Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804137044> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2804137044 abstract "The aim of the present study was to investigate the effect of co‑culture with amniotic epithelial cells (AECs) on the biological characteristics of amniotic mesenchymal stem cells (AMSCs), to compare the expression of C‑X‑C motif chemokine receptor 4 (CXCR4) in co‑cultured AMSCs and to investigate the roles of the stromal cell‑derived factor‑1 (SDF‑1)/CXCR4 axis in the homing and migration of AMSCs. AMSCs were isolated from human amniotic membranes, purified and then differentiated into osteoblasts and adipocytes in vitro, which was verified by von Kossa Staining and Oil Red O staining. Cell viability was measured by Cell Counting kit‑8 and trypan blue assays at 24, 48 and 72 h, the expression of CXCR4 was analyzed by immunofluorescence‑based flow cytometry and reverse transcription‑quantitative polymerase chain reaction, and the migration ability of AMSCs in vitro was observed by a migration assay. The results demonstrated that cell viability (at 48 and 72 h) and survival (at 24, 48 and 72 h) in the co‑culture and serum groups were higher compared with the serum‑free group. Furthermore, CXCR4 mRNA and protein expression, and migration along the SDF‑1 gradient, in the co‑culture and serum‑free groups were higher compared with the serum group. Overall, the results indicated that AMSCs co‑cultured with AECs exhibited enhanced proliferation activity and survival rate. In conclusion, the present study demonstrated that co‑culture of AMSCs with AECs upregulated CXCR4 on the surface of AMSCs and enhanced the migration ability of AMSCs in vitro. This result may improve the directional migration and homing ability of AMSCs, as well as provide a theoretical basis for the application of AMSCs in clinical practice as a novel strategy to increase the success of hematopoietic stem cell transplantation." @default.
- W2804137044 created "2018-06-01" @default.
- W2804137044 creator A5011980528 @default.
- W2804137044 creator A5022814889 @default.
- W2804137044 creator A5023019679 @default.
- W2804137044 creator A5035760896 @default.
- W2804137044 creator A5045573843 @default.
- W2804137044 creator A5051832254 @default.
- W2804137044 creator A5068136036 @default.
- W2804137044 creator A5077375928 @default.
- W2804137044 date "2018-05-23" @default.
- W2804137044 modified "2023-09-27" @default.
- W2804137044 title "Effect of co‑culture with amniotic epithelial cells on the biological characteristics of amniotic mesenchymal stem cells" @default.
- W2804137044 doi "https://doi.org/10.3892/mmr.2018.9053" @default.
- W2804137044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29845205" @default.
- W2804137044 hasPublicationYear "2018" @default.
- W2804137044 type Work @default.
- W2804137044 sameAs 2804137044 @default.
- W2804137044 citedByCount "7" @default.
- W2804137044 countsByYear W28041370442019 @default.
- W2804137044 countsByYear W28041370442020 @default.
- W2804137044 countsByYear W28041370442021 @default.
- W2804137044 countsByYear W28041370442022 @default.
- W2804137044 crossrefType "journal-article" @default.
- W2804137044 hasAuthorship W2804137044A5011980528 @default.
- W2804137044 hasAuthorship W2804137044A5022814889 @default.
- W2804137044 hasAuthorship W2804137044A5023019679 @default.
- W2804137044 hasAuthorship W2804137044A5035760896 @default.
- W2804137044 hasAuthorship W2804137044A5045573843 @default.
- W2804137044 hasAuthorship W2804137044A5051832254 @default.
- W2804137044 hasAuthorship W2804137044A5068136036 @default.
- W2804137044 hasAuthorship W2804137044A5077375928 @default.
- W2804137044 hasBestOaLocation W28041370441 @default.
- W2804137044 hasConcept C129470790 @default.
- W2804137044 hasConcept C13373296 @default.
- W2804137044 hasConcept C153911025 @default.
- W2804137044 hasConcept C16685009 @default.
- W2804137044 hasConcept C16930146 @default.
- W2804137044 hasConcept C198826908 @default.
- W2804137044 hasConcept C203014093 @default.
- W2804137044 hasConcept C28328180 @default.
- W2804137044 hasConcept C502942594 @default.
- W2804137044 hasConcept C553184892 @default.
- W2804137044 hasConcept C71924100 @default.
- W2804137044 hasConcept C86803240 @default.
- W2804137044 hasConcept C8891405 @default.
- W2804137044 hasConcept C95444343 @default.
- W2804137044 hasConceptScore W2804137044C129470790 @default.
- W2804137044 hasConceptScore W2804137044C13373296 @default.
- W2804137044 hasConceptScore W2804137044C153911025 @default.
- W2804137044 hasConceptScore W2804137044C16685009 @default.
- W2804137044 hasConceptScore W2804137044C16930146 @default.
- W2804137044 hasConceptScore W2804137044C198826908 @default.
- W2804137044 hasConceptScore W2804137044C203014093 @default.
- W2804137044 hasConceptScore W2804137044C28328180 @default.
- W2804137044 hasConceptScore W2804137044C502942594 @default.
- W2804137044 hasConceptScore W2804137044C553184892 @default.
- W2804137044 hasConceptScore W2804137044C71924100 @default.
- W2804137044 hasConceptScore W2804137044C86803240 @default.
- W2804137044 hasConceptScore W2804137044C8891405 @default.
- W2804137044 hasConceptScore W2804137044C95444343 @default.
- W2804137044 hasLocation W28041370441 @default.
- W2804137044 hasLocation W28041370442 @default.
- W2804137044 hasOpenAccess W2804137044 @default.
- W2804137044 hasPrimaryLocation W28041370441 @default.
- W2804137044 hasRelatedWork W1987109479 @default.
- W2804137044 hasRelatedWork W1994856171 @default.
- W2804137044 hasRelatedWork W1997743486 @default.
- W2804137044 hasRelatedWork W2007331748 @default.
- W2804137044 hasRelatedWork W2024745401 @default.
- W2804137044 hasRelatedWork W2029025070 @default.
- W2804137044 hasRelatedWork W2045204889 @default.
- W2804137044 hasRelatedWork W2054861783 @default.
- W2804137044 hasRelatedWork W2058122659 @default.
- W2804137044 hasRelatedWork W2058781217 @default.
- W2804137044 hasRelatedWork W2105445775 @default.
- W2804137044 hasRelatedWork W2148064846 @default.
- W2804137044 hasRelatedWork W2160597506 @default.
- W2804137044 hasRelatedWork W2331913619 @default.
- W2804137044 hasRelatedWork W2347250184 @default.
- W2804137044 hasRelatedWork W2435876996 @default.
- W2804137044 hasRelatedWork W2914800204 @default.
- W2804137044 hasRelatedWork W327830853 @default.
- W2804137044 hasRelatedWork W43219361 @default.
- W2804137044 hasRelatedWork W2277361594 @default.
- W2804137044 isParatext "false" @default.
- W2804137044 isRetracted "false" @default.
- W2804137044 magId "2804137044" @default.
- W2804137044 workType "article" @default.