FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804229132> ?p ?o ?g. }

• W2804229132 abstract "A total of 221 pili accessions, including the 7 NSIC-registered varieties, have been collected from the germplasm collections of IPB-CSC [Institute of Plant Breeding-Crop Science Cluster], CSC orchard (Pili Drive) and DA [Department of Agriculture] Pili Research and Technology Center (DA-PRTC) in Tabaco, Albay [Philippines], as well as from three private pili farms in Bulusan, Sorsogon [Philippines]. Fruit (whole fruit, pulp, nut, shell, kernel) characters have been evaluated for each pili accession according to the tropical fruit descriptor set by the International Board for Plant Genetic Resources (IBGRI) with 'Katutubo' as reference variety. The pili accessions were ranked for fruit character evaluated and several accessions, mostly from Pili Drive, were identified to be superior to 'Katutubo' in terms of fruit characters. Furthermore, tree characters (growth habit, dwarfness, leaf shape, growth and vigor, fruit setting) have been evaluated for all trees except for 17 accessions from the DA-PRTC. SSR [simple sequence repeat] marker primers which have been successfully used in papaya and published primers used in Canarium album (Zhang et al., 2009) were used for cross-species amplification in pili. Six out of the 12 polymorphic primer pairs screened have been optimized and used for genetic diversity analysis and DNA fingerprinting for variety identification of pili. For genetic diversity analysis, five SSR markers amplified a total of 24 alleles (4.8 alleles per markers) in the 95 pili accessions. The average PIC of the five markers was 0.6987 indicating the capability of the primers to detect and quantify genetic diversity in C. ovatum. The initial DNA fingerprints of the 7 NSIC-registered pili varieties and Canarium luzonicum using 6 SSR primers have been established: Katutubo (111111), Lanuza (112212), Laysa (223322), Magayon (114411), Magnaye (104511), Mayon I (115612), Orolfo (114711), and C. luzonicum (326812). Each digit of the finger print refers to the banding patterns for markers Cas A 131, Cas A 183, Cas C 120, SSR 12, SSR 31 and SSR 38, respectively. The fingerprints were informative and differences were observed among the genotypes in terms of the uniqueness of identification numbers with the 6 primers. This demonstrated the usefulness of this technique for varietal identification in pili. Moreover, the dendrogram generated based on SAHN cluster analysis using UPGMA revealed that the varieties and C. luzonicum formed two groups at 0.60 coefficient of similarity namely: Group 1 - Katutubo, Magayon, Magnaye, Orolfo and Laysa and Group 2 – Lanuza, Mayon 1 and C. luzonicum. However for Group 2, at 0.75 coefficient of similarity, C. luzonicum separates from the C. ovatum varieties. A partial cDNA library of pili was prepared. The full length cDNAs of a small heat shock protein (583 bp) and zinc-finger protein (792 bp) were generated using the partial sequences obtained from clones in the cDNA library. On the other hand, the partial cDNA sequence (450 bp) of the house keeping gene glyceraldehydes-3-phosphate was obtained using designed gene-specific primers and polymerase chain reaction. The complete cDNAs of two isoforms of the pili oleisin were elucidated. RAPD-PCR [randomized amplified polymorphic DNA-polymerase chain reaction] using 37 random primers has been conducted to screen for sex-specific and sex-neutral DNA fragments for the development of SCAR [sequence characterized amplified region] markers for sex determination of pili. Three candidate sex neutral DNA fragments have been obtained from the study." @default.
• W2804229132 created "2018-06-01" @default.
• W2804229132 creator A5035876687 @default.
• W2804229132 creator A5037198637 @default.
• W2804229132 creator A5063925106 @default.
• W2804229132 date "2013-01-01" @default.
• W2804229132 modified "2023-09-23" @default.
• W2804229132 title "Development of DNA markers for genetic diversity analysis, fingerprinting and sex determination of pili (Canarium ovatum)" @default.
• W2804229132 hasPublicationYear "2013" @default.
• W2804229132 type Work @default.
• W2804229132 sameAs 2804229132 @default.
• W2804229132 citedByCount "0" @default.
• W2804229132 crossrefType "journal-article" @default.
• W2804229132 hasAuthorship W2804229132A5035876687 @default.
• W2804229132 hasAuthorship W2804229132A5037198637 @default.
• W2804229132 hasAuthorship W2804229132A5063925106 @default.
• W2804229132 hasConcept C104317684 @default.
• W2804229132 hasConcept C144024400 @default.
• W2804229132 hasConcept C149923435 @default.
• W2804229132 hasConcept C190789776 @default.
• W2804229132 hasConcept C2777461220 @default.
• W2804229132 hasConcept C2908647359 @default.
• W2804229132 hasConcept C54355233 @default.
• W2804229132 hasConcept C552990157 @default.
• W2804229132 hasConcept C59822182 @default.
• W2804229132 hasConcept C79386861 @default.
• W2804229132 hasConcept C81977670 @default.
• W2804229132 hasConcept C84623179 @default.
• W2804229132 hasConcept C86803240 @default.
• W2804229132 hasConceptScore W2804229132C104317684 @default.
• W2804229132 hasConceptScore W2804229132C144024400 @default.
• W2804229132 hasConceptScore W2804229132C149923435 @default.
• W2804229132 hasConceptScore W2804229132C190789776 @default.
• W2804229132 hasConceptScore W2804229132C2777461220 @default.
• W2804229132 hasConceptScore W2804229132C2908647359 @default.
• W2804229132 hasConceptScore W2804229132C54355233 @default.
• W2804229132 hasConceptScore W2804229132C552990157 @default.
• W2804229132 hasConceptScore W2804229132C59822182 @default.
• W2804229132 hasConceptScore W2804229132C79386861 @default.
• W2804229132 hasConceptScore W2804229132C81977670 @default.
• W2804229132 hasConceptScore W2804229132C84623179 @default.
• W2804229132 hasConceptScore W2804229132C86803240 @default.
• W2804229132 hasLocation W28042291321 @default.
• W2804229132 hasOpenAccess W2804229132 @default.
• W2804229132 hasPrimaryLocation W28042291321 @default.
• W2804229132 hasRelatedWork W1495245391 @default.
• W2804229132 hasRelatedWork W1963680145 @default.
• W2804229132 hasRelatedWork W1972005521 @default.
• W2804229132 hasRelatedWork W1980092970 @default.
• W2804229132 hasRelatedWork W1988505755 @default.
• W2804229132 hasRelatedWork W1991191259 @default.
• W2804229132 hasRelatedWork W2012466790 @default.
• W2804229132 hasRelatedWork W2028122287 @default.
• W2804229132 hasRelatedWork W2039377475 @default.
• W2804229132 hasRelatedWork W2062377597 @default.
• W2804229132 hasRelatedWork W2071390073 @default.
• W2804229132 hasRelatedWork W2082760138 @default.
• W2804229132 hasRelatedWork W2089607707 @default.
• W2804229132 hasRelatedWork W2096370583 @default.
• W2804229132 hasRelatedWork W2100900330 @default.
• W2804229132 hasRelatedWork W2125596915 @default.
• W2804229132 hasRelatedWork W2153969861 @default.
• W2804229132 hasRelatedWork W220531203 @default.
• W2804229132 hasRelatedWork W318421641 @default.
• W2804229132 hasRelatedWork W49219026 @default.
• W2804229132 isParatext "false" @default.
• W2804229132 isRetracted "false" @default.
• W2804229132 magId "2804229132" @default.
• W2804229132 workType "article" @default.