Matches in SemOpenAlex for { <https://semopenalex.org/work/W2802264022> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2802264022 abstract "To use the tandem mass tags (TMT) based quantitative proteomics technique and bioinformatics method to identify potential serum diagnostic biomarkers for gastric cancer (GC).This study enrolled GC patients and healthy control subjects. Mixed serum samples were pooled with 10 individual samples. The high-abundance proteins depleted serum proteins were collected by removing abundance albumin and immunoglobulin G (IgG). After desalting and ultrafiltration, the trypsin digested proteins were analyzed using TMT based quantitative proteomics system. The differential proteins were screened using the cutoff value of 1.2-fold change for up-regulation or down-regulation. The gene ontology (GO) was further analyzed using the UniProtKB/Swiss-Prot database. Then the differentially expressed protein ITIH4, S100A8, CD59, and COF1 were conducted using western blot.A total of 594 distinct serum proteins were identified between the GC group and the healthy controls. Forty-eight proteins were up-regulated and 57 were down-regulated using the cutoff value of 1.2-fold change. Using bioinformatics analysis, we found that the differentially expressed proteins were mainly concentrated in the extracellular exosome, extracellular region, extracellular space, and plasma membrane; their biological process activities included antigen binding, calcium ion binding, and protein homodimerization. In addition, the western blotting results showed that four differentially expressed proteins were completely coincident with the TMT quantification trend.The results showed that we were able to successfully create the differentially expressed protein database of GC using TMT technology. These proteins are potential molecular markers that could help us understand the potential molecular mechanism of GC." @default.
- W2802264022 created "2018-05-17" @default.
- W2802264022 creator A5034109008 @default.
- W2802264022 creator A5034321500 @default.
- W2802264022 creator A5039167830 @default.
- W2802264022 creator A5048507557 @default.
- W2802264022 date "2018-01-01" @default.
- W2802264022 modified "2023-09-25" @default.
- W2802264022 title "Serum Proteomic Analysis by Tandem Mass Tags (TMT) Based Quantitative Proteomics in Gastric Cancer Patients" @default.
- W2802264022 doi "https://doi.org/10.7754/clin.lab.2018.171129" @default.
- W2802264022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29739064" @default.
- W2802264022 hasPublicationYear "2018" @default.
- W2802264022 type Work @default.
- W2802264022 sameAs 2802264022 @default.
- W2802264022 citedByCount "15" @default.
- W2802264022 countsByYear W28022640222020 @default.
- W2802264022 countsByYear W28022640222021 @default.
- W2802264022 countsByYear W28022640222022 @default.
- W2802264022 countsByYear W28022640222023 @default.
- W2802264022 crossrefType "journal-article" @default.
- W2802264022 hasAuthorship W2802264022A5034109008 @default.
- W2802264022 hasAuthorship W2802264022A5034321500 @default.
- W2802264022 hasAuthorship W2802264022A5039167830 @default.
- W2802264022 hasAuthorship W2802264022A5048507557 @default.
- W2802264022 hasConcept C104317684 @default.
- W2802264022 hasConcept C104397665 @default.
- W2802264022 hasConcept C146543888 @default.
- W2802264022 hasConcept C150194340 @default.
- W2802264022 hasConcept C153911025 @default.
- W2802264022 hasConcept C156534840 @default.
- W2802264022 hasConcept C185592680 @default.
- W2802264022 hasConcept C2776415932 @default.
- W2802264022 hasConcept C2779018089 @default.
- W2802264022 hasConcept C46111723 @default.
- W2802264022 hasConcept C55493867 @default.
- W2802264022 hasConcept C80311884 @default.
- W2802264022 hasConcept C85265743 @default.
- W2802264022 hasConcept C86153913 @default.
- W2802264022 hasConcept C86803240 @default.
- W2802264022 hasConceptScore W2802264022C104317684 @default.
- W2802264022 hasConceptScore W2802264022C104397665 @default.
- W2802264022 hasConceptScore W2802264022C146543888 @default.
- W2802264022 hasConceptScore W2802264022C150194340 @default.
- W2802264022 hasConceptScore W2802264022C153911025 @default.
- W2802264022 hasConceptScore W2802264022C156534840 @default.
- W2802264022 hasConceptScore W2802264022C185592680 @default.
- W2802264022 hasConceptScore W2802264022C2776415932 @default.
- W2802264022 hasConceptScore W2802264022C2779018089 @default.
- W2802264022 hasConceptScore W2802264022C46111723 @default.
- W2802264022 hasConceptScore W2802264022C55493867 @default.
- W2802264022 hasConceptScore W2802264022C80311884 @default.
- W2802264022 hasConceptScore W2802264022C85265743 @default.
- W2802264022 hasConceptScore W2802264022C86153913 @default.
- W2802264022 hasConceptScore W2802264022C86803240 @default.
- W2802264022 hasIssue "07+08/2018" @default.
- W2802264022 hasLocation W28022640221 @default.
- W2802264022 hasLocation W28022640222 @default.
- W2802264022 hasOpenAccess W2802264022 @default.
- W2802264022 hasPrimaryLocation W28022640221 @default.
- W2802264022 hasRelatedWork W2405991977 @default.
- W2802264022 hasRelatedWork W2409848145 @default.
- W2802264022 hasRelatedWork W2436516455 @default.
- W2802264022 hasRelatedWork W3024933276 @default.
- W2802264022 hasRelatedWork W3198635680 @default.
- W2802264022 hasRelatedWork W4220774682 @default.
- W2802264022 hasRelatedWork W4307747561 @default.
- W2802264022 hasRelatedWork W4310337201 @default.
- W2802264022 hasRelatedWork W4366254344 @default.
- W2802264022 hasRelatedWork W4385575857 @default.
- W2802264022 hasVolume "64" @default.
- W2802264022 isParatext "false" @default.
- W2802264022 isRetracted "false" @default.
- W2802264022 magId "2802264022" @default.
- W2802264022 workType "article" @default.