FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2802410967> ?p ?o ?g. }

• W2802410967 endingPage "2049" @default.
• W2802410967 startingPage "2049" @default.
• W2802410967 abstract "HomePlant DiseaseVol. 102, No. 10First Report of Carrot torrado virus 1 and Carrot thin leaf virus Naturally Infecting Torilis arvensis ssp. arvensis in Greece PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Carrot torrado virus 1 and Carrot thin leaf virus Naturally Infecting Torilis arvensis ssp. arvensis in GreeceL. Lotos, A. Olmos, N. I. Katis, and V. I. MaliogkaL. LotosSearch for more papers by this author, A. OlmosSearch for more papers by this author, N. I. KatisSearch for more papers by this author, and V. I. Maliogka†Corresponding author: V. I. Maliogka; E-mail: E-mail Address: vmaliogk@agro.auth.grhttp://orcid.org/0000-0001-5714-2710Search for more papers by this authorAffiliationsAuthors and Affiliations L. Lotos , Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece A. Olmos , IVIA, Plant Protection and Biotechnology, 46113 Moncada, Valencia, Spain N. I. Katis V. I. Maliogka † , Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Published Online:31 Jul 2018https://doi.org/10.1094/PDIS-03-18-0381-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Plants of the Apiaceae family are susceptible to many viruses. These viral infections cause yield reduction and leaf symptoms that affect product marketability. In Greece little is known about the number, spread, and importance of viruses infecting apiaceous plant species. During a survey investigating the presence of poleroviruses in different hosts, a wild Torilis arvensis ssp. arvensis plant (sample RL5) exhibiting leaf reddening symptoms was subjected to high-throughput sequencing of small RNAs, extracted with the mirPremier microRNA Isolation Kit (Sigma-Aldrich, St. Louis, MO) from leaves and stems, on an Ion Torrent platform. The run yielded 1,809,132 reads in the 21 to 24 nt range. De novo assembly of these reads and subsequent blast analysis (BLASTn/x) of the 2,512 contigs that were produced revealed, apart from a polerovirus-like sequence (data not shown), the presence of two additional viruses, Carrot thin leaf virus (CTLV, genus Potyvirus, family Potyviridae) and Carrot torrado virus 1 (CaTV1, genus Torradovirus, family Secoviridae). Blasting revealed that 129 de novo contigs from 40 to 255 nt in length were similar to CTLV, and 138 from 40 to 410 nt to CaTV1. The complete genome of CTLV (isolate RL5-Pot, accession no. LT615233) was reconstructed using iterative mapping of the 21- to 24-nt reads to the de novo contigs, with 233,894 reads mapped in total. Similarly, 38,545 reads produced the complete RNA2 of CaTV1 (isolate RL5-Tor, accession no. LT615234), and 9,508 reads the ≈85% of RNA1. Similarity analysis with the isolates deposited in the online databases (CTLV, JX156434 and JX156435; CaTV1, KF533719 and KF533720) showed 85.9% identities in nucleotides for CTLV-RL5-Pot and 77.3 and 94.7% identities in nucleotides for RNA1 (of the 85% that was reconstructed) and RNA2 of CaTV1-RL5-Tor, respectively. To detect these viruses in sample RL5, two sets of primers were designed, Tor3185-Up (5′-CTTTCCACCAATATTATGCAAGTTCC-3′) and Tor3854-Down (5′-ATAAAAAATCCACTCCGCTTCTTCC-3′) and Pot8068-Up (5′-GAAGTTCGGATGCTAGATGAGTTAC-3′) and Pot8820-Down (5′-GTTGAATTGGTTGTGTGTTGATCTTG-3′), which amplify 670 and 753 nt of CaTV1-RNA2 and CTLV, respectively. Reverse transcription polymerase chain reaction assays produced the expected amplicons for both viruses in sample RL5, and Sanger sequencing of the amplicons confirmed the sequence obtained by next-generation sequencing. Thirty-two additional samples from different cultivated and wild apiaceous plant species were screened for both viruses, and only one was found to be infected with CaTV1. The infected plant was a wild carrot (Daucus carota) (sample UmNp1) originating from a different location, ≈200 km away from the collection site of RL5. CaTV1 was originally identified by Adams et al. (2014) in the United Kingdom and in 2017 it was reported in France (Rozado-Aguirre et al. 2017). The virus has been previously detected in cultivated carrots; however, this is a first report from naturally infected wild hosts, which could potentially act as its reservoirs in the field. CTLV has been known for many years; however, its full sequence was only recently obtained by Xu et al. (2014). That study also revealed that CTLV can infect a number of different wild and cultivated species, but so far it has not been reported in T. arvensis. This is the first report of these two viruses in Greece, thus extending the information on their geographical distribution. Additional screenings are necessary to evaluate the presence and impact of CaTV1 and CTLV in Apiaceae crops in Greece.References:Adams, I., et al. 2014. PLoS One 9:e109125. https://doi.org/10.1371/journal.pone.0109125 Crossref, ISI, Google ScholarRozado-Aguirre, Z., et al. 2017. Plant Dis. 101:1333. https://doi.org/10.1094/PDIS-01-17-0095-PDN Link, ISI, Google ScholarXu, D., et al. 2014. Eur. J. Plant Pathol. 138:15. https://doi.org/10.1007/s10658-013-0310-8 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 102, No. 10 October 2018SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 28 Sep 2018Published: 31 Jul 2018First Look: 17 Apr 2018Accepted: 16 Apr 2018 Pages: 2049-2049 Information© 2018 The American Phytopathological SocietyCited byEnhanced Apiaceous Potyvirus Phylogeny, Novel Viruses, and New Country and Host Records from Sequencing Apiaceae Samples27 July 2022 | Plants, Vol. 11, No. 15Carrot thin leaf virusCABI Compendium, Vol. CABI CompendiumCarrot torradovirus 1CABI Compendium, Vol. CABI CompendiumPest categorisation of carrot thin leaf virusEFSA Journal, Vol. 19, No. 12Complete Genome Sequence of a Carrot Torradovirus 1 Isolate, Obtained from Angelica keiskei in JapanMicrobiology Resource Announcements, Vol. 8, No. 15" @default.
