Matches in SemOpenAlex for { <https://semopenalex.org/work/W2803660964> ?p ?o ?g. }
- W2803660964 endingPage "348" @default.
- W2803660964 startingPage "337" @default.
- W2803660964 abstract "A Gram’s positive, rod shaped and endospores-forming ‘bacterial isolate KLP15′ was identified as Bacillus subtilis (Accession number KT459335) by the biochemical tests and 16S rRNA based sequencing. Lipopeptide(s) production from B. subtilis KLP2015 increased from 200.0 to 547.0 mg/l after the optimization of physico-chemical parameters like choice of nitrogen source, fermentation time, fermentation temperature and broth pH by ‘One Factor at a Time’ (OFAT) approach. Further, the yield of lipopeptide(s) increased to 985 mg/l from an earlier value of 547 mg/l when a “Two Factors at a Time (TFAT) approach using Response Surface Methodology (RSM) was attempted. The extracted crude lipopeptide fraction from the cell-free fermentation broth of B. subtilis KLP2015 was purified and characterized by High Performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), UV-Visible Spectrophotometry (UV-vis), Fourier Transform Infrared (FTIR) and MALDI-TOF studies. HPLC and TLC results confirmed the purity of the lipopeptide preparation and its Surfactin-type nature. The FTIR and MALDI-TOF spectra of the purified lipopeptide were found to be similar to that of an authentic Surfactin molecule. The purified lipopeptide showed potent 75.1% and 41.9% antifungal activity against Mucor sp. and Aspergillus niger, respectively on the basis of zone of inhibition of growth of these fungal strains. Moreover, the purified lipopeptide preparation of B. subtilis KLP2015 was most effective against the growth of Mucor sp. as reflected by the recorded MIC values of 6.25 µg/ml while MIC of lipopeptide for the A. niger was 12.5 µg/ml." @default.
- W2803660964 created "2018-06-01" @default.
- W2803660964 creator A5004921644 @default.
- W2803660964 creator A5004956512 @default.
- W2803660964 creator A5006538669 @default.
- W2803660964 creator A5010345231 @default.
- W2803660964 date "2020-01-01" @default.
- W2803660964 modified "2023-10-17" @default.
- W2803660964 title "Two factor at a time approach by response surface methodology to aggrandize the Bacillus subtilis KLP2015 surfactin lipopeptide to use as antifungal agent" @default.
- W2803660964 cites W1964389084 @default.
- W2803660964 cites W1964834601 @default.
- W2803660964 cites W1967187304 @default.
- W2803660964 cites W1968992587 @default.
- W2803660964 cites W1978283531 @default.
- W2803660964 cites W1983974226 @default.
- W2803660964 cites W1988710527 @default.
- W2803660964 cites W2003117592 @default.
- W2803660964 cites W2010666335 @default.
- W2803660964 cites W2015420153 @default.
- W2803660964 cites W2019227237 @default.
- W2803660964 cites W2021210228 @default.
- W2803660964 cites W2025955985 @default.
- W2803660964 cites W2040234689 @default.
- W2803660964 cites W2063518823 @default.
- W2803660964 cites W2065313948 @default.
- W2803660964 cites W2071486947 @default.
- W2803660964 cites W2079675770 @default.
- W2803660964 cites W2092237875 @default.
- W2803660964 cites W2119565384 @default.
- W2803660964 cites W2125121305 @default.
- W2803660964 cites W2133556020 @default.
- W2803660964 cites W2166501045 @default.
- W2803660964 cites W2168999294 @default.
- W2803660964 cites W2170638721 @default.
- W2803660964 cites W2395808170 @default.
- W2803660964 cites W2493214797 @default.
- W2803660964 cites W2521905877 @default.
- W2803660964 cites W2603622370 @default.
- W2803660964 cites W2616623394 @default.
- W2803660964 cites W2766675044 @default.
- W2803660964 cites W327972826 @default.
- W2803660964 cites W4233060568 @default.
- W2803660964 doi "https://doi.org/10.1016/j.jksus.2018.05.025" @default.
- W2803660964 hasPublicationYear "2020" @default.
- W2803660964 type Work @default.
- W2803660964 sameAs 2803660964 @default.
- W2803660964 citedByCount "31" @default.
- W2803660964 countsByYear W28036609642019 @default.
- W2803660964 countsByYear W28036609642020 @default.
- W2803660964 countsByYear W28036609642021 @default.
- W2803660964 countsByYear W28036609642022 @default.
- W2803660964 countsByYear W28036609642023 @default.
- W2803660964 crossrefType "journal-article" @default.
- W2803660964 hasAuthorship W2803660964A5004921644 @default.
- W2803660964 hasAuthorship W2803660964A5004956512 @default.
- W2803660964 hasAuthorship W2803660964A5006538669 @default.
- W2803660964 hasAuthorship W2803660964A5010345231 @default.
- W2803660964 hasBestOaLocation W28036609641 @default.
- W2803660964 hasConcept C100544194 @default.
- W2803660964 hasConcept C179998833 @default.
- W2803660964 hasConcept C185592680 @default.
- W2803660964 hasConcept C2776102837 @default.
- W2803660964 hasConcept C2777272437 @default.
- W2803660964 hasConcept C2777425210 @default.
- W2803660964 hasConcept C43617362 @default.
- W2803660964 hasConcept C523546767 @default.
- W2803660964 hasConcept C54355233 @default.
- W2803660964 hasConcept C55493867 @default.
- W2803660964 hasConcept C86803240 @default.
- W2803660964 hasConcept C89423630 @default.
- W2803660964 hasConceptScore W2803660964C100544194 @default.
- W2803660964 hasConceptScore W2803660964C179998833 @default.
- W2803660964 hasConceptScore W2803660964C185592680 @default.
- W2803660964 hasConceptScore W2803660964C2776102837 @default.
- W2803660964 hasConceptScore W2803660964C2777272437 @default.
- W2803660964 hasConceptScore W2803660964C2777425210 @default.
- W2803660964 hasConceptScore W2803660964C43617362 @default.
- W2803660964 hasConceptScore W2803660964C523546767 @default.
- W2803660964 hasConceptScore W2803660964C54355233 @default.
- W2803660964 hasConceptScore W2803660964C55493867 @default.
- W2803660964 hasConceptScore W2803660964C86803240 @default.
- W2803660964 hasConceptScore W2803660964C89423630 @default.
- W2803660964 hasIssue "1" @default.
- W2803660964 hasLocation W28036609641 @default.
- W2803660964 hasOpenAccess W2803660964 @default.
- W2803660964 hasPrimaryLocation W28036609641 @default.
- W2803660964 hasRelatedWork W1975627845 @default.
- W2803660964 hasRelatedWork W2019203966 @default.
- W2803660964 hasRelatedWork W2029769934 @default.
- W2803660964 hasRelatedWork W2072682511 @default.
- W2803660964 hasRelatedWork W2301188081 @default.
- W2803660964 hasRelatedWork W2331932260 @default.
- W2803660964 hasRelatedWork W2387150264 @default.
- W2803660964 hasRelatedWork W3035223634 @default.
- W2803660964 hasRelatedWork W3202374504 @default.
- W2803660964 hasRelatedWork W49067265 @default.
- W2803660964 hasVolume "32" @default.
- W2803660964 isParatext "false" @default.