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• W2803895699 abstract "To fully actualize artificial, cell-laden biological models in tissue engineering, such as 3D organoids and organs-on-a-chip systems, cells need to be patterned such that they can precisely mimic natural microenvironments in vitro. Despite increasing interest in this area, patterning cells at multiscale (∼10 μm to 10 mm) remains a significant challenge in bioengineering. Here, we report a projection-based 3D printing system that achieves rapid and high-resolution fabrication of hydrogel scaffolds featuring intricate channels for multiscale cell patterning. Using this system, we were able to use biocompatible poly(ethylene glycol)diacrylate in fabricating a variety of scaffold architectures, ranging from regular geometries such as serpentine, spiral, and fractal-like to more irregular/intricate geometries, such as biomimetic arborescent and capillary networks. A red food dye solution was able to freely fill all channels in the scaffolds, from the trunk (>1100 μm in width) to the small branch (∼17 μm in width) without an external pump. The dimensions of the printed scaffolds remained stable over 3 days while being immersed in Dulbecco’s phosphate-buffered saline at 37 °C, and a penetration analysis revealed that these scaffolds are suitable for metabolic and nutrient transport. Cell patterning experiments showed that red fluorescent protein-transfected A549 human nonsmall lung cancer cells adhered well in the scaffolds’ channels, and showed further attachment and penetration during cell culture proliferation." @default.
• W2803895699 created "2018-06-01" @default.
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• W2803895699 date "2018-05-21" @default.
• W2803895699 modified "2023-10-05" @default.
• W2803895699 title "Projection-Based 3D Printing of Cell Patterning Scaffolds with Multiscale Channels" @default.
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• W2803895699 doi "https://doi.org/10.1021/acsami.8b03867" @default.
• W2803895699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29782142" @default.
• W2803895699 hasPublicationYear "2018" @default.
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