Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804433306> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2804433306 endingPage "93" @default.
- W2804433306 startingPage "90" @default.
- W2804433306 abstract "Objective of this work was the cloning of Vibrio cholerae hlyA gene in a plasmid vector providing expression of foreign genes under the control of T5 promoter, and construction of E. coli strain – super-producer of Vibrio cholerae recombinant hemolysin. Materials and methods . V. cholerae о1 strain served as a DNA donor, pQE30 – as a vector plasmid. The gene was PCR-amplified, cloning was carried out by means of conventional methods, productivity of recombinants and localization of the required protein was determined based on the results of electrophoresis of cell lysates. Results and conclusions. A recombinant plasmid pHlyA, expressing the cloned hlyA gene of Vibrio cholerae El Tor under the control of T5 promoter after IPTG induction, has been constructed. Carrying this plasmid strain E. coli M15[pREP4]pHlyA is the super-producer of hemolysin: the content of the product in whole cell lysates is up to 13 %, and in inclusion bodies – up to 17 % of the total cell proteins. The product of the cloned gene, in spite of the absence of proteolytic processing and presence of the hexahistidine block (6His-tag) at its N-terminus, possesses hemolytic activity towards sheep erythrocytes. 6His-tag will provide for obtaining a purified preparation on specific sorbents with a view to create diagnosticums as well as to study the significance of hemolysin as a pathogenicity/persistence factor. The advantages of this producer are the high output of the required protein, inability of synthesis of any accessory biologically active substances, short-term period of biomass growing (4–6 h including induction) and possibility of culturing without sticking to the guidelines for work with the agents of particularly dangerous infections." @default.
- W2804433306 created "2018-06-01" @default.
- W2804433306 creator A5007944474 @default.
- W2804433306 creator A5053646094 @default.
- W2804433306 creator A5063359646 @default.
- W2804433306 creator A5075804557 @default.
- W2804433306 date "2018-04-07" @default.
- W2804433306 modified "2023-09-27" @default.
- W2804433306 title "Escherichia coli Strain – Super-Producer of Vibrio cholerae Hemolysin" @default.
- W2804433306 doi "https://doi.org/10.21055/0370-1069-2018-1-90-93" @default.
- W2804433306 hasPublicationYear "2018" @default.
- W2804433306 type Work @default.
- W2804433306 sameAs 2804433306 @default.
- W2804433306 citedByCount "1" @default.
- W2804433306 countsByYear W28044333062019 @default.
- W2804433306 crossrefType "journal-article" @default.
- W2804433306 hasAuthorship W2804433306A5007944474 @default.
- W2804433306 hasAuthorship W2804433306A5053646094 @default.
- W2804433306 hasAuthorship W2804433306A5063359646 @default.
- W2804433306 hasAuthorship W2804433306A5075804557 @default.
- W2804433306 hasBestOaLocation W28044333061 @default.
- W2804433306 hasConcept C104317684 @default.
- W2804433306 hasConcept C121050878 @default.
- W2804433306 hasConcept C136139683 @default.
- W2804433306 hasConcept C150194340 @default.
- W2804433306 hasConcept C153911025 @default.
- W2804433306 hasConcept C19924922 @default.
- W2804433306 hasConcept C199360897 @default.
- W2804433306 hasConcept C22744801 @default.
- W2804433306 hasConcept C2776154503 @default.
- W2804433306 hasConcept C2778643871 @default.
- W2804433306 hasConcept C40767141 @default.
- W2804433306 hasConcept C41008148 @default.
- W2804433306 hasConcept C523546767 @default.
- W2804433306 hasConcept C54355233 @default.
- W2804433306 hasConcept C547475151 @default.
- W2804433306 hasConcept C60987743 @default.
- W2804433306 hasConcept C86803240 @default.
- W2804433306 hasConcept C89423630 @default.
- W2804433306 hasConceptScore W2804433306C104317684 @default.
- W2804433306 hasConceptScore W2804433306C121050878 @default.
- W2804433306 hasConceptScore W2804433306C136139683 @default.
- W2804433306 hasConceptScore W2804433306C150194340 @default.
- W2804433306 hasConceptScore W2804433306C153911025 @default.
- W2804433306 hasConceptScore W2804433306C19924922 @default.
- W2804433306 hasConceptScore W2804433306C199360897 @default.
- W2804433306 hasConceptScore W2804433306C22744801 @default.
- W2804433306 hasConceptScore W2804433306C2776154503 @default.
- W2804433306 hasConceptScore W2804433306C2778643871 @default.
- W2804433306 hasConceptScore W2804433306C40767141 @default.
- W2804433306 hasConceptScore W2804433306C41008148 @default.
- W2804433306 hasConceptScore W2804433306C523546767 @default.
- W2804433306 hasConceptScore W2804433306C54355233 @default.
- W2804433306 hasConceptScore W2804433306C547475151 @default.
- W2804433306 hasConceptScore W2804433306C60987743 @default.
- W2804433306 hasConceptScore W2804433306C86803240 @default.
- W2804433306 hasConceptScore W2804433306C89423630 @default.
- W2804433306 hasIssue "1" @default.
- W2804433306 hasLocation W28044333061 @default.
- W2804433306 hasLocation W28044333062 @default.
- W2804433306 hasOpenAccess W2804433306 @default.
- W2804433306 hasPrimaryLocation W28044333061 @default.
- W2804433306 hasRelatedWork W1954988256 @default.
- W2804433306 hasRelatedWork W196040692 @default.
- W2804433306 hasRelatedWork W2055925233 @default.
- W2804433306 hasRelatedWork W2075548247 @default.
- W2804433306 hasRelatedWork W2130789203 @default.
- W2804433306 hasRelatedWork W2310099197 @default.
- W2804433306 hasRelatedWork W2364744239 @default.
- W2804433306 hasRelatedWork W2412926707 @default.
- W2804433306 hasRelatedWork W2415315657 @default.
- W2804433306 hasRelatedWork W3094141947 @default.
- W2804433306 isParatext "false" @default.
- W2804433306 isRetracted "false" @default.
- W2804433306 magId "2804433306" @default.
- W2804433306 workType "article" @default.