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- W2803220171 abstract "potential applications in human health and nutrition. The molecular basis of SDA biosynthesis in thisplant remains unknown due to lack of genomic information. To unravel key genes involved in SDA-richtriacylglycerol (TAG) biosynthesis, we performed transcriptome sequencing of pooled mRNA fromfive different developmental stages of B. arvensis seeds using Illumina NextSeq platform. De novotranscriptome assembly generated 102,888 clustered transcripts from 39.83 million high-quality reads.Of these, 62.1% and 55.54% of transcripts were functionally annotated using Uniprot-Viridiplantaeand KOG databases, respectively. A total of 10,021 SSR-containing sequences were identified usingthe MISA tool. Deep mining of transcriptome assembly using in silico tools led to the identification ofgenes involved in fatty acid and TAG biosynthesis. Expression profiling of 17 key transcripts involved infatty acid desaturation and TAG biosynthesis showed expression patterns specific to the developmentstage that positively correlated with polyunsaturated fatty acid accumulation in the developing seeds.This first comprehensive transcriptome analysis provides the basis for future research on understandingmolecular mechanisms of SDA-rich TAG accumulation in B. arvensis and aids in biotechnologicalproduction of SDA in other oilseed crops." @default.
- W2803220171 created "2018-06-01" @default.
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- W2803220171 date "2017-01-01" @default.
- W2803220171 modified "2023-09-24" @default.
- W2803220171 title "Unravelling a stearidonic acidrichtriacylglycerol biosyntheticpathway in the developingseeds of Buglossoides arvensis: Atranscriptomic landscape." @default.
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