FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2802103151> ?p ?o ?g. }

• W2802103151 abstract "High glucose combined with high FFAs can contribute to the unfavorable development of type 2 diabetes mellitus (T2DM) and monocytes/macrophages are important in the occurrence and development of T2DM, which is regarded as a type of low‑grade inflammation. Although our previous study demonstrated that increased expression of P2X7 receptor (P2X7R) in peripheral blood monocytes may alter the innate immune system and that long non‑coding (lnc)RNA uc.48+ was involved in diabetic neuropathic pain, the involvement of uc.48+ mediated by the P2X7R in monocyte/macrophages during T2DM has not been reported. In the present study, the effectsof uc.48+ small interference RNA (siRNA) on factors, including the mRNA and protein expression of P2X7R, apoptosis and proliferation, levels of reactive oxygen species (ROS), cytokine levels, and expression of phosphorylated (p‑) extracellular signal‑regulated kinase (ERK)1/2, were examined in RAW264.7 macrophages following exposure to high glucose and high plasma free fatty acids (FFAs). After RAW264.7 cells were transfected with uc.48+ siRNA under high glucose conditions and FFAs treatment, the mRNA expression levels of uc.48+ and P2X7 receptor were detected by reverse transcription‑polymerase chain reaction. The protein mass of P2X7 receptor and ERK signaling pathway were assessed by western blotting. ROS and calcium concentrations, and culture supernatant cytokine content [tumor necrosis factor‑α, interleukin (IL)‑10, IL‑1β] were detected by fluorescent probes and ELISA respectively. Cell viability and apoptosis were determined by MTS test and flow cytometry, respectively. It was found that treatment of RAW264.7 cells with high glucose and FFAs, which exhibited increased expression of uc.48+, evoked P2X7R‑mediated immune and inflammatory responses through several means, including cytokine secretion, ROS formation, and activation of the ERK signaling pathway. The uc.48+ siRNA regulated these factors and thus influenced the course and outcome of the immune and inflammatory responses mediated by P2X7R." @default.
• W2802103151 created "2018-05-17" @default.
• W2802103151 creator A5052437697 @default.
• W2802103151 creator A5055988784 @default.
• W2802103151 creator A5057055806 @default.
• W2802103151 creator A5080015131 @default.
• W2802103151 creator A5087141133 @default.
• W2802103151 date "2018-05-09" @default.
• W2802103151 modified "2023-10-07" @default.
• W2802103151 title "LncRNA uc.48+ is involved in the diabetic immune and inflammatory responses mediated by P2X7 receptor in RAW264.7 macrophages" @default.
• W2802103151 cites W1559664171 @default.
• W2802103151 cites W1701460655 @default.
• W2802103151 cites W1701760291 @default.
• W2802103151 cites W1899027652 @default.
• W2802103151 cites W1969669561 @default.
• W2802103151 cites W1969944420 @default.
• W2802103151 cites W1982121134 @default.
• W2802103151 cites W2010500550 @default.
• W2802103151 cites W2045378165 @default.
• W2802103151 cites W2055974945 @default.
• W2802103151 cites W2058821562 @default.
• W2802103151 cites W2061588426 @default.
• W2802103151 cites W2062756785 @default.
• W2802103151 cites W2083277816 @default.
• W2802103151 cites W2087268320 @default.
• W2802103151 cites W2096884191 @default.
• W2802103151 cites W2100990361 @default.
• W2802103151 cites W2102019113 @default.
• W2802103151 cites W2120809475 @default.
• W2802103151 cites W2128554001 @default.
• W2802103151 cites W2144088689 @default.
• W2802103151 cites W2145461802 @default.
• W2802103151 cites W2148395609 @default.
• W2802103151 cites W2152575235 @default.
• W2802103151 cites W2165275387 @default.
• W2802103151 cites W2165705576 @default.
• W2802103151 cites W2166130014 @default.
• W2802103151 cites W2279786413 @default.
• W2802103151 cites W2290321013 @default.
• W2802103151 cites W2326761376 @default.
• W2802103151 cites W2340554437 @default.
• W2802103151 cites W2342823001 @default.
• W2802103151 cites W2410983342 @default.
• W2802103151 cites W2417897466 @default.
• W2802103151 cites W2507352079 @default.
• W2802103151 cites W2527508290 @default.
• W2802103151 doi "https://doi.org/10.3892/ijmm.2018.3661" @default.
• W2802103151 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29750294" @default.
• W2802103151 hasPublicationYear "2018" @default.
• W2802103151 type Work @default.
• W2802103151 sameAs 2802103151 @default.
• W2802103151 citedByCount "12" @default.
• W2802103151 countsByYear W28021031512018 @default.
• W2802103151 countsByYear W28021031512019 @default.
• W2802103151 countsByYear W28021031512020 @default.
• W2802103151 countsByYear W28021031512021 @default.
• W2802103151 countsByYear W28021031512022 @default.
• W2802103151 countsByYear W28021031512023 @default.
• W2802103151 crossrefType "journal-article" @default.
• W2802103151 hasAuthorship W2802103151A5052437697 @default.
• W2802103151 hasAuthorship W2802103151A5055988784 @default.
• W2802103151 hasAuthorship W2802103151A5057055806 @default.
• W2802103151 hasAuthorship W2802103151A5080015131 @default.
• W2802103151 hasAuthorship W2802103151A5087141133 @default.
• W2802103151 hasBestOaLocation W28021031511 @default.
• W2802103151 hasConcept C153911025 @default.
• W2802103151 hasConcept C170493617 @default.
• W2802103151 hasConcept C17991360 @default.
• W2802103151 hasConcept C190283241 @default.
• W2802103151 hasConcept C203014093 @default.
• W2802103151 hasConcept C2776914184 @default.
• W2802103151 hasConcept C2778690821 @default.
• W2802103151 hasConcept C2781184567 @default.
• W2802103151 hasConcept C502942594 @default.
• W2802103151 hasConcept C553184892 @default.
• W2802103151 hasConcept C55493867 @default.
• W2802103151 hasConcept C57074206 @default.
• W2802103151 hasConcept C62478195 @default.
• W2802103151 hasConcept C86803240 @default.
• W2802103151 hasConcept C8891405 @default.
• W2802103151 hasConcept C95444343 @default.
• W2802103151 hasConceptScore W2802103151C153911025 @default.
• W2802103151 hasConceptScore W2802103151C170493617 @default.
• W2802103151 hasConceptScore W2802103151C17991360 @default.
• W2802103151 hasConceptScore W2802103151C190283241 @default.
• W2802103151 hasConceptScore W2802103151C203014093 @default.
• W2802103151 hasConceptScore W2802103151C2776914184 @default.
• W2802103151 hasConceptScore W2802103151C2778690821 @default.
• W2802103151 hasConceptScore W2802103151C2781184567 @default.
• W2802103151 hasConceptScore W2802103151C502942594 @default.
• W2802103151 hasConceptScore W2802103151C553184892 @default.
• W2802103151 hasConceptScore W2802103151C55493867 @default.
• W2802103151 hasConceptScore W2802103151C57074206 @default.
• W2802103151 hasConceptScore W2802103151C62478195 @default.
• W2802103151 hasConceptScore W2802103151C86803240 @default.
• W2802103151 hasConceptScore W2802103151C8891405 @default.
• W2802103151 hasConceptScore W2802103151C95444343 @default.
• W2802103151 hasLocation W28021031511 @default.
• W2802103151 hasLocation W28021031512 @default.
• W2802103151 hasOpenAccess W2802103151 @default.

• next