Matches in SemOpenAlex for { <https://semopenalex.org/work/W2803682131> ?p ?o ?g. }
- W2803682131 endingPage "93" @default.
- W2803682131 startingPage "82" @default.
- W2803682131 abstract "Methylglyoxal is a highly toxic metabolite that can be produced in all living organisms. Methylglyoxal was artificially elevated by removal of the tpiA gene from a growth optimized Escherichia coli strain. The initial response to elevated methylglyoxal and its toxicity was characterized, and detoxification mechanisms were studied using adaptive laboratory evolution. We found that: 1) Multi-omics analysis revealed biological consequences of methylglyoxal toxicity, which included attack on macromolecules including DNA and RNA and perturbation of nucleotide levels; 2) Counter-intuitive cross-talk between carbon starvation and inorganic phosphate signalling was revealed in the tpiA deletion strain that required mutations in inorganic phosphate signalling mechanisms to alleviate; and 3) The split flux through lower glycolysis depleted glycolytic intermediates requiring a host of synchronized and coordinated mutations in non-intuitive network locations in order to re-adjust the metabolic flux map to achieve optimal growth. Such mutations included a systematic inactivation of the Phosphotransferase System (PTS) and alterations in cell wall biosynthesis enzyme activity. This study demonstrated that deletion of major metabolic genes followed by ALE was a productive approach to gain novel insight into the systems biology underlying optimal phenotypic states." @default.
- W2803682131 created "2018-06-01" @default.
- W2803682131 creator A5002596498 @default.
- W2803682131 creator A5007010641 @default.
- W2803682131 creator A5009834334 @default.
- W2803682131 creator A5048081015 @default.
- W2803682131 creator A5051097609 @default.
- W2803682131 creator A5062042072 @default.
- W2803682131 creator A5071564078 @default.
- W2803682131 creator A5072991489 @default.
- W2803682131 date "2018-07-01" @default.
- W2803682131 modified "2023-10-11" @default.
- W2803682131 title "Adaptation to the coupling of glycolysis to toxic methylglyoxal production in tpiA deletion strains of Escherichia coli requires synchronized and counterintuitive genetic changes" @default.
- W2803682131 cites W1504582786 @default.
- W2803682131 cites W1646462907 @default.
- W2803682131 cites W1757241200 @default.
- W2803682131 cites W1838917369 @default.
- W2803682131 cites W1852859757 @default.
- W2803682131 cites W1923409750 @default.
- W2803682131 cites W1964649339 @default.
- W2803682131 cites W1966234811 @default.
- W2803682131 cites W1966669851 @default.
- W2803682131 cites W1967494222 @default.
- W2803682131 cites W1969887535 @default.
- W2803682131 cites W1971023242 @default.
- W2803682131 cites W1978860492 @default.
- W2803682131 cites W1985347638 @default.
- W2803682131 cites W1990125045 @default.
- W2803682131 cites W1990705181 @default.
- W2803682131 cites W1991194699 @default.
- W2803682131 cites W1997604729 @default.
- W2803682131 cites W1997718636 @default.
- W2803682131 cites W1998673116 @default.
- W2803682131 cites W2002085843 @default.
- W2803682131 cites W2016350681 @default.
- W2803682131 cites W2024032813 @default.
- W2803682131 cites W2026382071 @default.
- W2803682131 cites W2028340483 @default.
- W2803682131 cites W2031859095 @default.
- W2803682131 cites W2032453778 @default.
- W2803682131 cites W2033280568 @default.
- W2803682131 cites W2033557504 @default.
- W2803682131 cites W2035891227 @default.
- W2803682131 cites W2036464328 @default.
- W2803682131 cites W2040705798 @default.
- W2803682131 cites W2041883395 @default.
- W2803682131 cites W2045444923 @default.
- W2803682131 cites W2046425672 @default.
- W2803682131 cites W2050167683 @default.
- W2803682131 cites W2051135826 @default.
- W2803682131 cites W2058078579 @default.
- W2803682131 cites W2060836091 @default.
- W2803682131 cites W2061508611 @default.
- W2803682131 cites W2066625073 @default.
- W2803682131 cites W2068970107 @default.
- W2803682131 cites W2071952329 @default.
- W2803682131 cites W2077225298 @default.
- W2803682131 cites W2086323657 @default.
- W2803682131 cites W2092500470 @default.
- W2803682131 cites W2093021888 @default.
- W2803682131 cites W2094460294 @default.
- W2803682131 cites W2101701876 @default.
- W2803682131 cites W2105860371 @default.
- W2803682131 cites W2106216311 @default.
- W2803682131 cites W2106229058 @default.
- W2803682131 cites W2108762011 @default.
- W2803682131 cites W2115241110 @default.
- W2803682131 cites W2115519453 @default.
- W2803682131 cites W2115941371 @default.
- W2803682131 cites W2117467324 @default.
- W2803682131 cites W2123511348 @default.
- W2803682131 cites W2127568626 @default.
- W2803682131 cites W2127967394 @default.
- W2803682131 cites W2130265680 @default.
- W2803682131 cites W2131859934 @default.
- W2803682131 cites W2140126848 @default.
- W2803682131 cites W2141396945 @default.
- W2803682131 cites W2143453049 @default.
- W2803682131 cites W2144068357 @default.
- W2803682131 cites W2146668827 @default.
- W2803682131 cites W2148197128 @default.
- W2803682131 cites W2157433322 @default.
- W2803682131 cites W2158119672 @default.
- W2803682131 cites W2159714389 @default.
- W2803682131 cites W2166474344 @default.
- W2803682131 cites W2167456427 @default.
- W2803682131 cites W2167925521 @default.
- W2803682131 cites W2176028587 @default.
- W2803682131 cites W2238139779 @default.
- W2803682131 cites W2255950054 @default.
- W2803682131 cites W2298499289 @default.
- W2803682131 cites W2490493873 @default.
- W2803682131 cites W2734817281 @default.
- W2803682131 cites W2975741727 @default.
- W2803682131 cites W2976411930 @default.
- W2803682131 doi "https://doi.org/10.1016/j.ymben.2018.05.012" @default.
- W2803682131 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29842925" @default.
- W2803682131 hasPublicationYear "2018" @default.