FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804313153> ?p ?o ?g. }

• W2804313153 endingPage "2277" @default.
• W2804313153 startingPage "2265" @default.
• W2804313153 abstract "The α-anomers of 8-aza-2'-deoxyguanosine (αGd*) and 2'-deoxyguanosine (αGd) were site-specifically incorporated in 12-mer duplexes opposite to the four canonical DNA constituents dA, dG, dT, and dC. Oligodeoxyribonucleotides containing αGd* display significant fluorescence at slightly elevated pH (8.0). Oligodeoxyribonucleotides incorporating β-anomeric 8-aza-2'-deoxyguanosine (Gd*) and canonical dG were studied for comparison. For αGd* synthesis, an efficient purification of anomeric 8-azaguanine nucleosides was developed on the basis of protected intermediates, and a new αGd* phosphoramidite was prepared. Differences were observed for sugar conformations ( N vs S) and p Ka values of anomeric nucleosides. Duplex stability and mismatch discrimination were studied employing UV-dependent melting and fluorescence quenching. A gradual fluorescence change takes place in duplex DNA when the α-nucleoside αGd* was positioned opposite to the four canonical β-nucleosides. The strongest fluorescence decrease appeared in duplexes incorporating αGd*-Cd base pair matches. Decreasing fluorescence corresponds to increasing Tm values. For mismatch discrimination, the α-anomers αGd* and αGd are more efficient than the corresponding β-nucleosides. Duplexes with single purine-purine αGd*-αGd* or αGd-αGd base pairs are significantly more stable than those displaying β-d configuration. CD spectra indicate that single mutations by α-anomeric nucleosides do not affect the global structure of B-DNA." @default.
• W2804313153 created "2018-06-01" @default.
• W2804313153 creator A5023319759 @default.
• W2804313153 creator A5060869360 @default.
• W2804313153 creator A5087408245 @default.
• W2804313153 date "2018-05-17" @default.
• W2804313153 modified "2023-09-26" @default.
• W2804313153 title "Guanine and 8-Azaguanine in Anomeric DNA Hybrid Base Pairs: Stability, Fluorescence Sensing, and Efficient Mismatch Discrimination with α-<scp>d</scp>-Nucleosides" @default.
• W2804313153 cites W1151769068 @default.
• W2804313153 cites W1962972136 @default.
• W2804313153 cites W1964603841 @default.
• W2804313153 cites W1965577740 @default.
• W2804313153 cites W1966364579 @default.
• W2804313153 cites W1970274082 @default.
• W2804313153 cites W1971039535 @default.
• W2804313153 cites W1972294447 @default.
• W2804313153 cites W1973167452 @default.
• W2804313153 cites W1975601173 @default.
• W2804313153 cites W1979770681 @default.
• W2804313153 cites W1979965386 @default.
• W2804313153 cites W1981823536 @default.
• W2804313153 cites W1983179063 @default.
• W2804313153 cites W1986307323 @default.
• W2804313153 cites W1989920813 @default.
• W2804313153 cites W2004342444 @default.
• W2804313153 cites W2008462901 @default.
• W2804313153 cites W2015578304 @default.
• W2804313153 cites W2020663141 @default.
• W2804313153 cites W2021861488 @default.
• W2804313153 cites W2023226798 @default.
• W2804313153 cites W2026892887 @default.
• W2804313153 cites W2043330847 @default.
• W2804313153 cites W2046732331 @default.
• W2804313153 cites W2054946597 @default.
• W2804313153 cites W2055520900 @default.
• W2804313153 cites W2057666290 @default.
• W2804313153 cites W2057967464 @default.
• W2804313153 cites W2061120056 @default.
• W2804313153 cites W2062091198 @default.
• W2804313153 cites W2066084515 @default.
• W2804313153 cites W2069798717 @default.
• W2804313153 cites W2075537479 @default.
• W2804313153 cites W2080998689 @default.
• W2804313153 cites W2083436750 @default.
• W2804313153 cites W2085393878 @default.
• W2804313153 cites W2085667135 @default.
• W2804313153 cites W2089965913 @default.
• W2804313153 cites W2091354942 @default.
• W2804313153 cites W2093240598 @default.
• W2804313153 cites W2106426977 @default.
• W2804313153 cites W2128061605 @default.
• W2804313153 cites W2134213403 @default.
• W2804313153 cites W2151432332 @default.
• W2804313153 cites W2165522817 @default.
• W2804313153 cites W2216488207 @default.
• W2804313153 cites W2312886680 @default.
• W2804313153 cites W2323695664 @default.
• W2804313153 cites W2328519748 @default.
• W2804313153 cites W2334290361 @default.
• W2804313153 cites W2342801776 @default.
• W2804313153 cites W2519639544 @default.
• W2804313153 cites W2605311752 @default.
• W2804313153 cites W2615741776 @default.
• W2804313153 cites W2726928925 @default.
• W2804313153 cites W2754743957 @default.
• W2804313153 cites W2766432068 @default.
• W2804313153 cites W2789819129 @default.
• W2804313153 cites W2792263257 @default.
• W2804313153 cites W2951567342 @default.
• W2804313153 cites W4212831456 @default.
• W2804313153 cites W4300144458 @default.
• W2804313153 cites W53086750 @default.
• W2804313153 doi "https://doi.org/10.1021/acs.bioconjchem.8b00261" @default.
• W2804313153 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29771499" @default.
• W2804313153 hasPublicationYear "2018" @default.
• W2804313153 type Work @default.
• W2804313153 sameAs 2804313153 @default.
• W2804313153 citedByCount "6" @default.
• W2804313153 countsByYear W28043131532019 @default.
• W2804313153 countsByYear W28043131532020 @default.
• W2804313153 countsByYear W28043131532022 @default.
• W2804313153 crossrefType "journal-article" @default.
• W2804313153 hasAuthorship W2804313153A5023319759 @default.
• W2804313153 hasAuthorship W2804313153A5060869360 @default.
• W2804313153 hasAuthorship W2804313153A5087408245 @default.
• W2804313153 hasConcept C104317684 @default.
• W2804313153 hasConcept C121332964 @default.
• W2804313153 hasConcept C129312508 @default.
• W2804313153 hasConcept C185581394 @default.
• W2804313153 hasConcept C185592680 @default.
• W2804313153 hasConcept C2776023528 @default.
• W2804313153 hasConcept C2776043002 @default.
• W2804313153 hasConcept C2776543447 @default.
• W2804313153 hasConcept C2778301229 @default.
• W2804313153 hasConcept C2780441211 @default.
• W2804313153 hasConcept C512185932 @default.
• W2804313153 hasConcept C552990157 @default.
• W2804313153 hasConcept C55493867 @default.

• next