FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2804546095> ?p ?o ?g. }

• W2804546095 endingPage "751" @default.
• W2804546095 startingPage "751" @default.
• W2804546095 abstract "Arjona and de Baaij (2018) nicely summarize the history of cyclin M (CNNM) research. However, we do not agree with their conclusion that CNNM proteins are not Na+/Mg2+ exchangers. Their conclusion is primarily based on the following observations and reports: (1) CNNM proteins form dimers of three transmembrane domains, which would be too small to function as a channel/transporter, (2) two groups have reported that Mg2+ efflux was not observed in Mg2+-imaging experiments, and (3) Mg2+ influx was observed in the authors’ experiments using 25Mg2+, irrespective of the quinidine treatment or absence of extracellular Na+. We here make our rebuttal to each of these points. We reported that CNNM2 proteins (∼100 kDa) form SDS-resistant oligomers, which migrated far above the 250 kDa marker by SDS-PAGE (Funato et al. 2017). Sponder et al. (2016) showed similar results. Furthermore, very large CNNM2-containing complexes (>500 kDa) were observed by native PAGE. More experiments are necessary to determine the number of CNNM protomers within each complex. Hardy et al. (2015) performed Mg2+-imaging experiments by expressing CNNM3. However, only the effects of mutations that abolished the interaction with PRL were shown. No control experiments were performed (e.g. transfection of empty vector or non-functional mutants), and their experiments do not provide information regarding CNNM3 function itself. Sponder et al. (2016) expressed CNNM2, but immunofluorescence analyses showed the expressed proteins primarily localized at the intracellular membrane, not the plasma membrane. Therefore, effects on Mg2+ efflux, which occurs across the plasma membrane, cannot be evaluated. We propose that the influx of extracellular 25Mg2+ through TRPM7 was stimulated by Mg2+ depletion caused by CNNM2 expression. Quinidine can inhibit many proteins, such as various cation channels, and is not a general inhibitor for Na+/Mg2+ exchangers. Therefore, the inability of quinidine to inhibit 25Mg2+ influx does not indicate that Na+/Mg2+ exchangers are not involved in the process. Also, removal of extracellular Na+ would not significantly impact Mg2+ influx if it was performed simultaneously with 25Mg2+ addition, because intracellular Mg2+ levels would have already been reduced significantly by Mg2+ efflux by CNNM2 before measurements were recorded. Finally, the Kd value of the interaction between CNNM2 cystathionine-β-synthase domains and Mg-ATP is 0.16 mm (Hirata et al. 2014), which is far below the intracellular concentration of Mg-ATP (∼5 mm). Therefore, contrary to the authors’ model, the ability of CNNM proteins to sense intracellular Mg-ATP fluctuations through this interaction is unlikely. Readers are invited to give their views on this and the accompanying CrossTalk articles in this issue by submitting a brief (250 word) comment. Comments may be submitted up to 6 weeks after publication of the article, at which point the discussion will close and the CrossTalk authors will be invited to submit a ‘LastWord’. Please email your comment, including a title and a declaration of interest, to jphysiol@physoc.org. Comments will be moderated and accepted comments will be published online only as ‘supporting information’ to the original debate articles once discussion has closed. Disclaimer: Supporting information has been peer-reviewed but not copyedited. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. None declared. All authors have approved the final version of the manuscript and agree to be accountable for all aspects of the work. All persons designated as authors qualify for authorship, and all those who qualify for authorship are listed. This work was supported by JSPS KAKENHI JP26460364 and JP17K19396 to Y.F., and JP26291042, JP26111007, JP16K14723 and JP17H04041 to H.M." @default.
• W2804546095 created "2018-06-01" @default.
• W2804546095 creator A5003411661 @default.
• W2804546095 creator A5058440057 @default.
• W2804546095 creator A5071657484 @default.
• W2804546095 creator A5084693611 @default.
• W2804546095 date "2018-01-31" @default.
• W2804546095 modified "2023-10-14" @default.
• W2804546095 title "Rebuttal from Yosuke Funato, Kazuharu Furutani, Yoshihisa Kurachi and Hiroaki Miki" @default.
• W2804546095 cites W1966980676 @default.
