Matches in SemOpenAlex for { <https://semopenalex.org/work/W280375296> ?p ?o ?g. }
- W280375296 endingPage "78" @default.
- W280375296 startingPage "70" @default.
- W280375296 abstract "In addition to cellulose and hemicellulose, pectin is an important renewable resource for biorefinery. Endo- and exo-polygalacturonases are two essential enzymes required for complete deconstruction of pectin into simple and fermentable sugars. In biorefinery, thermophilic pectinases are more appealing than their mesophilic counterparts. Two pectinase genes encoding a putative exo-polygalacturonase (NfPG4) and a putative endopolygalacturonase (NfPG5), respectively, were thus cloned from a thermophilic filamentous fungus Neosartorya fischeri P1 and successfully expressed in Pichia pastoris. NfPG4 and NfPG5 share 79% and 58% identity, respectively, with their closest characterized homologs from Aspergillus tubingensis and Achaetomium sp. Xz-8. They are both acidic and thermophilic, with maximum activities at pH 3.5 and 65 °C for NfPG4 and pH 4.5 and 70 °C for NfPG5, respectively. In comparison with other characterized thermophilic polygalacturonases, NfPG5 is the most thermophilic fungal endopolygalacturonase. Both enzymes have excellent stability at 55 °C and over a broad pH range (pH 3.0–8.0 for NfPG4 and pH 2.0–11.0 for NfPG5), and are strongly resistant to many metal ions and chemical reagents. NfPG5 has a typical endo-mode of action on polygalacturonic acid, releasing galacturonic acid, di-galacturonic acid, and tri-galacturonic acid. In contrast, NfPG4 only releases galacturonic acid as the end hydrolysis product, typified by exo-polygalacturonases. NfPG4 has a specific activity of 700.9 U/mg, higher than most characterized exo-polygalacturonases. NfPG5 is also a robust polygalacturonase with a specific activity of 3630.4 U/mg. These superior properties make the two polygalacturonases interesting candidates for industrial applications with a preference for thermophilic pectinases." @default.
- W280375296 created "2016-06-24" @default.
- W280375296 creator A5005110304 @default.
- W280375296 creator A5008371533 @default.
- W280375296 creator A5052310341 @default.
- W280375296 creator A5056550372 @default.
- W280375296 creator A5063103575 @default.
- W280375296 creator A5066858473 @default.
- W280375296 creator A5067418759 @default.
- W280375296 creator A5091317996 @default.
- W280375296 date "2015-08-01" @default.
- W280375296 modified "2023-10-16" @default.
- W280375296 title "Two thermophilic fungal pectinases from Neosartorya fischeri P1: Gene cloning, expression, and biochemical characterization" @default.
- W280375296 cites W1546656845 @default.
- W280375296 cites W1972876873 @default.
- W280375296 cites W1984370122 @default.
- W280375296 cites W1989256337 @default.
- W280375296 cites W1993521456 @default.
- W280375296 cites W2005685893 @default.
- W280375296 cites W2009674410 @default.
- W280375296 cites W2011579361 @default.
- W280375296 cites W2013105984 @default.
- W280375296 cites W2015064119 @default.
- W280375296 cites W2015247304 @default.
- W280375296 cites W2016822801 @default.
- W280375296 cites W2021070035 @default.
- W280375296 cites W2021654888 @default.
- W280375296 cites W2028235860 @default.
- W280375296 cites W2029330674 @default.
- W280375296 cites W2030028996 @default.
- W280375296 cites W2030394993 @default.
- W280375296 cites W2032811755 @default.
- W280375296 cites W2038157485 @default.
- W280375296 cites W2040470885 @default.
- W280375296 cites W2044637892 @default.
- W280375296 cites W2045904102 @default.
- W280375296 cites W2048855401 @default.
- W280375296 cites W2057984012 @default.
- W280375296 cites W2073970735 @default.
- W280375296 cites W2081522403 @default.
- W280375296 cites W2096064392 @default.
- W280375296 cites W2100837269 @default.
- W280375296 cites W2101421252 @default.
- W280375296 cites W2104863787 @default.
- W280375296 cites W2108179651 @default.
- W280375296 cites W2110695343 @default.
- W280375296 cites W2132629607 @default.
- W280375296 cites W2139633429 @default.
- W280375296 cites W2144876083 @default.
- W280375296 cites W2147688972 @default.
- W280375296 cites W2149369491 @default.
- W280375296 cites W2153195262 @default.
- W280375296 cites W2166989211 @default.
- W280375296 cites W2168506412 @default.
- W280375296 cites W2348537412 @default.
- W280375296 cites W4293247451 @default.
- W280375296 cites W567322843 @default.
- W280375296 doi "https://doi.org/10.1016/j.molcatb.2015.05.005" @default.
- W280375296 hasPublicationYear "2015" @default.
- W280375296 type Work @default.
- W280375296 sameAs 280375296 @default.
- W280375296 citedByCount "12" @default.
- W280375296 countsByYear W2803752962016 @default.
- W280375296 countsByYear W2803752962017 @default.
- W280375296 countsByYear W2803752962018 @default.
- W280375296 countsByYear W2803752962021 @default.
- W280375296 countsByYear W2803752962022 @default.
- W280375296 countsByYear W2803752962023 @default.
- W280375296 crossrefType "journal-article" @default.
- W280375296 hasAuthorship W280375296A5005110304 @default.
- W280375296 hasAuthorship W280375296A5008371533 @default.
- W280375296 hasAuthorship W280375296A5052310341 @default.
- W280375296 hasAuthorship W280375296A5056550372 @default.
- W280375296 hasAuthorship W280375296A5063103575 @default.
- W280375296 hasAuthorship W280375296A5066858473 @default.
- W280375296 hasAuthorship W280375296A5067418759 @default.
- W280375296 hasAuthorship W280375296A5091317996 @default.
- W280375296 hasConcept C104317684 @default.
- W280375296 hasConcept C172854840 @default.
- W280375296 hasConcept C181199279 @default.
- W280375296 hasConcept C185592680 @default.
- W280375296 hasConcept C2775859485 @default.
- W280375296 hasConcept C2779920640 @default.
- W280375296 hasConcept C2780756937 @default.
- W280375296 hasConcept C2781372317 @default.
- W280375296 hasConcept C27911776 @default.
- W280375296 hasConcept C40767141 @default.
- W280375296 hasConcept C55493867 @default.
- W280375296 hasConcept C86803240 @default.
- W280375296 hasConceptScore W280375296C104317684 @default.
- W280375296 hasConceptScore W280375296C172854840 @default.
- W280375296 hasConceptScore W280375296C181199279 @default.
- W280375296 hasConceptScore W280375296C185592680 @default.
- W280375296 hasConceptScore W280375296C2775859485 @default.
- W280375296 hasConceptScore W280375296C2779920640 @default.
- W280375296 hasConceptScore W280375296C2780756937 @default.
- W280375296 hasConceptScore W280375296C2781372317 @default.
- W280375296 hasConceptScore W280375296C27911776 @default.