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- W2803114686 abstract "ABSTRACT Targeted RNA-sequencing aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-sequencing experiments. Here we present a novel approach for targeted RNA-sequencing that uses complex pools of reverse transcription primers to enable sequencing enrichment at user-selected locations across the genome. We demonstrate this approach by targeting hundreds to thousands of pre-mRNA splice junctions, revealing high-precision detection of splice isoforms, including rare pre-mRNA splicing intermediates." @default.
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- W2803114686 date "2018-05-25" @default.
- W2803114686 modified "2023-10-05" @default.
- W2803114686 title "High Precision Detection of Rare Splice Isoforms Using Multiplexed Primer Extension Sequencing" @default.
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- W2803114686 doi "https://doi.org/10.1101/331629" @default.
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