• W2802410967 created "2018-05-17" @default.
• W2802410967 creator A5003548065 @default.
• W2802410967 creator A5027407873 @default.
• W2802410967 creator A5049916682 @default.
• W2802410967 creator A5083895143 @default.
• W2802410967 date "2018-10-01" @default.
• W2802410967 modified "2023-10-14" @default.
• W2802410967 title "First Report of <i>Carrot torrado virus 1</i> and <i>Carrot thin leaf virus</i> Naturally Infecting <i>Torilis arvensis</i> ssp. <i>arvensis</i> in Greece" @default.
• W2802410967 cites W2021366526 @default.
• W2802410967 cites W2065198508 @default.
• W2802410967 cites W2600902612 @default.
• W2802410967 doi "https://doi.org/10.1094/pdis-03-18-0381-pdn" @default.
• W2802410967 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30064341" @default.
• W2802410967 hasPublicationYear "2018" @default.
• W2802410967 type Work @default.
• W2802410967 sameAs 2802410967 @default.
• W2802410967 citedByCount "7" @default.
• W2802410967 countsByYear W28024109672019 @default.
• W2802410967 countsByYear W28024109672021 @default.
• W2802410967 countsByYear W28024109672022 @default.
• W2802410967 countsByYear W28024109672023 @default.
• W2802410967 crossrefType "journal-article" @default.
• W2802410967 hasAuthorship W2802410967A5003548065 @default.
• W2802410967 hasAuthorship W2802410967A5027407873 @default.
• W2802410967 hasAuthorship W2802410967A5049916682 @default.
• W2802410967 hasAuthorship W2802410967A5083895143 @default.
• W2802410967 hasBestOaLocation W28024109671 @default.
• W2802410967 hasConcept C109110057 @default.
• W2802410967 hasConcept C144027150 @default.
• W2802410967 hasConcept C159047783 @default.
• W2802410967 hasConcept C2522874641 @default.
• W2802410967 hasConcept C2777278459 @default.
• W2802410967 hasConcept C2778587282 @default.
• W2802410967 hasConcept C59822182 @default.
• W2802410967 hasConcept C86803240 @default.
• W2802410967 hasConceptScore W2802410967C109110057 @default.
• W2802410967 hasConceptScore W2802410967C144027150 @default.
• W2802410967 hasConceptScore W2802410967C159047783 @default.
• W2802410967 hasConceptScore W2802410967C2522874641 @default.
• W2802410967 hasConceptScore W2802410967C2777278459 @default.
• W2802410967 hasConceptScore W2802410967C2778587282 @default.
• W2802410967 hasConceptScore W2802410967C59822182 @default.
• W2802410967 hasConceptScore W2802410967C86803240 @default.
• W2802410967 hasIssue "10" @default.
• W2802410967 hasLocation W28024109671 @default.
• W2802410967 hasLocation W28024109672 @default.
• W2802410967 hasOpenAccess W2802410967 @default.
• W2802410967 hasPrimaryLocation W28024109671 @default.
• W2802410967 hasRelatedWork W2023271816 @default.
• W2802410967 hasRelatedWork W2027989916 @default.
• W2802410967 hasRelatedWork W2047134548 @default.
• W2802410967 hasRelatedWork W2085710752 @default.
• W2802410967 hasRelatedWork W2119976708 @default.
• W2802410967 hasRelatedWork W2981713487 @default.
• W2802410967 hasRelatedWork W3042563890 @default.
• W2802410967 hasRelatedWork W3215139134 @default.
• W2802410967 hasRelatedWork W4220788934 @default.
• W2802410967 hasRelatedWork W770724554 @default.
• W2802410967 hasVolume "102" @default.
• W2802410967 isParatext "false" @default.
• W2802410967 isRetracted "false" @default.
• W2802410967 magId "2802410967" @default.
• W2802410967 workType "article" @default.