Matches in SemOpenAlex for { <https://semopenalex.org/work/W2803285013> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2803285013 endingPage "653" @default.
- W2803285013 startingPage "641" @default.
- W2803285013 abstract "Cholangiocarcinoma (CCA) is a malignant tumor that is resistant to chemotherapy, so new therapeutic agents are needed. Allicin which is rapidly converted from allin by allinase, is one of the most biologically active compounds in freshly crushed garlic and has been shown to have strong anti-tumor effects. Our aim was to explore the molecular mechanism by which allicin affects the cell proliferation and invasion of CCA.Cell viability and apoptosis were measured using the CCK-8 assay, colony formation assay, and flow cytometry. Cell migration and invasion were evaluated by wound healing and Transwell assays, respectively. The expression of several proteins involved in cell apoptosis and invasion were assessed by Western blot. The activation of STAT3 signaling was detected by Western blot and immunofluorescence staining. The involvement of SHP-1 was determined using small interfering RNA (siRNA). Moreover, a nude mouse model of human CCA was established to assess the anti-tumor effects of allicin in vivo.Allicin significantly suppressed CCA cell proliferation by activating the caspase cascade, inducing apoptosis, and reducing the expression of proteins downstream of STAT3, such as B-cell lymphoma 2 (Bcl-2), while upregulating Bcl-2-associated X (Bax) protein. In addition, allicin inhibited the migration, invasion, and epithelial-mesenchymal transition (EMT) of CCA cells. Moreover, the protein expression of MMP-2 and MMP-9 was significantly downregulated in CCA cells treated with allicin compared with CCA cells treated with control. Mechanistic investigations indicated that allicin upregulated SHP-1 expression in CCA, and pervanadate treatment reversed the allicin-induced downregulation of STAT3. Moreover, suppression of SHP-1 by siRNA overturned the effect of allicin on the induction of SHP-1 and inhibition of STAT3 activation. Additionally, treatment with allicin attenuated tumor growth in the nude mouse model of CCA.Our findings suggest that allicin suppresses cell proliferation and invasion via STAT3 signaling and may be a potential therapeutic agent for CCA." @default.
- W2803285013 created "2018-06-01" @default.
- W2803285013 creator A5011297387 @default.
- W2803285013 creator A5017684588 @default.
- W2803285013 creator A5023933073 @default.
- W2803285013 creator A5036278731 @default.
- W2803285013 creator A5037337693 @default.
- W2803285013 creator A5051327567 @default.
- W2803285013 creator A5059125345 @default.
- W2803285013 creator A5060282020 @default.
- W2803285013 creator A5077537812 @default.
- W2803285013 creator A5083579026 @default.
- W2803285013 date "2018-01-01" @default.
- W2803285013 modified "2023-10-07" @default.
- W2803285013 title "Allicin Inhibits Proliferation and Invasion in Vitro and in Vivo via SHP-1-Mediated STAT3 Signaling in Cholangiocarcinoma" @default.
- W2803285013 doi "https://doi.org/10.1159/000490019" @default.
- W2803285013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29794468" @default.
- W2803285013 hasPublicationYear "2018" @default.
- W2803285013 type Work @default.
- W2803285013 sameAs 2803285013 @default.
- W2803285013 citedByCount "42" @default.
- W2803285013 countsByYear W28032850132018 @default.
- W2803285013 countsByYear W28032850132019 @default.
- W2803285013 countsByYear W28032850132020 @default.
- W2803285013 countsByYear W28032850132021 @default.
- W2803285013 countsByYear W28032850132022 @default.
- W2803285013 countsByYear W28032850132023 @default.
- W2803285013 crossrefType "journal-article" @default.
- W2803285013 hasAuthorship W2803285013A5011297387 @default.
- W2803285013 hasAuthorship W2803285013A5017684588 @default.
- W2803285013 hasAuthorship W2803285013A5023933073 @default.
- W2803285013 hasAuthorship W2803285013A5036278731 @default.
