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- W2804244604 abstract "Fluoride is a widespread environmental pollutant which can induce low sperm quality and fertilizing ability. However, effect of fluoride on proteomic changes of sperm is unknown. In this study, two-dimensional electrophoresis (2DE) and mass spectrometry (MS) were used to investigate the differently expressed proteins of sperm from mice exposed to fluoride. 180 male mice were randomly divided into three groups, and were administrated with the distilled water containing 0, 25, and 100 mg L−1 NaF, respectively. After 45, 90 and 180 day's exposure, mice were sacrificed and sperm from the cauda epididymis and vas deferens were collected for 2DE. 16 differently expressed spots were picked up to identify using MS, 15 of which were successfully identified. Many of them are associated with the sperm function such as sperm motility, maturation, capacitation and acrosome reaction, lipid peroxidation, detoxification, inflammation, and stability of membrane structure. These results could contribute to the explanation and further research of mechanisms underlying sperm damage induced by fluoride." @default.
- W2804244604 created "2018-06-01" @default.
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- W2804244604 date "2018-09-01" @default.
- W2804244604 modified "2023-10-07" @default.
- W2804244604 title "Proteomic identification of sperm from mice exposed to sodium fluoride" @default.
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- W2804244604 doi "https://doi.org/10.1016/j.chemosphere.2018.05.153" @default.
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