Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804097999> ?p ?o ?g. }
- W2804097999 endingPage "382" @default.
- W2804097999 startingPage "371" @default.
- W2804097999 abstract "Microglial cells as innate immune key players have a critical and unique role in neurodegenerative disorders. They strongly interact with their microenvironment in a complex manner and react to changes by switching their phenotype and functional activation states. In order to understand the development of brain diseases, it is imperative to elucidate up- or down-regulation of genes involved in microglia polarisation in time-profile by a simple-to-use strategy. Here, we present a new imaging strategy to follow promoter activity of genes involved in microglia polarisation. We lentivirally transduced BV-2 microglia cells in culture with constructs consisting of the induced nitric oxide synthase (iNOS), Fc gamma receptor III (Fcgr3) (both resembling the pro-inflammatory M1-like phenotype) or Chitinase-like 3 (Chil3/Ym1) (resembling the anti-inflammatory M2-like phenotype) promoters and stimulated transgenic cells with potent activators for pro- or anti-inflammatory response, such as lipopolysaccharide (LPS) + interferon gamma (IFN-γ) or interleukin (IL)-4, respectively. Promoter activities upon polarisation phases were quantitatively assessed by the two imaging reporters Luc2 for bioluminescence and eGFP for fluorescence." @default.
- W2804097999 created "2018-06-01" @default.
- W2804097999 creator A5005807034 @default.
- W2804097999 creator A5013586280 @default.
- W2804097999 creator A5015543792 @default.
- W2804097999 creator A5022630592 @default.
- W2804097999 creator A5023429269 @default.
- W2804097999 creator A5071237549 @default.
- W2804097999 creator A5079336621 @default.
- W2804097999 date "2018-05-22" @default.
- W2804097999 modified "2023-10-17" @default.
- W2804097999 title "Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation" @default.
- W2804097999 cites W1556188925 @default.
- W2804097999 cites W1768846296 @default.
- W2804097999 cites W1972688728 @default.
- W2804097999 cites W1986944486 @default.
- W2804097999 cites W2005979110 @default.
- W2804097999 cites W2011361113 @default.
- W2804097999 cites W2011984976 @default.
- W2804097999 cites W2018521741 @default.
- W2804097999 cites W2036439277 @default.
- W2804097999 cites W2042852723 @default.
- W2804097999 cites W2045490045 @default.
- W2804097999 cites W2050283646 @default.
- W2804097999 cites W2055065148 @default.
- W2804097999 cites W2057745345 @default.
- W2804097999 cites W2058669152 @default.
- W2804097999 cites W2086542713 @default.
- W2804097999 cites W2097794242 @default.
- W2804097999 cites W2101898118 @default.
- W2804097999 cites W2107956674 @default.
- W2804097999 cites W2108663777 @default.
- W2804097999 cites W2134729461 @default.
- W2804097999 cites W2140380746 @default.
- W2804097999 cites W2147180300 @default.
- W2804097999 cites W2149200346 @default.
- W2804097999 cites W2170959966 @default.
- W2804097999 cites W2209250550 @default.
- W2804097999 cites W2346184067 @default.
- W2804097999 cites W2418294955 @default.
- W2804097999 cites W2430164892 @default.
- W2804097999 cites W2514708421 @default.
- W2804097999 cites W2586609554 @default.
- W2804097999 cites W2759112119 @default.
- W2804097999 cites W4249736906 @default.
- W2804097999 doi "https://doi.org/10.1007/s11481-018-9789-2" @default.
- W2804097999 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6096558" @default.
- W2804097999 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29790106" @default.
- W2804097999 hasPublicationYear "2018" @default.
- W2804097999 type Work @default.
- W2804097999 sameAs 2804097999 @default.
- W2804097999 citedByCount "2" @default.
- W2804097999 countsByYear W28040979992019 @default.
- W2804097999 crossrefType "journal-article" @default.
- W2804097999 hasAuthorship W2804097999A5005807034 @default.
- W2804097999 hasAuthorship W2804097999A5013586280 @default.
- W2804097999 hasAuthorship W2804097999A5015543792 @default.
- W2804097999 hasAuthorship W2804097999A5022630592 @default.
- W2804097999 hasAuthorship W2804097999A5023429269 @default.
- W2804097999 hasAuthorship W2804097999A5071237549 @default.
- W2804097999 hasAuthorship W2804097999A5079336621 @default.
- W2804097999 hasBestOaLocation W28040979991 @default.
- W2804097999 hasConcept C102230213 @default.
- W2804097999 hasConcept C104317684 @default.
- W2804097999 hasConcept C127716648 @default.
- W2804097999 hasConcept C136449434 @default.
- W2804097999 hasConcept C169760540 @default.
- W2804097999 hasConcept C185592680 @default.
- W2804097999 hasConcept C203014093 @default.
- W2804097999 hasConcept C24998067 @default.
- W2804097999 hasConcept C2776914184 @default.
- W2804097999 hasConcept C2778754761 @default.
- W2804097999 hasConcept C2779830541 @default.
- W2804097999 hasConcept C54355233 @default.
- W2804097999 hasConcept C86803240 @default.
- W2804097999 hasConcept C8891405 @default.
- W2804097999 hasConcept C95444343 @default.
- W2804097999 hasConceptScore W2804097999C102230213 @default.
- W2804097999 hasConceptScore W2804097999C104317684 @default.
- W2804097999 hasConceptScore W2804097999C127716648 @default.
- W2804097999 hasConceptScore W2804097999C136449434 @default.
- W2804097999 hasConceptScore W2804097999C169760540 @default.
- W2804097999 hasConceptScore W2804097999C185592680 @default.
- W2804097999 hasConceptScore W2804097999C203014093 @default.
- W2804097999 hasConceptScore W2804097999C24998067 @default.
- W2804097999 hasConceptScore W2804097999C2776914184 @default.
- W2804097999 hasConceptScore W2804097999C2778754761 @default.
- W2804097999 hasConceptScore W2804097999C2779830541 @default.
- W2804097999 hasConceptScore W2804097999C54355233 @default.
- W2804097999 hasConceptScore W2804097999C86803240 @default.
- W2804097999 hasConceptScore W2804097999C8891405 @default.
- W2804097999 hasConceptScore W2804097999C95444343 @default.
- W2804097999 hasFunder F4320338362 @default.
- W2804097999 hasIssue "3" @default.
- W2804097999 hasLocation W28040979991 @default.
- W2804097999 hasLocation W28040979992 @default.
- W2804097999 hasLocation W28040979993 @default.
- W2804097999 hasLocation W28040979994 @default.