
- W2802040679 cites W2042635806 @default.
- W2802040679 cites W2044077839 @default.
- W2802040679 cites W2055154757 @default.
- W2802040679 cites W2058919317 @default.
- W2802040679 cites W2058971275 @default.
- W2802040679 cites W2059844062 @default.
- W2802040679 cites W2060872117 @default.
- W2802040679 cites W2063191476 @default.
- W2802040679 cites W2063254598 @default.
- W2802040679 cites W2066414494 @default.
- W2802040679 cites W2066531623 @default.
- W2802040679 cites W2067174909 @default.
- W2802040679 cites W2070521942 @default.
- W2802040679 cites W2070753604 @default.
- W2802040679 cites W2073623394 @default.
- W2802040679 cites W2076515696 @default.
- W2802040679 cites W2077102218 @default.
- W2802040679 cites W2077480295 @default.
- W2802040679 cites W2079881883 @default.
- W2802040679 cites W2080645020 @default.
- W2802040679 cites W2081702863 @default.
- W2802040679 cites W2082468518 @default.
- W2802040679 cites W2082624736 @default.
- W2802040679 cites W2089573429 @default.
- W2802040679 cites W2092000721 @default.
- W2802040679 cites W2112689110 @default.
- W2802040679 cites W2130444227 @default.
- W2802040679 cites W2150981663 @default.
- W2802040679 cites W2152270594 @default.
- W2802040679 cites W2161288893 @default.
- W2802040679 cites W2162076458 @default.
- W2802040679 cites W2206954826 @default.
- W2802040679 cites W2244867214 @default.
- W2802040679 cites W2305144260 @default.
- W2802040679 cites W2320357709 @default.
- W2802040679 cites W2323602858 @default.
- W2802040679 cites W2429193927 @default.
- W2802040679 cites W2524443989 @default.
- W2802040679 cites W2527303815 @default.
- W2802040679 cites W2530145611 @default.
- W2802040679 cites W2563325144 @default.
- W2802040679 cites W2566284695 @default.
- W2802040679 cites W2567713795 @default.
- W2802040679 cites W2588262749 @default.
- W2802040679 cites W2593637926 @default.
- W2802040679 cites W2606246233 @default.
- W2802040679 cites W2610611378 @default.
- W2802040679 cites W2638251159 @default.
- W2802040679 cites W2735909856 @default.
- W2802040679 cites W2736250812 @default.
- W2802040679 cites W2736423475 @default.
- W2802040679 cites W2748518023 @default.
- W2802040679 cites W2767686567 @default.
- W2802040679 cites W2783614941 @default.
- W2802040679 cites W2783718385 @default.
- W2802040679 cites W2783819151 @default.
- W2802040679 cites W2786810290 @default.
- W2802040679 cites W2790301939 @default.
- W2802040679 cites W3100565380 @default.
- W2802040679 cites W4239028853 @default.
- W2802040679 doi "https://doi.org/10.1002/psc.3083" @default.
- W2802040679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29737576" @default.
- W2802040679 hasPublicationYear "2018" @default.
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