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- W2804091118 abstract "Multiple antigenic peptide (MAP) vaccines have advantages over traditional Toxoplasma gondii vaccines, but are more susceptible to enzymatic degradation. As an effective delivery system, chitosan microspheres (CS) can overcome this obstacle and act as a natural adjuvant to promote T helper 1 (Th1) cellular immune responses. In this study, we use chitosan microparticles to deliver multiple antigenic epitopes from GRA10 (G10E), containing three dominant epitopes. When G10E was entrapped within chitosan microparticles (G10E-CS), adequate peptides for eliciting immune response were loaded in the microsphere core and this complex released G10E peptides stably. The efficiency of G10E-CS was detected both in vitro, via cell culture, and through in vivo mouse immunization. In vitro, G10E-CS activated Dendritic Cells (DC) and T lymphocytes by upregulating the secretion of costimulatory molecules (CD40 and CD86). In vivo, Th1 biased cellular and humoral immune responses were activated in mice vaccinated with G10E-CS, accompanied by significantly increased production of IFN-γ, IL-2 and IgG2a, and decreases in IL-4, IL-10 and IgG1. Immunization with G10E-CS conferred significant protection with prolonged survival in mice model of acute toxoplasmosis and statistically significant decreases in cyst burden in murine chronic toxoplasmosis. The results from this study indicate that chitosan microspheres used as an effective system to deliver a linked antigenic peptides is a promising strategy for the development of efficient vaccine against T. gondii." @default.
- W2804091118 created "2018-06-01" @default.
- W2804091118 creator A5009041393 @default.
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- W2804091118 date "2018-05-23" @default.
- W2804091118 modified "2023-10-16" @default.
- W2804091118 title "Chitosan Microsphere Used as an Effective System to Deliver a Linked Antigenic Peptides Vaccine Protect Mice Against Acute and Chronic Toxoplasmosis" @default.
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- W2804091118 doi "https://doi.org/10.3389/fcimb.2018.00163" @default.
- W2804091118 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5974094" @default.
- W2804091118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29876322" @default.
- W2804091118 hasPublicationYear "2018" @default.