Matches in SemOpenAlex for { <https://semopenalex.org/work/W2802190243> ?p ?o ?g. }
- W2802190243 abstract "Summary During DNA replication, conflicts with ongoing transcription are frequent and require careful management to avoid genetic instability. R-loops, three stranded nucleic acid structures comprising a DNA:RNA hybrid and displaced single stranded DNA, are important drivers of damage arising from such conflicts. How R-loops stall replication and the mechanisms that restrain their formation during S phase are incompletely understood. Here we show in vivo how R-loop formation drives a short purine-rich repeat, (GAA) 10 , to become a replication impediment that requires the repriming activity of the primase-polymerase PrimPol for its processive replication. Further, we show that loss of PrimPol results in a significant increase in R-loop formation around the repeat during S phase. We extend this observation by showing that PrimPol suppresses R-loop formation in genes harbouring secondary structure-forming sequences, exemplified by G quadruplex and H-DNA motifs, across the genome in both avian and human cells. Thus, R-loops promote the creation of replication blocks at susceptible sequences, while PrimPol-dependent repriming limits the extent of unscheduled R-loop formation at these sequences, mitigating their impact on replication." @default.
- W2802190243 created "2018-05-17" @default.
- W2802190243 creator A5009302053 @default.
- W2802190243 creator A5009491330 @default.
- W2802190243 creator A5010454412 @default.
- W2802190243 creator A5024859074 @default.
- W2802190243 creator A5030663340 @default.
- W2802190243 creator A5040900596 @default.
- W2802190243 creator A5064891550 @default.
- W2802190243 creator A5070637982 @default.
- W2802190243 date "2018-05-09" @default.
- W2802190243 modified "2023-09-26" @default.
- W2802190243 title "S phase R-loop formation is restricted by PrimPol-mediated repriming" @default.
- W2802190243 cites W1481975749 @default.
- W2802190243 cites W1539559164 @default.
- W2802190243 cites W1568843108 @default.
- W2802190243 cites W1798173089 @default.
- W2802190243 cites W1967512385 @default.
- W2802190243 cites W1968488865 @default.
- W2802190243 cites W1970469121 @default.
- W2802190243 cites W1971888755 @default.
- W2802190243 cites W1976736675 @default.
- W2802190243 cites W1977709885 @default.
- W2802190243 cites W1984582197 @default.
- W2802190243 cites W1989540449 @default.
- W2802190243 cites W1991616035 @default.
- W2802190243 cites W1996435746 @default.
- W2802190243 cites W2001865748 @default.
- W2802190243 cites W2019643706 @default.
- W2802190243 cites W2024374899 @default.
- W2802190243 cites W2024800350 @default.
- W2802190243 cites W2031816743 @default.
- W2802190243 cites W2033619661 @default.
- W2802190243 cites W2038185005 @default.
- W2802190243 cites W2041538415 @default.
- W2802190243 cites W2041626076 @default.
- W2802190243 cites W2043375872 @default.
- W2802190243 cites W2044375974 @default.
- W2802190243 cites W2046147149 @default.
- W2802190243 cites W2051058497 @default.
- W2802190243 cites W2051234541 @default.
- W2802190243 cites W2054681032 @default.
- W2802190243 cites W2057648499 @default.
- W2802190243 cites W2058940094 @default.
- W2802190243 cites W2062810119 @default.
- W2802190243 cites W2064809185 @default.
- W2802190243 cites W2067613039 @default.
- W2802190243 cites W2071652837 @default.
- W2802190243 cites W2072710826 @default.
- W2802190243 cites W2076790103 @default.
- W2802190243 cites W2082223307 @default.
- W2802190243 cites W2082286833 @default.
- W2802190243 cites W2086307439 @default.
- W2802190243 cites W2088784554 @default.
- W2802190243 cites W2090081292 @default.
- W2802190243 cites W2093146349 @default.
- W2802190243 cites W2100440853 @default.
- W2802190243 cites W2102619694 @default.
- W2802190243 cites W2117099883 @default.
- W2802190243 cites W2123017921 @default.
- W2802190243 cites W2124561138 @default.
- W2802190243 cites W2124764875 @default.
- W2802190243 cites W2129515289 @default.
- W2802190243 cites W2134573093 @default.
- W2802190243 cites W2137770909 @default.
- W2802190243 cites W2138903411 @default.
- W2802190243 cites W2139864835 @default.
- W2802190243 cites W2153526603 @default.
- W2802190243 cites W2156174123 @default.
- W2802190243 cites W2156435713 @default.
- W2802190243 cites W2158155007 @default.
- W2802190243 cites W2160336344 @default.
- W2802190243 cites W2163445749 @default.
- W2802190243 cites W2166887457 @default.
- W2802190243 cites W2169745877 @default.
- W2802190243 cites W2170551349 @default.
- W2802190243 cites W2178614563 @default.
- W2802190243 cites W2255556690 @default.
- W2802190243 cites W23639935 @default.
- W2802190243 cites W2394692813 @default.
- W2802190243 cites W2408638299 @default.
- W2802190243 cites W2468705417 @default.
- W2802190243 cites W2516400840 @default.
- W2802190243 cites W2516481335 @default.
- W2802190243 cites W2539844013 @default.
- W2802190243 cites W2542820697 @default.
- W2802190243 cites W2556186518 @default.
- W2802190243 cites W2590835604 @default.
- W2802190243 cites W2600482014 @default.
- W2802190243 cites W2619370444 @default.
- W2802190243 cites W2730080362 @default.
- W2802190243 cites W2743954317 @default.
- W2802190243 cites W2771049305 @default.
- W2802190243 cites W2787975097 @default.
- W2802190243 doi "https://doi.org/10.1101/318220" @default.
- W2802190243 hasPublicationYear "2018" @default.
- W2802190243 type Work @default.
- W2802190243 sameAs 2802190243 @default.
- W2802190243 citedByCount "3" @default.
- W2802190243 countsByYear W28021902432019 @default.