Matches in SemOpenAlex for { <https://semopenalex.org/work/W2802919075> ?p ?o ?g. }
- W2802919075 endingPage "122" @default.
- W2802919075 startingPage "118" @default.
- W2802919075 abstract "Many wild and domestic donkey breeds are now at risk of extinction, therefore there is an urgent need for genetic conservation of these breeds. The development of in vitro conditions that support oocyte maturation, fertilization, and embryo development may improve in vitro production of donkey embryos. This work was designed to identify the optimal condition for in vitro production of donkey embryos. Two experiments were conducted. In experiment 1, grade 1 and 2 cumulus-oocyte complexes were subjected to in vitro maturation in the following media: tissue culture medium 199 (TCM199), Christopher Rozenkrans 1aa medium (CR1aa), Christopher Rozenkrans 2aa medium (CR2aa), potassium simplex optimized medium (KSOMaa), centrifuged follicular fluid (CFF), or non-CFF from preovulatory follicles. In experiment 2, in vitro–matured donkey oocytes were subjected to in vitro fertilization (IVF) in Fert-TALP medium supplemented with 0%, 25%, 50%, 75%, or 100% CFF. After fertilization, the cleavage rates and embryo development were assessed across groups. The results of experiment 1 revealed that non-CFF and KSOMaa significantly increase cumulus cell expansion. The percentage of oocytes reaching the metaphase II stage nuclear maturation was significantly higher for donkey oocytes matured in non-CFF. Also, the percentage of degenerated oocytes was lower for donkey oocytes matured in vitro in non-CFF compared with those in TCM199, CR1aa, CR2aa, KSOMaa, or CFF. In experiment 2, IVF of in vitro–matured donkey oocytes using 100% CFF as IVF medium produced a significantly higher fertilization rate with respect to the number of fertilized oocytes extruding the second polar body. In conclusion, non-CFF from preovulatory follicles gave the highest cumulus cell expansion and nuclear maturation rates for in vitro–matured donkey oocytes. Hundred percent CFF was the best medium for IVF of matured donkey oocytes; however, cleavage rate and embryo development are still very low and need further investigations." @default.
- W2802919075 created "2018-05-17" @default.
- W2802919075 creator A5002292587 @default.
- W2802919075 creator A5010162674 @default.
- W2802919075 creator A5049832520 @default.
- W2802919075 creator A5052078954 @default.
- W2802919075 creator A5064972717 @default.
- W2802919075 creator A5074226117 @default.
- W2802919075 date "2018-06-01" @default.
- W2802919075 modified "2023-10-13" @default.
- W2802919075 title "In Vitro Maturation and Fertilization of Donkey Oocytes" @default.
- W2802919075 cites W1498035697 @default.
- W2802919075 cites W1765571020 @default.
- W2802919075 cites W195913618 @default.
- W2802919075 cites W1971083923 @default.
- W2802919075 cites W1980865387 @default.
- W2802919075 cites W2006067725 @default.
- W2802919075 cites W2016316748 @default.
- W2802919075 cites W2030771022 @default.
- W2802919075 cites W2043945675 @default.
- W2802919075 cites W2044075193 @default.
- W2802919075 cites W2053010552 @default.
- W2802919075 cites W2053493160 @default.
- W2802919075 cites W2055840724 @default.
- W2802919075 cites W2064745146 @default.
- W2802919075 cites W2068133977 @default.
- W2802919075 cites W2072309819 @default.
- W2802919075 cites W2088392966 @default.
- W2802919075 cites W2093474869 @default.
- W2802919075 cites W2097622930 @default.
- W2802919075 cites W2107371769 @default.
- W2802919075 cites W2108245059 @default.
- W2802919075 cites W2110930557 @default.
- W2802919075 cites W2122691272 @default.
- W2802919075 cites W2158119378 @default.
- W2802919075 cites W2164657462 @default.
- W2802919075 cites W2169956871 @default.
- W2802919075 cites W2172043522 @default.
- W2802919075 cites W2754063364 @default.
- W2802919075 doi "https://doi.org/10.1016/j.jevs.2018.04.007" @default.
- W2802919075 hasPublicationYear "2018" @default.
- W2802919075 type Work @default.
- W2802919075 sameAs 2802919075 @default.
- W2802919075 citedByCount "4" @default.
- W2802919075 countsByYear W28029190752019 @default.
- W2802919075 countsByYear W28029190752021 @default.
- W2802919075 countsByYear W28029190752022 @default.
- W2802919075 countsByYear W28029190752023 @default.
- W2802919075 crossrefType "journal-article" @default.
- W2802919075 hasAuthorship W2802919075A5002292587 @default.
- W2802919075 hasAuthorship W2802919075A5010162674 @default.
- W2802919075 hasAuthorship W2802919075A5049832520 @default.
- W2802919075 hasAuthorship W2802919075A5052078954 @default.
- W2802919075 hasAuthorship W2802919075A5064972717 @default.
- W2802919075 hasAuthorship W2802919075A5074226117 @default.
- W2802919075 hasConcept C105702510 @default.
- W2802919075 hasConcept C16685009 @default.
- W2802919075 hasConcept C187785154 @default.
- W2802919075 hasConcept C18903297 @default.
- W2802919075 hasConcept C196843134 @default.
- W2802919075 hasConcept C2775966511 @default.
- W2802919075 hasConcept C2776690073 @default.
- W2802919075 hasConcept C2779192065 @default.
- W2802919075 hasConcept C2779335785 @default.
- W2802919075 hasConcept C512716672 @default.
- W2802919075 hasConcept C54355233 @default.
- W2802919075 hasConcept C71924100 @default.
- W2802919075 hasConcept C86803240 @default.
- W2802919075 hasConcept C87073359 @default.
- W2802919075 hasConcept C88972607 @default.
- W2802919075 hasConceptScore W2802919075C105702510 @default.
- W2802919075 hasConceptScore W2802919075C16685009 @default.
- W2802919075 hasConceptScore W2802919075C187785154 @default.
- W2802919075 hasConceptScore W2802919075C18903297 @default.
- W2802919075 hasConceptScore W2802919075C196843134 @default.
- W2802919075 hasConceptScore W2802919075C2775966511 @default.
- W2802919075 hasConceptScore W2802919075C2776690073 @default.
- W2802919075 hasConceptScore W2802919075C2779192065 @default.
- W2802919075 hasConceptScore W2802919075C2779335785 @default.
- W2802919075 hasConceptScore W2802919075C512716672 @default.
- W2802919075 hasConceptScore W2802919075C54355233 @default.
- W2802919075 hasConceptScore W2802919075C71924100 @default.
- W2802919075 hasConceptScore W2802919075C86803240 @default.
- W2802919075 hasConceptScore W2802919075C87073359 @default.
- W2802919075 hasConceptScore W2802919075C88972607 @default.
- W2802919075 hasLocation W28029190751 @default.
- W2802919075 hasOpenAccess W2802919075 @default.
- W2802919075 hasPrimaryLocation W28029190751 @default.
- W2802919075 hasRelatedWork W2030062533 @default.
- W2802919075 hasRelatedWork W2365967871 @default.
- W2802919075 hasRelatedWork W2379507142 @default.
- W2802919075 hasRelatedWork W2380153654 @default.
- W2802919075 hasRelatedWork W2384897150 @default.
- W2802919075 hasRelatedWork W2594555206 @default.
- W2802919075 hasRelatedWork W2884789220 @default.
- W2802919075 hasRelatedWork W3134793556 @default.
- W2802919075 hasRelatedWork W3188781221 @default.
- W2802919075 hasRelatedWork W4312598131 @default.