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• W2802685608 abstract "Human aldo-keto reductase 1C3 (AKR1C3) stereospecifically reduces steroids and prostaglandins and is involved in the biotransformation of xenobiotics. Its role in various cancers makes it a potential therapeutic target for the development of inhibitors. Recombinant AKR1C3 with a thrombin-cleavable N-terminal His 6 tag was expressed from a pET-28(+) vector for structural studies of enzyme–inhibitor complexes. A modified in situ proteolysis approach was applied to specifically remove the His tag by thrombin cleavage during crystallization screening trials. This improved the morphology and diffraction quality of the crystals and allowed the acquisition of high-resolution diffraction data and structure solution. This approach may be generally applicable to other proteins expressed using the pET-28(+) vector." @default.
• W2802685608 created "2018-05-17" @default.
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• W2802685608 date "2018-04-24" @default.
• W2802685608 modified "2023-10-18" @default.
• W2802685608 title "<i>In situ</i>proteolysis of an N-terminal His tag with thrombin improves the diffraction quality of human aldo-keto reductase 1C3 crystals" @default.
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• W2802685608 doi "https://doi.org/10.1107/s2053230x18005721" @default.
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