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- W2803734153 abstract "CRISPR/dCas9 binds precisely to defined genomic sequences through targeting of guide RNA (gRNA) sequences. In vivo imaging of genomic loci can be achieved by recruiting fluorescent proteins using either dCas9 or gRNA. We thoroughly validate and compare the effectiveness and specificity of several dCas9/gRNA genome labeling systems. Surprisingly, we discover that in the gRNA-labeling strategies, accumulation of tagged gRNA transcripts leads to non-specific labeling foci. Furthermore, we develop novel bimolecular fluorescence complementation (BIFC) methods that combine the advantages of both dCas9-labeling and gRNA-labeling strategies. The BIFC-dCas9/gRNA methods demonstrate high signal-to-noise ratios and have no non-specific foci." @default.
- W2803734153 created "2018-06-01" @default.
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- W2803734153 date "2018-03-22" @default.
- W2803734153 modified "2023-10-16" @default.
- W2803734153 title "Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging" @default.
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- W2803734153 doi "https://doi.org/10.1186/s13059-018-1413-5" @default.
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