• W2804546095 cites W2058096544 @default.
• W2804546095 cites W2312344834 @default.
• W2804546095 cites W2562010912 @default.
• W2804546095 cites W2792060787 @default.
• W2804546095 doi "https://doi.org/10.1113/jp275706" @default.
• W2804546095 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5830423" @default.
• W2804546095 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29383723" @default.
• W2804546095 hasPublicationYear "2018" @default.
• W2804546095 type Work @default.
• W2804546095 sameAs 2804546095 @default.
• W2804546095 citedByCount "7" @default.
• W2804546095 countsByYear W28045460952019 @default.
• W2804546095 countsByYear W28045460952020 @default.
• W2804546095 countsByYear W28045460952021 @default.
• W2804546095 countsByYear W28045460952022 @default.
• W2804546095 crossrefType "journal-article" @default.
• W2804546095 hasAuthorship W2804546095A5003411661 @default.
• W2804546095 hasAuthorship W2804546095A5058440057 @default.
• W2804546095 hasAuthorship W2804546095A5071657484 @default.
• W2804546095 hasAuthorship W2804546095A5084693611 @default.
• W2804546095 hasBestOaLocation W28045460951 @default.
• W2804546095 hasConcept C104317684 @default.
• W2804546095 hasConcept C12554922 @default.
• W2804546095 hasConcept C143065580 @default.
• W2804546095 hasConcept C153911025 @default.
• W2804546095 hasConcept C170493617 @default.
• W2804546095 hasConcept C185592680 @default.
• W2804546095 hasConcept C200082930 @default.
• W2804546095 hasConcept C24530287 @default.
• W2804546095 hasConcept C28406088 @default.
• W2804546095 hasConcept C41625074 @default.
• W2804546095 hasConcept C54009773 @default.
• W2804546095 hasConcept C55493867 @default.
• W2804546095 hasConcept C79879829 @default.
• W2804546095 hasConcept C86803240 @default.
• W2804546095 hasConceptScore W2804546095C104317684 @default.
• W2804546095 hasConceptScore W2804546095C12554922 @default.
• W2804546095 hasConceptScore W2804546095C143065580 @default.
• W2804546095 hasConceptScore W2804546095C153911025 @default.
• W2804546095 hasConceptScore W2804546095C170493617 @default.
• W2804546095 hasConceptScore W2804546095C185592680 @default.
• W2804546095 hasConceptScore W2804546095C200082930 @default.
• W2804546095 hasConceptScore W2804546095C24530287 @default.
• W2804546095 hasConceptScore W2804546095C28406088 @default.
• W2804546095 hasConceptScore W2804546095C41625074 @default.
• W2804546095 hasConceptScore W2804546095C54009773 @default.
• W2804546095 hasConceptScore W2804546095C55493867 @default.
• W2804546095 hasConceptScore W2804546095C79879829 @default.
• W2804546095 hasConceptScore W2804546095C86803240 @default.
• W2804546095 hasFunder F4320334764 @default.
• W2804546095 hasIssue "5" @default.
• W2804546095 hasLocation W28045460951 @default.
• W2804546095 hasLocation W28045460952 @default.
• W2804546095 hasLocation W28045460953 @default.
• W2804546095 hasOpenAccess W2804546095 @default.
• W2804546095 hasPrimaryLocation W28045460951 @default.
• W2804546095 hasRelatedWork W101867907 @default.
• W2804546095 hasRelatedWork W1976376051 @default.
• W2804546095 hasRelatedWork W1991349981 @default.
• W2804546095 hasRelatedWork W2014034588 @default.
• W2804546095 hasRelatedWork W2030915532 @default.
• W2804546095 hasRelatedWork W2043251937 @default.
• W2804546095 hasRelatedWork W2085920417 @default.
• W2804546095 hasRelatedWork W2113632086 @default.
• W2804546095 hasRelatedWork W2408647218 @default.
• W2804546095 hasRelatedWork W2410085250 @default.
• W2804546095 hasVolume "596" @default.
• W2804546095 isParatext "false" @default.
• W2804546095 isRetracted "false" @default.
• W2804546095 magId "2804546095" @default.
• W2804546095 workType "article" @default.