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- W2802992018 abstract "BACKGROUND/OBJECTIVES Respiratory dysfunction and/or failure from acute lung injury (ALI) are common in acute pancreatitis (AP), but assessment of ALI in experimental AP has lacked standardisation. METHODS A range of experimental AP models induced in C57BL/6 mice with corresponding controls (n = 6/group). Full double lung or right lung specimens were taken for histopathological assessment and slides analysed by a pre-set pipeline using Aperio Scanner (Leica), ImageJ software and CellProfiler software. Findings were compared to other routinely assessed parameters. RESULTS Overall histopathological changes were similar between both lungs. Mean lung field occupancy was significantly different between moderate and severe CER-AP (21.9% v 27.5%, p < 0.05) and corresponded with lung MPO and local injury severity parameters and was mirrored for all models tested. CONCLUSION We have developed a novel, simple method for assessment of ALI to improve measurement of systemic organ injury in experimental AP and contribute to preclinical drug development." @default.
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- W2802992018 date "2018-07-01" @default.
- W2802992018 modified "2023-09-27" @default.
- W2802992018 title "A novel digital method to assess air space loss associated with acute lung injury in experimental acute pancreatitis" @default.
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- W2802992018 doi "https://doi.org/10.1016/j.pan.2018.04.017" @default.
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