FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2803811287> ?p ?o ?g. }

• W2803811287 abstract "Porcine reproductive and respiratory syndrome virus (PRRSV), a virulent pathogen of swine, suppresses the innate immune response and induces persistent infection. One mechanism used by viruses to evade the immune system is to cripple the antigen-processing machinery in monocyte-derived dendritic cells (MoDCs). In this study, we show that MoDCs infected by PRRSV express lower levels of the major histocompatibility complex (MHC)-peptide complex proteins TAP1 and ERp57 and are impaired in their ability to stimulate T cell proliferation and increase their production of CD83. Neutralization of sCD83 removes the inhibitory effects of PRRSV on MoDCs. When MoDCs are incubated with exogenously added sCD83 protein, TAP1 and ERp57 expression decreases and T lymphocyte activation is impaired. PRRSV nonstructural protein 1α (Nsp1α) enhances CD83 promoter activity. Mutations in the ZF domain of Nsp1α abolish its ability to activate the CD83 promoter. We generated recombinant PRRSVs with mutations in Nsp1α and the corresponding repaired PRRSVs. Viruses with Nsp1α mutations did not decrease levels of TAP1 and ERp57, impair the ability of MoDCs to stimulate T cell proliferation, or increase levels of sCD83. We show that the ZF domain of Nsp1α stimulates the secretion of CD83, which in turn inhibits MoDC function. Our study provides new insights into the mechanisms of immune suppression by PRRSV.IMPORTANCE PRRSV has a severe impact on the swine industry throughout the world. Understanding the mechanisms by which PRRSV infection suppresses the immune system is essential for a robust and sustainable swine industry. Here, we demonstrated that PRRSV infection manipulates MoDCs by interfering with their ability to produce proteins in the MHC-peptide complex. The virus also impairs the ability of MoDCs to stimulate cell proliferation, due in large part to the enhanced release of soluble CD83 from PRRSV-infected MoDCs. The viral nonstructural protein 1 (Nsp1) is responsible for upregulating CD83 promoter activity. Amino acids in the ZF domain of Nsp1α (L5-2A, rG45A, G48A, and L61-6A) are essential for CD83 promoter activation. Viruses with mutations at these sites no longer inhibit MoDC-mediated T cell proliferation. These findings provide novel insights into the mechanism by which the adaptive immune response is suppressed during PRRSV infection." @default.
• W2803811287 created "2018-06-01" @default.
• W2803811287 creator A5005602487 @default.
• W2803811287 creator A5008171524 @default.
• W2803811287 creator A5019383992 @default.
• W2803811287 creator A5022995935 @default.
• W2803811287 creator A5030649241 @default.
• W2803811287 creator A5030929816 @default.
• W2803811287 creator A5084705624 @default.
• W2803811287 date "2018-08-01" @default.
• W2803811287 modified "2023-10-10" @default.
• W2803811287 title "Nsp1α of Porcine Reproductive and Respiratory Syndrome Virus Strain BB0907 Impairs the Function of Monocyte-Derived Dendritic Cells via the Release of Soluble CD83" @default.
• W2803811287 cites W1543299964 @default.
• W2803811287 cites W1590347389 @default.
• W2803811287 cites W1939967389 @default.
• W2803811287 cites W1989478386 @default.
• W2803811287 cites W1996686270 @default.
• W2803811287 cites W1997733459 @default.
• W2803811287 cites W1999933198 @default.
• W2803811287 cites W2000817149 @default.
• W2803811287 cites W2002492535 @default.
• W2803811287 cites W2002899213 @default.
• W2803811287 cites W2002987613 @default.
• W2803811287 cites W2010084996 @default.
• W2803811287 cites W2011130664 @default.
• W2803811287 cites W2020555122 @default.
• W2803811287 cites W2021008297 @default.
• W2803811287 cites W2053111450 @default.
• W2803811287 cites W2062804795 @default.
• W2803811287 cites W2066259193 @default.
• W2803811287 cites W2066978505 @default.
• W2803811287 cites W2067481135 @default.
• W2803811287 cites W2071636227 @default.
• W2803811287 cites W2071700214 @default.
• W2803811287 cites W2079278536 @default.
• W2803811287 cites W2087922624 @default.
• W2803811287 cites W2088856300 @default.
• W2803811287 cites W2089278121 @default.
• W2803811287 cites W2092463285 @default.
• W2803811287 cites W2099818654 @default.
• W2803811287 cites W2113657154 @default.
• W2803811287 cites W2114174897 @default.
• W2803811287 cites W2116255606 @default.
• W2803811287 cites W2126339695 @default.
• W2803811287 cites W2129291354 @default.
• W2803811287 cites W2131473413 @default.
• W2803811287 cites W2150178919 @default.
• W2803811287 cites W2158335449 @default.
• W2803811287 cites W2159132427 @default.
• W2803811287 cites W2235022660 @default.
• W2803811287 cites W2238107379 @default.
• W2803811287 cites W2251775966 @default.
• W2803811287 cites W2261250902 @default.
• W2803811287 cites W2280795966 @default.
• W2803811287 cites W2335831020 @default.
• W2803811287 cites W2417033504 @default.
• W2803811287 cites W2524070553 @default.
• W2803811287 cites W2528828967 @default.
• W2803811287 cites W2539522951 @default.
• W2803811287 cites W2545397852 @default.
• W2803811287 cites W2559388670 @default.
• W2803811287 cites W2583176331 @default.
• W2803811287 cites W2593167304 @default.
• W2803811287 cites W2617697313 @default.
• W2803811287 cites W2627077408 @default.
• W2803811287 cites W2730491138 @default.
• W2803811287 cites W2734646604 @default.
• W2803811287 cites W2735892656 @default.
• W2803811287 cites W2744206077 @default.
• W2803811287 cites W2754195618 @default.
• W2803811287 cites W2754324083 @default.
• W2803811287 cites W2761584116 @default.
• W2803811287 cites W2761951023 @default.
• W2803811287 cites W2763995920 @default.
• W2803811287 cites W2766547496 @default.
• W2803811287 cites W4211001041 @default.
• W2803811287 cites W652862567 @default.
• W2803811287 cites W970789458 @default.
• W2803811287 doi "https://doi.org/10.1128/jvi.00366-18" @default.
• W2803811287 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6052304" @default.
• W2803811287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29793955" @default.
• W2803811287 hasPublicationYear "2018" @default.
• W2803811287 type Work @default.
• W2803811287 sameAs 2803811287 @default.
• W2803811287 citedByCount "11" @default.
• W2803811287 countsByYear W28038112872019 @default.
• W2803811287 countsByYear W28038112872021 @default.
• W2803811287 countsByYear W28038112872022 @default.
• W2803811287 countsByYear W28038112872023 @default.
• W2803811287 crossrefType "journal-article" @default.
• W2803811287 hasAuthorship W2803811287A5005602487 @default.
• W2803811287 hasAuthorship W2803811287A5008171524 @default.
• W2803811287 hasAuthorship W2803811287A5019383992 @default.
• W2803811287 hasAuthorship W2803811287A5022995935 @default.
• W2803811287 hasAuthorship W2803811287A5030649241 @default.
• W2803811287 hasAuthorship W2803811287A5030929816 @default.
• W2803811287 hasAuthorship W2803811287A5084705624 @default.
• W2803811287 hasBestOaLocation W28038112871 @default.
• W2803811287 hasConcept C136449434 @default.
• W2803811287 hasConcept C159047783 @default.

• next