FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804504328> ?p ?o ?g. }

• W2804504328 endingPage "3986" @default.
• W2804504328 startingPage "3976" @default.
• W2804504328 abstract "The human zinc transporter SLC39A2, also known as ZIP2, was shown to mediate zinc transport that could be inhibited at pH <7.0 and stimulated by HCO3–, suggesting a Zn2+/HCO3– cotransport mechanism [Gaither, L. A., and Eide, D. J. (2000) J. Biol. Chem. 275, 5560–5564]. In contrast, recent experiments in our laboratory indicated that the functional activity of ZIP2 increases at acidic pH [Franz, M. C., et al. (2014) J. Biomol. Screening 19, 909–916]. The study presented here was therefore designed to reexamine the findings about the pH dependence and to extend the functional characterization of ZIP2. Our current results show that ZIP2-mediated transport is modulated by extracellular pH but independent of the H+ driving force. Also, in our experiments, ZIP2-mediated transport is not modulated by extracellular HCO3–. Moreover, a high extracellular [K+], which induces depolarization, inhibited ZIP2-mediated transport, indicating that the transport mechanism is voltage-dependent. We also show that ZIP2 mediates the uptake of Cd2+ (Km ∼ 1.57 μM) in a pH-dependent manner (KH+ ∼ 66 nM). Cd2+ transport is inhibited by extracellular [Zn2+] (IC50 ∼ 0.32 μM), [Cu2+] (IC50 ∼ 1.81 μM), and to a lesser extent [Co2+], but not by [Mn2+] or [Ba2+]. Fe2+ is not transported by ZIP2. Accordingly, the substrate selectivity of ZIP2 decreases in the following order: Zn2+ > Cd2+ ≥ Cu2+ > Co2+. Altogether, we propose that ZIP2 is a facilitated divalent metal ion transporter that can be modulated by extracellular pH and membrane potential. Given that ZIP2 expression has been reported in acidic environments [Desouki, M. M., et al. (2007) Mol. Cancer 6, 37; Inoue, Y., et al. (2014) J. Biol. Chem. 289, 21451–21462; Tao, Y. T., et al. (2013) Mol. Biol. Rep. 40, 4979–4984], we suggest that the herein described H+-mediated regulatory mechanism might be important for determining the velocity and direction of the transport process." @default.
• W2804504328 created "2018-06-01" @default.
• W2804504328 creator A5014624546 @default.
• W2804504328 creator A5028288545 @default.
• W2804504328 creator A5029263388 @default.
• W2804504328 creator A5036970079 @default.
• W2804504328 creator A5040861278 @default.
• W2804504328 creator A5041914018 @default.
• W2804504328 creator A5078842111 @default.
• W2804504328 creator A5087475311 @default.
• W2804504328 date "2018-05-23" @default.
• W2804504328 modified "2023-10-14" @default.
• W2804504328 title "Reassessment of the Transport Mechanism of the Human Zinc Transporter SLC39A2" @default.
• W2804504328 cites W1521516049 @default.
• W2804504328 cites W174095185 @default.
• W2804504328 cites W1973325515 @default.
• W2804504328 cites W1974614788 @default.
• W2804504328 cites W1978298108 @default.
• W2804504328 cites W1979377912 @default.
• W2804504328 cites W1986171863 @default.
• W2804504328 cites W1994270106 @default.
• W2804504328 cites W1995312949 @default.
• W2804504328 cites W1997472806 @default.
• W2804504328 cites W2006849038 @default.
• W2804504328 cites W2009205727 @default.
• W2804504328 cites W2019817177 @default.
• W2804504328 cites W2020318903 @default.
• W2804504328 cites W2025308267 @default.
• W2804504328 cites W2027150047 @default.
• W2804504328 cites W2033461453 @default.
• W2804504328 cites W2037403444 @default.
• W2804504328 cites W2037698809 @default.
• W2804504328 cites W2038179116 @default.
• W2804504328 cites W2068038389 @default.
• W2804504328 cites W2070584671 @default.
• W2804504328 cites W2074631079 @default.
• W2804504328 cites W2080645415 @default.
• W2804504328 cites W2081337005 @default.
• W2804504328 cites W2094020675 @default.
• W2804504328 cites W2108307502 @default.
• W2804504328 cites W2112310296 @default.
• W2804504328 cites W2118170233 @default.
• W2804504328 cites W2120196934 @default.
• W2804504328 cites W2121898464 @default.
• W2804504328 cites W2129559645 @default.
• W2804504328 cites W2131247679 @default.
• W2804504328 cites W2145286433 @default.
• W2804504328 cites W2154084988 @default.
• W2804504328 cites W2155019993 @default.
• W2804504328 cites W2158819025 @default.
• W2804504328 cites W2163220299 @default.
• W2804504328 cites W2168338648 @default.
• W2804504328 cites W2170930251 @default.
• W2804504328 cites W2308711581 @default.
• W2804504328 cites W2531494513 @default.
• W2804504328 cites W2580655100 @default.
• W2804504328 cites W2736179229 @default.
• W2804504328 cites W2777719338 @default.
• W2804504328 cites W3216995939 @default.
• W2804504328 cites W2307169849 @default.
• W2804504328 doi "https://doi.org/10.1021/acs.biochem.8b00511" @default.
• W2804504328 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7218795" @default.
• W2804504328 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29791142" @default.
• W2804504328 hasPublicationYear "2018" @default.
• W2804504328 type Work @default.
• W2804504328 sameAs 2804504328 @default.
• W2804504328 citedByCount "20" @default.
• W2804504328 countsByYear W28045043282018 @default.
• W2804504328 countsByYear W28045043282019 @default.
• W2804504328 countsByYear W28045043282020 @default.
• W2804504328 countsByYear W28045043282021 @default.
• W2804504328 countsByYear W28045043282022 @default.
• W2804504328 countsByYear W28045043282023 @default.
• W2804504328 crossrefType "journal-article" @default.
• W2804504328 hasAuthorship W2804504328A5014624546 @default.
• W2804504328 hasAuthorship W2804504328A5028288545 @default.
• W2804504328 hasAuthorship W2804504328A5029263388 @default.
• W2804504328 hasAuthorship W2804504328A5036970079 @default.
• W2804504328 hasAuthorship W2804504328A5040861278 @default.
• W2804504328 hasAuthorship W2804504328A5041914018 @default.
• W2804504328 hasAuthorship W2804504328A5078842111 @default.
• W2804504328 hasAuthorship W2804504328A5087475311 @default.
• W2804504328 hasBestOaLocation W28045043282 @default.
• W2804504328 hasConcept C104317684 @default.
• W2804504328 hasConcept C12554922 @default.
• W2804504328 hasConcept C129968341 @default.
• W2804504328 hasConcept C149011108 @default.
• W2804504328 hasConcept C178790620 @default.
• W2804504328 hasConcept C185592680 @default.
• W2804504328 hasConcept C188053792 @default.
• W2804504328 hasConcept C28406088 @default.
• W2804504328 hasConcept C523546767 @default.
• W2804504328 hasConcept C535196362 @default.
• W2804504328 hasConcept C537181965 @default.
• W2804504328 hasConcept C54355233 @default.
• W2804504328 hasConcept C55493867 @default.
• W2804504328 hasConcept C58123911 @default.
• W2804504328 hasConcept C74884574 @default.

• next