Matches in SemOpenAlex for { <https://semopenalex.org/work/W2802897788> ?p ?o ?g. }
- W2802897788 endingPage "1060" @default.
- W2802897788 startingPage "1043" @default.
- W2802897788 abstract "The modulation of expression levels of fluorescent fusion proteins ( FFP s) is central for recombinant DNA technologies in modern biology as overexpression of proteins contributes to artifacts in biological experiments. In addition, some microscopy techniques such as fluorescence correlation spectroscopy ( FCS ) and single‐molecule‐based techniques are very sensitive to high expression levels of FFP s. To reduce the levels of recombinant protein expression in comparison with the commonly used, very strong CMV promoter, the herpes simplex virus thymidine kinase ( TK ) gene promoter, and mutants thereof were analyzed. Deletion mutants of the TK promoter were constructed and introduced into the Gateway ® system for ectopic expression of enhanced green fluorescent protein ( eGFP ), monomeric cherry ( mC herry), and FFP s containing these FP s. Two promoter constructs, TK 2 ST and TKTSC , were established, which have optimal low expression levels suitable for FCS studies in U2 OS , HeLa CCL 2, NIH 3T3, and BALB /c cells. Interestingly, when tested in these four cell lines, promoter constructs having a deletion within TK gene 5′‐ UTR showed significantly higher protein expression levels than the equivalent constructs lacking this deletion. This suggests that a negative regulatory element is localized within the TK gene 5′‐UTR." @default.
- W2802897788 created "2018-05-17" @default.
- W2802897788 creator A5030012610 @default.
- W2802897788 creator A5036890979 @default.
- W2802897788 creator A5089877192 @default.
- W2802897788 creator A5090427264 @default.
- W2802897788 date "2018-05-08" @default.
- W2802897788 modified "2023-10-05" @default.
- W2802897788 title "Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences" @default.
- W2802897788 cites W1572613752 @default.
- W2802897788 cites W1606142774 @default.
- W2802897788 cites W1642882929 @default.
- W2802897788 cites W1772903849 @default.
- W2802897788 cites W1785757387 @default.
- W2802897788 cites W1832820770 @default.
- W2802897788 cites W1838326446 @default.
- W2802897788 cites W1967348837 @default.
- W2802897788 cites W1970327721 @default.
- W2802897788 cites W1974874497 @default.
- W2802897788 cites W1980635292 @default.
- W2802897788 cites W1981765336 @default.
- W2802897788 cites W1985283191 @default.
- W2802897788 cites W2000137108 @default.
- W2802897788 cites W2001522957 @default.
- W2802897788 cites W2001971083 @default.
- W2802897788 cites W2005539293 @default.
- W2802897788 cites W2007315524 @default.
- W2802897788 cites W2011899109 @default.
- W2802897788 cites W2024526152 @default.
- W2802897788 cites W2027429271 @default.
- W2802897788 cites W2031258370 @default.
- W2802897788 cites W2031562047 @default.
- W2802897788 cites W2037652466 @default.
- W2802897788 cites W2039746015 @default.
- W2802897788 cites W2042577649 @default.
- W2802897788 cites W2059223045 @default.
- W2802897788 cites W2062299199 @default.
- W2802897788 cites W2063710108 @default.
- W2802897788 cites W2066278365 @default.
- W2802897788 cites W2069208087 @default.
- W2802897788 cites W2076997608 @default.
- W2802897788 cites W2080562735 @default.
- W2802897788 cites W2089843624 @default.
- W2802897788 cites W2095745022 @default.
- W2802897788 cites W2095963288 @default.
- W2802897788 cites W2098571862 @default.
- W2802897788 cites W2098781753 @default.
- W2802897788 cites W2099518138 @default.
- W2802897788 cites W2108620620 @default.
- W2802897788 cites W2108914600 @default.
- W2802897788 cites W2115191599 @default.
- W2802897788 cites W2115734610 @default.
- W2802897788 cites W2119774673 @default.
- W2802897788 cites W2123859219 @default.
- W2802897788 cites W2129258490 @default.
- W2802897788 cites W2135318926 @default.
- W2802897788 cites W2140824698 @default.
- W2802897788 cites W2143094150 @default.
- W2802897788 cites W2144337984 @default.
- W2802897788 cites W2145668963 @default.
- W2802897788 cites W2147372875 @default.
- W2802897788 cites W2152052780 @default.
- W2802897788 cites W2153335351 @default.
- W2802897788 cites W2154241726 @default.
- W2802897788 cites W2157589212 @default.
- W2802897788 cites W2161707155 @default.
- W2802897788 cites W2163106706 @default.
- W2802897788 cites W2166966739 @default.
- W2802897788 cites W2167258646 @default.
- W2802897788 cites W2170127333 @default.
- W2802897788 cites W2193995799 @default.
- W2802897788 cites W2209793704 @default.
- W2802897788 cites W2234324103 @default.
- W2802897788 cites W2277842883 @default.
- W2802897788 cites W2278584106 @default.
- W2802897788 cites W2288329098 @default.
- W2802897788 cites W2324632559 @default.
- W2802897788 cites W2325814231 @default.
- W2802897788 cites W2462894283 @default.
- W2802897788 cites W2521039464 @default.
- W2802897788 cites W2547764448 @default.
- W2802897788 cites W2584598422 @default.
- W2802897788 cites W2781885983 @default.
- W2802897788 cites W2544370958 @default.
- W2802897788 doi "https://doi.org/10.1002/2211-5463.12432" @default.
- W2802897788 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5985997" @default.
- W2802897788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29928582" @default.
- W2802897788 hasPublicationYear "2018" @default.
- W2802897788 type Work @default.
- W2802897788 sameAs 2802897788 @default.
- W2802897788 citedByCount "13" @default.
- W2802897788 countsByYear W28028977882019 @default.
- W2802897788 countsByYear W28028977882020 @default.
- W2802897788 countsByYear W28028977882021 @default.
- W2802897788 countsByYear W28028977882022 @default.
- W2802897788 countsByYear W28028977882023 @default.
- W2802897788 crossrefType "journal-article" @default.
- W2802897788 hasAuthorship W2802897788A5030012610 @default.