FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2802043971> ?p ?o ?g. }

• W2802043971 endingPage "156" @default.
• W2802043971 startingPage "152" @default.
• W2802043971 abstract "The correct identification of anti-dense fine speckled-70 (DFS70) antibodies represents an important issue in the detection of anti-nuclear antibodies (ANAs) as performed by the indirect immunofluorescence (IIF) test on HEp-2 substrates. In this study, we have evaluated a new method for anti-DFS70 antibody detection employing HEp-2 cells knocked-out for the DFS70 antigen.We studied 148 sera with a DFS70-like pattern (91 positive and 57 negative when tested for anti-DFS70 antibodies by a specific chemoluminescence [CLIA] method); 116 sera with infectious disease; 100 healthy donors (HDs), 139 samples from patients with a defined diagnosis of autoimmune rheumatic disease (ARD), and 242 consecutive unselected samples screened for ANA during the routine work-up.The HEp2 DFS70-Ko substrate recognized anti-DFS70 antibodies in 86/91 (94.5%) of the DFS70 CLIA-positive sera and in 9/57 (15.8%) of the DFS70 CLIA-negative samples. None of the 116 infectious diseases were positive for DFS70 using the engineered IIF substrate. Two samples (2%) were positive among HDs and were then confirmed by CLIA. The 139 ANA-positive sera from patients with ARD displaying a defined antibody specificity showed their expected patterns also on DFS70-Ko HEp-2 substrate. Five of the 242 (2.1%) consecutive samples tested in the routine ANA-screening were identified as DFS70-positive using the HEp2 Ko-substrate and were then confirmed by CLIA.The use of DFS70 HEp-2 Ko cells may offer the unique possibility of simultaneously identifying and confirming the presence of anti-DFS70 antibodies during the standard ANA evaluation, while keeping the expression of other autoantibody markers intact." @default.
• W2802043971 created "2018-05-17" @default.
• W2802043971 creator A5005393599 @default.
• W2802043971 creator A5044733599 @default.
• W2802043971 date "2018-05-06" @default.
• W2802043971 modified "2023-10-03" @default.
• W2802043971 title "New genetically engineered DFS70 knock-out HEp-2 cells enable rapid and specific recognition of anti-DFS70 antibodies" @default.
• W2802043971 cites W1530211033 @default.
• W2802043971 cites W1986435586 @default.
• W2802043971 cites W2006491771 @default.
• W2802043971 cites W2021973801 @default.
• W2802043971 cites W2026845136 @default.
• W2802043971 cites W2033958009 @default.
• W2802043971 cites W2034034623 @default.
• W2802043971 cites W2061851924 @default.
• W2802043971 cites W2065617048 @default.
• W2802043971 cites W2070527591 @default.
• W2802043971 cites W2075160216 @default.
• W2802043971 cites W2119583266 @default.
• W2802043971 cites W2122657747 @default.
• W2802043971 cites W2133337718 @default.
• W2802043971 cites W2159860161 @default.
• W2802043971 cites W2163888646 @default.
• W2802043971 cites W2174832170 @default.
• W2802043971 cites W2175679498 @default.
• W2802043971 cites W2188299787 @default.
• W2802043971 cites W2189066539 @default.
• W2802043971 cites W2237216692 @default.
• W2802043971 cites W2396182279 @default.
• W2802043971 cites W2411911851 @default.
• W2802043971 cites W2465168462 @default.
• W2802043971 cites W2466820081 @default.
• W2802043971 cites W2473275850 @default.
• W2802043971 cites W2498151893 @default.
• W2802043971 cites W2505022122 @default.
• W2802043971 cites W2555131970 @default.
• W2802043971 cites W2591058303 @default.
• W2802043971 cites W2597299251 @default.
• W2802043971 cites W979024514 @default.
• W2802043971 doi "https://doi.org/10.1080/08916934.2018.1469013" @default.
• W2802043971 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29732925" @default.
• W2802043971 hasPublicationYear "2018" @default.
• W2802043971 type Work @default.
• W2802043971 sameAs 2802043971 @default.
• W2802043971 citedByCount "13" @default.
• W2802043971 countsByYear W28020439712019 @default.
• W2802043971 countsByYear W28020439712020 @default.
• W2802043971 countsByYear W28020439712021 @default.
• W2802043971 countsByYear W28020439712022 @default.
• W2802043971 countsByYear W28020439712023 @default.
• W2802043971 crossrefType "journal-article" @default.
• W2802043971 hasAuthorship W2802043971A5005393599 @default.
• W2802043971 hasAuthorship W2802043971A5044733599 @default.
• W2802043971 hasConcept C101180180 @default.
• W2802043971 hasConcept C149443304 @default.
• W2802043971 hasConcept C153911025 @default.
• W2802043971 hasConcept C159654299 @default.
• W2802043971 hasConcept C163764329 @default.
• W2802043971 hasConcept C185592680 @default.
• W2802043971 hasConcept C203014093 @default.
• W2802043971 hasConcept C2778236600 @default.
• W2802043971 hasConcept C2984894117 @default.
• W2802043971 hasConcept C71924100 @default.
• W2802043971 hasConcept C86803240 @default.
• W2802043971 hasConceptScore W2802043971C101180180 @default.
• W2802043971 hasConceptScore W2802043971C149443304 @default.
• W2802043971 hasConceptScore W2802043971C153911025 @default.
• W2802043971 hasConceptScore W2802043971C159654299 @default.
• W2802043971 hasConceptScore W2802043971C163764329 @default.
• W2802043971 hasConceptScore W2802043971C185592680 @default.
• W2802043971 hasConceptScore W2802043971C203014093 @default.
• W2802043971 hasConceptScore W2802043971C2778236600 @default.
• W2802043971 hasConceptScore W2802043971C2984894117 @default.
• W2802043971 hasConceptScore W2802043971C71924100 @default.
• W2802043971 hasConceptScore W2802043971C86803240 @default.
• W2802043971 hasIssue "4" @default.
• W2802043971 hasLocation W28020439711 @default.
• W2802043971 hasLocation W28020439712 @default.
• W2802043971 hasOpenAccess W2802043971 @default.
• W2802043971 hasPrimaryLocation W28020439711 @default.
• W2802043971 hasRelatedWork W2056257362 @default.
• W2802043971 hasRelatedWork W2093747755 @default.
• W2802043971 hasRelatedWork W2167816255 @default.
• W2802043971 hasRelatedWork W2358755758 @default.
• W2802043971 hasRelatedWork W2369071334 @default.
• W2802043971 hasRelatedWork W2491040862 @default.
• W2802043971 hasRelatedWork W3092832540 @default.
• W2802043971 hasRelatedWork W4212975127 @default.
• W2802043971 hasRelatedWork W4220977287 @default.
• W2802043971 hasRelatedWork W4310463192 @default.
• W2802043971 hasVolume "51" @default.
• W2802043971 isParatext "false" @default.
• W2802043971 isRetracted "false" @default.
• W2802043971 magId "2802043971" @default.
• W2802043971 workType "article" @default.