- W2803285013 hasAuthorship W2803285013A5037337693 @default.
- W2803285013 hasAuthorship W2803285013A5051327567 @default.
- W2803285013 hasAuthorship W2803285013A5059125345 @default.
- W2803285013 hasAuthorship W2803285013A5060282020 @default.
- W2803285013 hasAuthorship W2803285013A5077537812 @default.
- W2803285013 hasAuthorship W2803285013A5083579026 @default.
- W2803285013 hasBestOaLocation W28032850131 @default.
- W2803285013 hasConcept C104317684 @default.
- W2803285013 hasConcept C127561419 @default.
- W2803285013 hasConcept C1491633281 @default.
- W2803285013 hasConcept C153911025 @default.
- W2803285013 hasConcept C185592680 @default.
- W2803285013 hasConcept C190283241 @default.
- W2803285013 hasConcept C22615655 @default.
- W2803285013 hasConcept C2776415932 @default.
- W2803285013 hasConcept C2781005338 @default.
- W2803285013 hasConcept C502942594 @default.
- W2803285013 hasConcept C53227056 @default.
- W2803285013 hasConcept C54009773 @default.
- W2803285013 hasConcept C54355233 @default.
- W2803285013 hasConcept C553184892 @default.
- W2803285013 hasConcept C55493867 @default.
- W2803285013 hasConcept C62112901 @default.
- W2803285013 hasConcept C81885089 @default.
- W2803285013 hasConcept C86803240 @default.
- W2803285013 hasConceptScore W2803285013C104317684 @default.
- W2803285013 hasConceptScore W2803285013C127561419 @default.
- W2803285013 hasConceptScore W2803285013C1491633281 @default.
- W2803285013 hasConceptScore W2803285013C153911025 @default.
- W2803285013 hasConceptScore W2803285013C185592680 @default.
- W2803285013 hasConceptScore W2803285013C190283241 @default.
- W2803285013 hasConceptScore W2803285013C22615655 @default.
- W2803285013 hasConceptScore W2803285013C2776415932 @default.
- W2803285013 hasConceptScore W2803285013C2781005338 @default.
- W2803285013 hasConceptScore W2803285013C502942594 @default.
- W2803285013 hasConceptScore W2803285013C53227056 @default.
- W2803285013 hasConceptScore W2803285013C54009773 @default.
- W2803285013 hasConceptScore W2803285013C54355233 @default.
- W2803285013 hasConceptScore W2803285013C553184892 @default.
- W2803285013 hasConceptScore W2803285013C55493867 @default.
- W2803285013 hasConceptScore W2803285013C62112901 @default.
- W2803285013 hasConceptScore W2803285013C81885089 @default.
- W2803285013 hasConceptScore W2803285013C86803240 @default.
- W2803285013 hasIssue "2" @default.
- W2803285013 hasLocation W28032850131 @default.
- W2803285013 hasLocation W28032850132 @default.
- W2803285013 hasLocation W28032850133 @default.
- W2803285013 hasOpenAccess W2803285013 @default.
- W2803285013 hasPrimaryLocation W28032850131 @default.
- W2803285013 hasRelatedWork W2014161583 @default.
- W2803285013 hasRelatedWork W2089385972 @default.
- W2803285013 hasRelatedWork W2145287198 @default.
- W2803285013 hasRelatedWork W2267574209 @default.
- W2803285013 hasRelatedWork W2366534284 @default.
- W2803285013 hasRelatedWork W2807312381 @default.
- W2803285013 hasRelatedWork W2891007313 @default.
- W2803285013 hasRelatedWork W2968185054 @default.
- W2803285013 hasRelatedWork W3006825483 @default.
- W2803285013 hasRelatedWork W4385971377 @default.
- W2803285013 hasVolume "47" @default.
- W2803285013 isParatext "false" @default.
- W2803285013 isRetracted "false" @default.
- W2803285013 magId "2803285013" @default.
- W2803285013 workType "article" @default.