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- W2802610588 endingPage "e12860" @default.
- W2802610588 startingPage "e12860" @default.
- W2802610588 abstract "To study the mechanisms of placenta function and the role of extracellular vesicles (EVs) in pregnancy, it is necessary to develop an ex vivo system that retains placental cytoarchitecture and the primary metabolic aspects, in particular the release of EVs and soluble factors. Here, we developed such a system and investigated the pattern of secretion of cytokines, growth factors, and extracellular vesicles by placental villous and amnion tissues ex vivo.Placental villous and amnion explants were cultured for 2 weeks at the air/liquid interface and their morphology and the released cytokines and EVs were analyzed. Cytokines were analyzed with multiplexed bead assays, and individual EVs were analyzed with recently developed techniques that involved EV capture with magnetic nanoparticles coupled to anti-EV antibodies and flow cytometry.Ex vivo tissues (i) remained viable and preserved their cytoarchitecture; (ii) maintained secretion of cytokines and growth factors; (iii) released EVs of syncytiotrophoblast and amnion epithelial cell origins that contain cytokines and growth factors.A system of ex vivo placental villous and amnion tissues can be used as an adequate model to study placenta metabolic activity in normal and complicated pregnancies, in particular to characterize EVs by their surface markers and by encapsulated proteins. Establishment and benchmarking the placenta ex vivo system may provide new insight in the functional status of this organ in various placental disorders, particularly regarding the release of EVs and cytokines. Such EVs may have a prognostic value for pregnancy complications." @default.
- W2802610588 created "2018-05-17" @default.
- W2802610588 creator A5012191640 @default.
- W2802610588 creator A5017279874 @default.
- W2802610588 creator A5053982479 @default.
- W2802610588 creator A5067511554 @default.
- W2802610588 creator A5085524384 @default.
- W2802610588 date "2018-05-04" @default.
- W2802610588 modified "2023-10-18" @default.
- W2802610588 title "Extracellular vesicles generated by placental tissues ex vivo: A transport system for immune mediators and growth factors" @default.
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