FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2803713117> ?p ?o ?g. }

• W2803713117 endingPage "131" @default.
• W2803713117 startingPage "125" @default.
• W2803713117 abstract "Yarrowia lipolytica is an unconventional yeast that is regarded as safe. It is the potential platform for terpenoids production because it possesses the endogenous MVA pathway which can provide precursors for terpenoids synthesis. Herein, we constructed (+)-nootkatone, a sesquiterpenoid which is highly demanded in cosmetics and fragrance industries, synthetic pathway in Yarrowia lipolytica ATCC 201249. Heterologous production of (+)-nootkatone was achieved by co-expressing (+)-valencene synthase CnVS, codon-optimized (+)-nootkatone synthase opCYP706M1 and codon-optimized NADPH-cytochrome P450 reductase opAtCPR1. The initial (+)-nootkatone production was 45.6 μg/L. Fusion of opCYP706M1 and opt46AtCPR1 (opAtCPR1 with 46 amino acids truncated at N-terminal) increased (+)-valencene conversion efficiency to (+)-nootkatone and (+)-nootkatone production increased to 312.2 μg/L, nearly six times higher than the initial. Overexpression of the MVA pathway rate limiting enzymes 3-hydroxy-3-methylglutaryl-coenzyme A reductase tHMG1 and FPP synthase ERG20 improved the (+)-nootkatone production furtherly. The final engineered strain achieved a (+)-nootkatone titer of 978.2 μg/L, which was a 20.5-fold increase compared to those simply coexpressed CnVS, opCYP706M1 and opAtCPR1. This is the first report of heterologous biosynthesis of (+)-nootkatone in Y. lipolytica, which will provide a favorable reference for studies on heterologous production of other sesquiterpenoids and high-efficiency expression of P450 enzymes in Y. lipolytica." @default.
• W2803713117 created "2018-06-01" @default.
• W2803713117 creator A5002607713 @default.
• W2803713117 creator A5030831273 @default.
• W2803713117 creator A5045023952 @default.
• W2803713117 creator A5080932376 @default.
• W2803713117 date "2018-09-01" @default.
• W2803713117 modified "2023-10-13" @default.
• W2803713117 title "Heterologous biosynthesis of (+)-nootkatone in unconventional yeast Yarrowia lipolytica" @default.
• W2803713117 cites W1152640001 @default.
• W2803713117 cites W1732590418 @default.
• W2803713117 cites W190301388 @default.
• W2803713117 cites W1969002594 @default.
• W2803713117 cites W1971603746 @default.
• W2803713117 cites W1972272564 @default.
• W2803713117 cites W1972480206 @default.
• W2803713117 cites W2000811584 @default.
• W2803713117 cites W2009076519 @default.
• W2803713117 cites W2017353229 @default.
• W2803713117 cites W2017461184 @default.
• W2803713117 cites W2017805984 @default.
• W2803713117 cites W2020481873 @default.
• W2803713117 cites W2021699189 @default.
• W2803713117 cites W2024017173 @default.
• W2803713117 cites W2039790529 @default.
• W2803713117 cites W2048001922 @default.
• W2803713117 cites W2049548112 @default.
• W2803713117 cites W2058793466 @default.
• W2803713117 cites W2067453108 @default.
• W2803713117 cites W2074640918 @default.
• W2803713117 cites W2074779800 @default.
• W2803713117 cites W2076830784 @default.
• W2803713117 cites W2077275411 @default.
• W2803713117 cites W2077997117 @default.
• W2803713117 cites W2079953164 @default.
• W2803713117 cites W2086176935 @default.
• W2803713117 cites W2087383176 @default.
• W2803713117 cites W2089992379 @default.
• W2803713117 cites W2106951530 @default.
• W2803713117 cites W2114527818 @default.
• W2803713117 cites W2114570899 @default.
• W2803713117 cites W2152632558 @default.
• W2803713117 cites W2160556993 @default.
• W2803713117 cites W2185639583 @default.
• W2803713117 cites W2239360447 @default.
• W2803713117 cites W2287453937 @default.
• W2803713117 cites W2294225971 @default.
• W2803713117 cites W2316990088 @default.
• W2803713117 cites W2335457124 @default.
• W2803713117 cites W2408769325 @default.
• W2803713117 cites W2530603323 @default.
• W2803713117 cites W2563320377 @default.
• W2803713117 cites W2596643790 @default.
• W2803713117 cites W2606736518 @default.
• W2803713117 cites W2630017275 @default.
• W2803713117 cites W88189612 @default.
• W2803713117 cites W99972145 @default.
• W2803713117 doi "https://doi.org/10.1016/j.bej.2018.05.023" @default.
• W2803713117 hasPublicationYear "2018" @default.
• W2803713117 type Work @default.
• W2803713117 sameAs 2803713117 @default.
• W2803713117 citedByCount "60" @default.
• W2803713117 countsByYear W28037131172018 @default.
• W2803713117 countsByYear W28037131172019 @default.
• W2803713117 countsByYear W28037131172020 @default.
• W2803713117 countsByYear W28037131172021 @default.
• W2803713117 countsByYear W28037131172022 @default.
• W2803713117 countsByYear W28037131172023 @default.
• W2803713117 crossrefType "journal-article" @default.
• W2803713117 hasAuthorship W2803713117A5002607713 @default.
• W2803713117 hasAuthorship W2803713117A5030831273 @default.
• W2803713117 hasAuthorship W2803713117A5045023952 @default.
• W2803713117 hasAuthorship W2803713117A5080932376 @default.
• W2803713117 hasConcept C104317684 @default.
• W2803713117 hasConcept C108305142 @default.
• W2803713117 hasConcept C134651460 @default.
• W2803713117 hasConcept C181199279 @default.
• W2803713117 hasConcept C185592680 @default.
• W2803713117 hasConcept C206103511 @default.
• W2803713117 hasConcept C2775933503 @default.
• W2803713117 hasConcept C2779222958 @default.
• W2803713117 hasConcept C2779490540 @default.
• W2803713117 hasConcept C2779982574 @default.
• W2803713117 hasConcept C2780115692 @default.
• W2803713117 hasConcept C40767141 @default.
• W2803713117 hasConcept C553450214 @default.
• W2803713117 hasConcept C55493867 @default.
• W2803713117 hasConcept C6350086 @default.
• W2803713117 hasConceptScore W2803713117C104317684 @default.
• W2803713117 hasConceptScore W2803713117C108305142 @default.
• W2803713117 hasConceptScore W2803713117C134651460 @default.
• W2803713117 hasConceptScore W2803713117C181199279 @default.
• W2803713117 hasConceptScore W2803713117C185592680 @default.
• W2803713117 hasConceptScore W2803713117C206103511 @default.
• W2803713117 hasConceptScore W2803713117C2775933503 @default.
• W2803713117 hasConceptScore W2803713117C2779222958 @default.
• W2803713117 hasConceptScore W2803713117C2779490540 @default.
• W2803713117 hasConceptScore W2803713117C2779982574 @default.

• next