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• W2803042275 abstract "Malaria caused by Plasmodium vivax is a neglected disease which is responsible for the highest morbidity in both Americas and Asia. Despite continuous public health efforts to prevent malarial infection, an effective antimalarial vaccine is still urgently needed. P. vivax vaccine development involves analyzing naturally-infected patients’ immune response to the specific proteins involved in red blood cell invasion. The P. vivax rhoptry neck protein 2 (PvRON2) is a highly conserved protein which is expressed in late schizont rhoptries; it interacts directly with AMA-1 and might be involved in moving-junction formation. Bioinformatics approaches were used here to select B- and T-cell epitopes. Eleven high-affinity binding peptides were selected using the NetMHCIIpan-3.0 in silico prediction tool; their in vitro binding to HLA-DRB1*0401, HLA-DRB1*0701, HLA-DRB1*1101 or HLA-DRB1*1302 was experimentally assessed. Four peptides (39152 (HLA-DRB1*04 and 11), 39047 (HLA-DRB1*07), 39154 (HLADRB1*13) and universal peptide 39153) evoked a naturally-acquired T-cell immune response in P. vivax-exposed individuals from two endemic areas in Colombia. All four peptides had an SI greater than 2 in proliferation assays; however, only peptides 39154 and 39153 had significant differences compared to the control group. Peptide 39047 was able to significantly stimulate TNF and IL-10 production while 39154 stimulated TNF production. Allele-specific peptides (but not the universal one) were able to stimulate IL-6 production; however, none induced IFN-γ production. The Bepipred 1.0 tool was used for selecting four B-cell epitopes in silico regarding humoral response. Peptide 39041 was the only one recognized by P. vivax-exposed individuals’ sera and had significant differences concerning IgG subclasses; an IgG2 > IgG4 profile was observed for this peptide, agreeing with a protection-inducing role against P. falciparum and P. vivax as previously described for antigens such as RESA and MSP2. The bioinformatics results and in vitro evaluation reported here highlighted two T-cell peptides (39047 and 39154) being recognized by memory cells and a B-cell epitope (39041) identified by P. vivax-exposed individuals’ sera which could be used as potential candidates when designing a subunit-based vaccine." @default.
• W2803042275 created "2018-05-17" @default.
• W2803042275 creator A5012421959 @default.
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• W2803042275 creator A5042652382 @default.
• W2803042275 creator A5070679510 @default.
• W2803042275 creator A5074613601 @default.
• W2803042275 date "2018-05-15" @default.
• W2803042275 modified "2023-09-25" @default.
• W2803042275 title "The in Vitro Antigenicity of Plasmodium vivax Rhoptry Neck Protein 2 (PvRON2) B- and T-Epitopes Selected by HLA-DRB1 Binding Profile" @default.
• W2803042275 cites W1553821274 @default.
• W2803042275 cites W1898558133 @default.
• W2803042275 cites W1964706690 @default.
• W2803042275 cites W1968378375 @default.
• W2803042275 cites W1975388516 @default.
• W2803042275 cites W1978484898 @default.
• W2803042275 cites W1986450327 @default.
• W2803042275 cites W1989513077 @default.
• W2803042275 cites W1994616010 @default.
• W2803042275 cites W1994761559 @default.
• W2803042275 cites W1994847450 @default.
• W2803042275 cites W2000486917 @default.
• W2803042275 cites W2001573980 @default.
• W2803042275 cites W2004545272 @default.
• W2803042275 cites W2005592215 @default.
• W2803042275 cites W2017310475 @default.
• W2803042275 cites W2017321063 @default.
• W2803042275 cites W2018234499 @default.
• W2803042275 cites W2021002908 @default.
• W2803042275 cites W2024183493 @default.
• W2803042275 cites W2026483075 @default.
• W2803042275 cites W2029075055 @default.
• W2803042275 cites W2031342255 @default.
• W2803042275 cites W2033970156 @default.
• W2803042275 cites W2034306306 @default.
• W2803042275 cites W2042758260 @default.
• W2803042275 cites W2050658429 @default.
• W2803042275 cites W2052944434 @default.
• W2803042275 cites W2056140371 @default.
• W2803042275 cites W2061111624 @default.
• W2803042275 cites W2069331462 @default.
• W2803042275 cites W2069918510 @default.
• W2803042275 cites W2075817888 @default.
• W2803042275 cites W2080018424 @default.
• W2803042275 cites W2080956225 @default.
• W2803042275 cites W2083547256 @default.
• W2803042275 cites W2084180944 @default.
• W2803042275 cites W2087944044 @default.
• W2803042275 cites W2089035318 @default.
• W2803042275 cites W2091238395 @default.
• W2803042275 cites W2094215362 @default.
• W2803042275 cites W2097432479 @default.
• W2803042275 cites W2099295104 @default.
• W2803042275 cites W2102237050 @default.
• W2803042275 cites W2107435367 @default.
• W2803042275 cites W2112047656 @default.
• W2803042275 cites W2117680953 @default.
• W2803042275 cites W2118060089 @default.
• W2803042275 cites W2118632050 @default.
• W2803042275 cites W2119130553 @default.
• W2803042275 cites W2120399005 @default.
• W2803042275 cites W2122779464 @default.
• W2803042275 cites W2123797310 @default.
• W2803042275 cites W2127284149 @default.
• W2803042275 cites W2129088316 @default.
• W2803042275 cites W2133400749 @default.
• W2803042275 cites W2133465839 @default.
• W2803042275 cites W2138956025 @default.
• W2803042275 cites W2139424510 @default.
• W2803042275 cites W2140148815 @default.
• W2803042275 cites W2140878929 @default.
• W2803042275 cites W2144841307 @default.
• W2803042275 cites W2149350146 @default.
• W2803042275 cites W2155966745 @default.
• W2803042275 cites W2156436289 @default.
• W2803042275 cites W2157038428 @default.
• W2803042275 cites W2160397907 @default.
• W2803042275 cites W2167965520 @default.
• W2803042275 cites W2274234437 @default.
• W2803042275 cites W2467614481 @default.
• W2803042275 cites W2544105246 @default.
• W2803042275 cites W2555449609 @default.
• W2803042275 cites W2586086082 @default.
• W2803042275 cites W2589029503 @default.
• W2803042275 cites W2590085129 @default.
• W2803042275 cites W2614379161 @default.
• W2803042275 cites W2615422713 @default.
• W2803042275 cites W2618648656 @default.
• W2803042275 cites W2747600376 @default.
• W2803042275 cites W2748069344 @default.
• W2803042275 cites W2748378282 @default.
• W2803042275 cites W2749318676 @default.
• W2803042275 cites W2760279547 @default.
• W2803042275 cites W415123709 @default.
• W2803042275 doi "https://doi.org/10.3389/fcimb.2018.00156" @default.
• W2803042275 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5962679" @default.
• W2803042275 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29868512" @default.
• W2803042275 hasPublicationYear "2018" @default.
• W2803042275 type Work @default.
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