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• W2802816462 abstract "High-throughput RNA-sequencing (RNA-seq) technologies provide an unprecedented opportunity to explore the individual transcriptome. Unmapped reads are a large and often overlooked output of standard RNA-seq analyses. Here, we present Read Origin Protocol (ROP), a tool for discovering the source of all reads originating from complex RNA molecules. We apply ROP to samples across 2630 individuals from 54 diverse human tissues. Our approach can account for 99.9% of 1 trillion reads of various read length. Additionally, we use ROP to investigate the functional mechanisms underlying connections between the immune system, microbiome, and disease. ROP is freely available at https://github.com/smangul1/rop/wiki ." @default.
• W2802816462 created "2018-05-17" @default.
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• W2802816462 date "2018-02-15" @default.
• W2802816462 modified "2023-10-17" @default.
• W2802816462 title "ROP: dumpster diving in RNA-sequencing to find the source of 1 trillion reads across diverse adult human tissues" @default.
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• W2802816462 doi "https://doi.org/10.1186/s13059-018-1403-7" @default.
• W2802816462 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5857127" @default.
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