FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2803260765> ?p ?o ?g. }

• W2803260765 abstract "Abstract Background Most two‐ dimensional in vitro models for studying host‐ H. pylori interactions rely on tumor‐derived cell lines, which harbor malignant alterations. The recent development of human gastric organoids has overcome this limitation and provides a highly sophisticated, yet costly, short‐term model for H. pylori infection, with restricted use in low‐budget centers. Method Tissue specimens from upper, middle, and lower stomachs of H. pylori ‐negative volunteers were collectively dispersed and cultured on mouse embryonic fibroblast ( MEF ) or collagen‐coated plates. Gastric primary cells ( GPC s) were evaluated by light microscopy, immunostaining, qRT ‐ PCR and ELISA analysis of cellular secretions, before and after H. pylori infection. Results The formation and long‐term (up to 1 year) maintenance of GPC s was highly dependent on adherent inactivated MEF cells, cultured in enriched media. These cells were multipassageable and able to undergo stable freezer storage and subsequent revival. The cellular composition of GPC s included the combination of cytokeratin 18 ( CK 18) and E‐cadherin (E‐cad)‐positive epithelial cells, MUC 5 AC ‐positive gastric cells, and leucine‐rich repeat containing G protein‐coupled receptor 5 ( LGR 5)‐positive progenitor cells. These cells produced significant amounts of gastric pepsinogens I and II . GPC s also allowed for extended (up to 96 hours) H. pylori infection, during which they underwent morphological alterations (cellular vacuolation and elongation) and hyperproduction of gastric pepsinogens and inflammatory cytokines ( IL ‐8 and TNF ‐α). Conclusion We, hereby, present a simple, consistent, and cost‐efficient gastric cell culture system, which provides a suitable model for extended in vitro infection of H. pylori . This platform can be employed for a variety of gastric‐related research." @default.
• W2803260765 created "2018-06-01" @default.
• W2803260765 creator A5000705981 @default.
• W2803260765 creator A5026681545 @default.
• W2803260765 creator A5030581476 @default.
• W2803260765 creator A5058054110 @default.
• W2803260765 creator A5061652743 @default.
• W2803260765 creator A5065292871 @default.
• W2803260765 creator A5077735640 @default.
• W2803260765 date "2018-05-17" @default.
• W2803260765 modified "2023-10-14" @default.
• W2803260765 title "A simple and cost-efficient adherent culture platform for human gastric primary cells, as an in vitro model for<i>Helicobacter pylori</i>infection" @default.
• W2803260765 cites W1144270415 @default.
• W2803260765 cites W1504552211 @default.
• W2803260765 cites W1520868501 @default.
• W2803260765 cites W1565780948 @default.
• W2803260765 cites W1604700810 @default.
• W2803260765 cites W1722374088 @default.
• W2803260765 cites W1769758742 @default.
• W2803260765 cites W1821070749 @default.
• W2803260765 cites W1965157206 @default.
• W2803260765 cites W1968437096 @default.
• W2803260765 cites W1983304854 @default.
• W2803260765 cites W1984335288 @default.
• W2803260765 cites W1994799573 @default.
• W2803260765 cites W1996955003 @default.
• W2803260765 cites W2000940862 @default.
• W2803260765 cites W2002305721 @default.
• W2803260765 cites W2005258803 @default.
• W2803260765 cites W2006932396 @default.
• W2803260765 cites W2011177881 @default.
• W2803260765 cites W2013642124 @default.
• W2803260765 cites W2023026872 @default.
• W2803260765 cites W2031190177 @default.
• W2803260765 cites W2039155608 @default.
• W2803260765 cites W2048967371 @default.
• W2803260765 cites W2050707891 @default.
• W2803260765 cites W2052583856 @default.
• W2803260765 cites W2059993226 @default.
• W2803260765 cites W2072390114 @default.
• W2803260765 cites W2073637472 @default.
• W2803260765 cites W2085844606 @default.
• W2803260765 cites W2089840779 @default.
• W2803260765 cites W2092071393 @default.
• W2803260765 cites W2097387056 @default.
• W2803260765 cites W2098751022 @default.
• W2803260765 cites W2099621223 @default.
• W2803260765 cites W2099974330 @default.
• W2803260765 cites W2107277218 @default.
• W2803260765 cites W2107650725 @default.
• W2803260765 cites W2116168356 @default.
• W2803260765 cites W2123630792 @default.
• W2803260765 cites W2128837962 @default.
• W2803260765 cites W2131208459 @default.
• W2803260765 cites W2134281919 @default.
• W2803260765 cites W2138442302 @default.
• W2803260765 cites W2152729684 @default.
• W2803260765 cites W2157670214 @default.
• W2803260765 cites W2161538753 @default.
• W2803260765 cites W2163858677 @default.
• W2803260765 cites W2167230045 @default.
• W2803260765 cites W2168102484 @default.
• W2803260765 cites W22697574 @default.
• W2803260765 cites W2283967385 @default.
• W2803260765 cites W2332361988 @default.
• W2803260765 cites W2419168572 @default.
• W2803260765 cites W243335379 @default.
• W2803260765 cites W2516338155 @default.
• W2803260765 cites W2562023464 @default.
• W2803260765 cites W2584985211 @default.
• W2803260765 cites W313599211 @default.
• W2803260765 cites W67666564 @default.
• W2803260765 doi "https://doi.org/10.1111/hel.12489" @default.
• W2803260765 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29774633" @default.
• W2803260765 hasPublicationYear "2018" @default.
• W2803260765 type Work @default.
• W2803260765 sameAs 2803260765 @default.
• W2803260765 citedByCount "3" @default.
• W2803260765 countsByYear W28032607652019 @default.
• W2803260765 countsByYear W28032607652022 @default.
• W2803260765 crossrefType "journal-article" @default.
• W2803260765 hasAuthorship W2803260765A5000705981 @default.
• W2803260765 hasAuthorship W2803260765A5026681545 @default.
• W2803260765 hasAuthorship W2803260765A5030581476 @default.
• W2803260765 hasAuthorship W2803260765A5058054110 @default.
• W2803260765 hasAuthorship W2803260765A5061652743 @default.
• W2803260765 hasAuthorship W2803260765A5065292871 @default.
• W2803260765 hasAuthorship W2803260765A5077735640 @default.
• W2803260765 hasConcept C104317684 @default.
• W2803260765 hasConcept C142724271 @default.
• W2803260765 hasConcept C145103041 @default.
• W2803260765 hasConcept C201750760 @default.
• W2803260765 hasConcept C202751555 @default.
• W2803260765 hasConcept C203014093 @default.
• W2803260765 hasConcept C204232928 @default.
• W2803260765 hasConcept C2776409635 @default.
• W2803260765 hasConcept C2779012181 @default.
• W2803260765 hasConcept C28328180 @default.
• W2803260765 hasConcept C4224716 @default.
• W2803260765 hasConcept C54355233 @default.

• next