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• W2802294356 abstract "Isothermal nucleic acid amplification methods can potentially shorten the amount of time required to diagnose influenza. We developed and evaluated a novel isothermal nucleic acid amplification method, RT-SIBA to rapidly detect and differentiate between influenza A and B viruses in a single reaction tube. The performance of the RT-SIBA Influenza assay was compared with two established RT-PCR methods. The sensitivities of the RT-SIBA, RealStar RT-PCR, and CDC RT-PCR assays for the detection of influenza A and B viruses in the clinical specimens were 98.8%, 100%, and 89.3%, respectively. All three assays demonstrated a specificity of 100%. The average time to positive result was significantly shorter with the RT-SIBA Influenza assay (<20 min) than with the two RT-PCR assays (>90 min). The method can be run using battery-operated, portable devices with a small footprint and therefore has potential applications in both laboratory and near-patient settings." @default.
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• W2802294356 date "2018-09-01" @default.
• W2802294356 modified "2023-10-10" @default.
• W2802294356 title "Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens" @default.
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• W2802294356 doi "https://doi.org/10.1016/j.diagmicrobio.2018.04.006" @default.
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